Genetyczne podobieństwo opornych na wankomycynę szczepów Enterococcus faecium izolowanych z materiału klinicznego

• Autorzy: Mlynarczyk A. , Grzybowska W. , Mrowka A. , Tyski S. , Mlynarczyk G.

Warianty tytułu

EN

Genetic similarity of vancomycin resistant strains of Enterococcus faecium isolated from clinical specimens

• Języki publikacji: PL

Abstrakty

PL

Badano występowanie genów vanA, vanB, vanD, vanE i vanG metodą PCR u wankomycyno-opornych szczepów Enterococcus faecium izolowanych z materiału klinicznego pochodzącego z wybranych oddziałów Szpitala Dzieciątka Jezus w Warszawie oraz ustalono podobieństwo szczepów metodą analizy polimorfizmu restrykcyjnych fragmentów genomowego DNA za pomocą (RFLP) - PFGE

EN

Twenty vancomycin resistant E.faecium strains (VRE) isolated from patients of three different hospital wards in 2005 - 2008 were examined. The strains originated from patients of intensive therapy, urological and internistic wards. The chosen wards differ significantly in their specificity. In all cases the presence of o vanA and lack of vanB, vanD, vanE and vanG genes and were found. Strains were compared by using RFLP-PFGE, the reference method for molecular typing of VRE. One group including fourteen strains showing similarity higher than 79.5% was distinguished. This group was divided into subgroups. The greatest similarity was found among strains from patients of intensive therapy ward. Two subgroups of strains showing similarity more than 93.3%, of four strains each were identified. The similarity between these two subgroups was 79.5%. Most strains from other two wards showed less than 79.5% similarity and they could be recognised as not related. Only one strain from internal ward and two strains from urologie ward were similar in 82.1 - 86.4% to one of subgroups of strains originated from intensive therapy.

Słowa kluczowe

PL

material kliniczny; izolaty bakteryjne; Enterococcus faecium; enterokoki; lancuchowa reakcja polimerazy; polimorfizm DNA; podobienstwo genetyczne; opornosc na wankomycyne

• Wydawca: -
• Czasopismo: Medycyna Doświadczalna i Mikrobiologia
• Rocznik: 2010
• Tom: 62
• Numer: 4
• Opis fizyczny: s.297-302,rys.,tab.,bibliogr.

Twórcy

autor

Mlynarczyk A.

• Katedra i Zakład Mikrobiologii Lekarskiej, Warszawski Uniwersytet Medyczny, Warszawa

autor

Grzybowska W.

autor

Mrowka A.

autor

Tyski S.

autor

Mlynarczyk G.

Bibliografia

• 1. Boyd DA, Willey BM, Fawcett D i inni. Molecular characterization of Enterococcus faecal is N06- 0364 with low-level vancomycin resistance harboring a novel D-Ala-D-Ser gene cluster, vanL. Antimicrob Agents Chemother 2008; 2: 2667-72.
• 2. Boyd DA, Du T, Hizon R i inni. VanG-type vancomycin-resistant Enterococcus faecalis strains isolated in Canada. Antimicrob. Agents Chemother 2006; 50: 2217-21.
• 3. Clark NC, Teixeira LM, Facklam RR, Tenover F. Detection and differentiation of vanC-1,vanC-2, and vanC-3 glycopeptide resistance genes in enterococci. J Clin Microbiol 1998; 36: 2294-7.
• 4. Dahl KH, Simonsen GS, Olsvik O, Sundsfjord A. Heterogeneity in the vanB gene cluster of genomically diverse clinical strains of vancomycin-resistant Enterococci. Antimicrob. Agents Chemother 1999,43: 1105-10.
• 5. Depardieu F, Perichon В, Courvalin P. Detection of the van alphabet and identification of enterococci and staphylococci at the species level by multiplex PCR. J Clin Microbiol 2004; 42:5857-60.
• 6. Depardieu, F, Bonora MG, Reynolds PE, Courvalin P. The vanG glycopeptide resistance operon from Enterococcus faecalis revisited. Mol Microbiol 2003; 50: 931-48.
• 7. Depardieu F, Reynolds PE, Courvalin P. VanD-type vancomycin-resistant Enterococcus faecium 10/96A. Antimicrob. Agents Chemother 2003,47, 7-18.
• 8. Dutka-Malen S, Evers S, Courvalin P. Detection of glycopeptide resistance genotypes and identification to the species level of clinically relevant enterococci by PCR. J Clin Microbiol 1995; 33: 24-7.
• 9. Fines M, Perichon B, Reynolds P i inni. VanE a new type of acquired glycopeptide resistance in Enterococcus faecalis BM4405. Antimicrob Agents Chemother 1999; 43: 2161-64
• 10. Grzybowska W, Młynarczyk A, Mrówka A i inni. Molecular epidemiology of vancomycin-resistant Enterococcus faecalis among patients of transplantology wards. Transpl Proc. 2009; 41(8): 3256-7.
• 11. Młynarczyk A, Grzybowska W, Mrówka A i inni. Molecular epidemiology of vancomycin-resistant Enterococcus faecium infecting recipients of solid organs in the transplant surgery ward in 2005 and 2006. Transpl Proc 2009; 41: 3261-3.
• 12. Młynarczyk A, Młynarczyk B, Kmera-Muszyńska Mi inni. Mechanisms of the resistance and tolerance to beta-lactam and glycopeptide antibiotics in pathogenic gram-positive cocci Mini-Rev Med Chem 2009; 9: 1527-37.
• 13. Młynarczyk G, Grzybowska W, Młynarczyk A i inni. Significant increase in the isolation of glycopeptide-resistant Enterococci from patients hospitalized in the transplant surgery ward in 2004-2005. Transpl Proc 2007; 39:2883-5.
• 14. Młynarczyk G, Grzybowska W, Młynarczyk A i inni. Occurrence of glycopeptide-resistant Enterococci in transplant medicine wards in 2001-2005. Transpl Proc 2007; 39: 2886-9.
• 15. Młynarczyk G, Grzybowska W, Młynarczyk A i inni. Oporność na glikopeptydy szczepów Enterococcus spp. izolowanych w latach 1998-2005 od chorych hospitalizowanych. Med Dośw Mikrobiol 2006; 58: 19-25.
• 16. Shirano M, Takakura S, Yamamoto Mi inni. Regional spread of v an A- or vanB-positive Enterococcus gallinarum in hospitals and long-term care facilities in Kyoto prefecture. Japan Epidemiol Infect 2010; 1: 1-7.
• 17. Watanabe S, Kobayashi, N, Quiñones D i inni. Genetic diversity of the low-level vancomycin resistance gene vanC-2/vanC-3 and identification of a novel vanC subtype (vanC-4) in Enterococcus casseliflavus. Microb Drug Resist 2009; 15: 1-9.
• 18. Xu X, Lin D, Yan G i inni. van M, a new glycopeptide resistance gene cluster found in Enterococcus faecium. Antimicrob Agents Chemother 2010; 54: 4643-7.