EN
The aim of the study was to evaluate the antioxidative potential of blood during standard physical exercise of horses. The study included 114 clinically healthy horses representing different groups: breeding horses (27), recreation horses (22), and sport horses (65). The group of sport horses consisted of race horses (11), trotters (15), and jumping (25) and driving horses (14). The blood was collected from external jugular vein three times: before exercise, immediately after exercise, and after 30-min rest. The following enzymatic and nonenzymatic antioxidative indices were determined: superoxide dismutase, glutathione peroxidase (GPx), catalase, albumin, total bilirubin, uric acid, and total antioxidant status (TAS). The study demonstrated a temporary post exercise mobilisation of antioxidative mechanisms, especially in cases of intensively trained competitive horses. It was demonstrated that among the antioxidants, the activity of GPx showed the high post-exercise changeability, which suggests a great importance of this enzyme in the protection of the organism from the increased generation of reactive oxygen species. The analysis of results of pre- exercise examination indicated higher rest values of main antioxidative enzymes and TAS in horses trained regularly and intensively than those in animals of a small physical activity. These results prove the positive influence of training on antioxidative potential of blood in horses.