Influence of isoflavones on secretion of oestradiol-17beta by bovine granulosa cells in vitro

• Autorzy: Ras M. , Kaminski T. , Janowski T. , Zdunczyk S.
• Języki publikacji: EN

Abstrakty

EN

Granulosa cells were isolated from large (≥8 mm) follicles and incubated for 24 h without or with LH (100 ng/mL), daidzein, genistein or equol in doses of 0.5, 5, 10, and 50 µg/mL each or combination (3:13:39) of these isoflavones at a dose 50 µg/ml. The concentration of oestradiol-17ß in incubation media was measured by RIA. All the isoflavones at used doses decreased significantly basal and LH-stimulated secretion of oestradiol-17ß by granulosa cells in a dose dependent manner. The combination of these isoflavones also inhibited significantly oestradiol-17ß secretion by the cells. These data suggested that decreased secretion of oestradiol-17ß caused by dietary isoflavones could be a reason of silent heat in cows.

Słowa kluczowe

EN

bovine granulosa cell; 17-beta estradiol; granulosa cell; in vitro; isoflavone; secretion; cow; oestradiol zob.estradiol; phytoestrogen

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2010
• Tom: 54
• Numer: 3
• Opis fizyczny: p.339-343,fig.,ref.

Twórcy

autor

Ras M.

• Department of Obstetrics and Pathology of Reproduction, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, 10-719 Olsztyn, Poland

autor

Kaminski T.

autor

Janowski T.

autor

Zdunczyk S.

Bibliografia

• 1. Adams N.R.: Detection of the effects of phytoestrogens on sheep and cattle. J Anim Sci 1995, 73, 1509-1515.
• 2. Barkhem T., Carlsson B., Nilsson Y., Enmark E., Gustafsson J.A., Nilsson S.: Differential response of estrogen receptor α and estrogen receptor ß to partial estrogen agonists/antagonists. Molec Pharmacol 1998, 54, 105-112.
• 3. Dusza L., Ciereszko R., Skarżyński D.J., Nogowski L., Opałka M., Kamińska B., Nynca A., Kraszewski O., Słomczyńska M., Włocławek-Potocka I., Korzekwa A., Pruszyńska-Oszmałek E., Szkudelska K.: Mechanism of phytoestrogen action in reproductive processes of mammals and birds. Reprod Biol 2006, 6 (Suppl. 1), 151- 174.
• 4. Haynes-Johanson D., Lai M., Campen C., Palmer S.: Diverse effects of tyrosine kinase inhibitors on follicle- stimulating hormone-stimulated estradiol and progesterone production from rat granulosa cells in serum-containing medium and serum-free medium containing epidermal growth factor. Biol Reprod 1999, 61, 147-153.
• 5. Hoffmann B., Goes de Pinho T., Schuler G.: Determination of free and conjugated oestrogens in peripheral blood plasma, faeces and urine of cattle throughout pregnancy. Exp Endocrinol Diabetes 1997, 105, 296-303.
• 6. Kamiński T., Siawiys G., Bogacka I., Przała J.: The physiological role of ß-endorphin in porcine ovarian follicles. Reprod Nutr Dev 2000, 40, 63-75.
• 7. Khobandehlou H., Hoffmann B., Pallauf J.: Untersuchungen in Mittelhessen zum Vorkommen von östrogen-wirksamen Inhaltsstoffen im Rinderfütter. Dtsch Tierärztl Wschr 1997, 104, 291-294.
• 8. Liggins J., Bluck L.J.C., Runswick S., Atkinson C., Coward W.A., Bingham S.A.: Daidzein and genistein contents of vegetables. Br J Nutr 2000, 84, 717-725.
• 9. Lundh, T.J.-O.: Metabolism of estrogenic isoflavones in domestic animals. Proc Soc Exp Biol Med 1995, 208, 33- 39.
• 10. McDonald M.F.: Effects of plant oestrogens in ruminants. Proc Nutr Soc NZ 1995, 20, 43-51.
• 11. Miksicek R.J.: Interaction of naturally occurring nonsteroidal estrogens with expressed recombinant human estrogen receptor. J Steroid Biochem Mol Biol 1994, 49, 153-160.
• 12. Murkies A.L., Wilcox G., Davis S.R.: Phytoestrogens. J Clinic Endocrinol 1998, 83, 297-303.
• 13. Nwannenna I.A., Madej A., Lundh T.J.-O., Fredriksson G.: Some effects of red clover silage on ovariectomized heifers. Acta Vet Scand 1994, 35, 173-183.
• 14. Nynca A., Ciereszko R.: Effect of genistein on steroidogenic response of granulosa cell populations from porcine preovulatory follicles. Reprod Biol 2006, 6, 31- 50.
• 15. Nynca A., Kraszewska O., Słomczyńska M., Ciereszko R.: Phytoestrogen action in granulosa cells derived from small ovarian follicles in pigs: steroidogenesis, proliferation and expression of estrogen receptors. The 4th Symposium of the Society for Biology of Reproduction and Joint Polish-Japanese Seminar, 22-24 September, Cracow, Poland, 2005, p. 129.
• 16. Piotrowska K., Wocławek-Potocka I., Bah Mammadu M., Piskuła M., Bober A., Skarżyński D.J.: Phytoestrogens and their metabolites inhibit the sensitivity of the bovine corpus luteum on luteotropic factors. J Reprod Dev 2006, 52, 33-41.
• 17. Rice S., Mason H.D., Whitehead S.A.: Phytoestrogens and their low dose combinations inhibit mRNA expression and activity of aromatase in human granulosa- luteal cells. J Steroid Biochem Mol Biol 2006, 101, 216- 225.
• 18. Schopper D.: Negative Energiebilanz während der Laktation: Konsequenzen für die hormonelle Regulation und Ursache von Dysfunktionen von Reproduktionsphenomen am Beispiel der stille Brunst bei Milchkühen post partum. Habilitationsschrift, Universität Hohenheim, 1990.
• 19. Szafrańska B., Zięcik A., Okrasa S.: Primary antisera against selected steroids or proteins and secondary antisera against γ-globulins - an available tool for studies of reproductive processes. Reprod Biol 1002, 2, 187-203.
• 20. Wocławek-Potocka I., Bah Mamadou M., Korzekwa A., Piskuła M., Wiczkowski W., Depta A., Skarżynski D.J.: Soybean-derived phytoestrogens regulate prostaglandin secretion in endometrium during cattle estrous cycle and early pregnancy. Exp Biol Med 2005, 230, 189-199.
• 21. Zduńczyk S., Piskuła M., Janowski T., Barański W., Raś M.: Concentrations of isoflavones in blood plasma of dairy cows with different incidence of silent heat. Bull Vet Inst Pulawy 2005, 49, 189-191.
• 22. Zduńczyk S., Janowski T., Barański W., Raś M.: Untersuchungen zum Einfluss von Isoflavonen auf die postpartale Ovaraktivität bei Kühen. Tierärztl Umschau 2006, 61, 127-132.
• 23. Zhang J.N.E., Kamping N., Luo Q.K.: Extraction and purification of isoflavones from soybeans and characterization of their estrogenic activities. J Agric Food Chem 2007, 55, 6940-6950.