Human plasma and cerebrospinal fibronectins differ in the accessibility of the epitopes on the N-terminal domains

• Autorzy: Pupek M. , Lemanska-Perek A. , Jasonek J. , Katnik-Prastowska I.
• Języki publikacji: EN

Abstrakty

EN

 Three monoclonal antibodies specific to the central cell-binding and the C- and N-terminal domains of fibronectin (FN) were used to test antigenic epitope accessibility on human plasma and cerebrospinal fibronectins. In the plasma group, the mean N-terminal FN domain immunoreactivity was about one fourth that of the cell-binding and C-terminal domains, whereas in cerebrospinal fluid they were nearly equal. In the presence of 0.5-6 M urea N-terminal domain immunoreactivity in the plasma increased 3-6-fold, but it decreased 0.7-3-fold in the cerebrospinal fluid. Analysis of fibronectin domain immunoreactivities of the cell-binding and N-terminal domains by a panel of specific monoclonal antibodies may reveal N-terminal fibronectin domain accessibility for reaction with biological partner ligand(s) and/or processes in which FN could be implicated. Such determinations may have important clinical implications.

Słowa kluczowe

EN

human plasma; cerebrospinal fluid; accessibility; epitope; N-terminal fibronectin domain; fibronectin; C-terminal fibronectin domain; monoclonal antibody

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2010
• Tom: 57
• Numer: 3
• Opis fizyczny: p.333-337,fig.,ref.

Twórcy

autor

Pupek M.

• Department of Chemistry and Immunochemistry, Medical University of Wroclaw, Wroclaw, Poland

autor

Lemanska-Perek A.

autor

Jasonek J.

autor

Katnik-Prastowska I.

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