In vitro development of cat interspecies nuclear transfer using pig`s and cow`s cytoplasm

• Autorzy: Kaedei Y. , Fujiwara A. , Tanihara F. , Namula Z. , Viet V.L. , Otoi T.
• Języki publikacji: EN

Abstrakty

EN

This study was conducted to investigate the influence of recipient cytoplasm on the development of somatic cell nuclear transfer (SCNT) embryos, using recipient oocytes and donor cells that were obtained from cats, cows, and pigs. Bovine and porcine oocytes were collected from ovaries obtained at a slaughterhouse, and cat oocytes were collected from ovaries obtained at local veterinary clinics following ovariohysterectomy. Cumulus cells from oocytes of each species were used as donors. When cat cumulus cells were transferred into cow, pig, and cat oocytes, the percentages of fusion and cleavage in the cow-cat and pig-cat interspecies groups were similar to those in the cat-cat intraspecies group. There were no significant differences in the percentages of fusion and cleavage between the interspecies (cow-cat and pig-cat groups) and intraspecies SCNT embryos (cow-cow and pig-pig groups) in each recipient oocyte species. However, none of the interspecies SCNT embryos developed to the morula and blastocyst stage. The percentages of fusion and cleavage were significantly higher (P<0.05) in cow-cat SCNT embryos than in pig-cat SCNT embryos. In conclusion, bovine and porcine cytoplasm can be used to support the early embryonic development of interspecies SCNT with cat donor nucleus. However, the interspecies SCNT embryos could not develop to the late embryonic stage.

Słowa kluczowe

EN

cat; cloned embryo; cow; cytoplasm; development; embryo; in vitro; interspecies somatic cell nuclear transfer; nuclear transfer; pig; nucleolus-cytoplasm interaction

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2010
• Tom: 54
• Numer: 3
• Opis fizyczny: p.405-408,ref.

Twórcy

autor

Kaedei Y.

• Laboratory of Animal Reproduction, United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi 753-8515, Japan

autor

Fujiwara A.

autor

Tanihara F.

autor

Namula Z.

autor

Viet V.L.

autor

Otoi T.

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