Trans fatty acids induce a proinflammatory response in endothelial cells through ROS-dependent nuclear factor-kappaB activation

• Autorzy: Bryk D. , Zapolska-Downar D. , Malecki M. , Hajdukiewicz K. , Sitkiewicz D.
• Języki publikacji: EN

Abstrakty

EN

It has been shown that increased intake of trans fatty acids (TFAs) is associated with a higher risk of cardiovascular disease. In this study, we have investigated the effects of linoelaidic (LA) and elaidic (EA) acids on the proinflammatory response in endothelial cells, a key step in vascular disease. Human aortic endothelial cells (HAECs) were treated with different concentrations (100 µmol/l in most experiments) of LA or EA for different periods of time. The surface protein and mRNA expression of ICAM-1 and VCAM-1 were determined by flow cytometry and real time RT-PCR, respectively. Adhesion of leukocytes to TFA-treated HAECs was evaluated by an adhesion assay. Activation of nuclear factor-B (NF-B) was evaluated by measuring NF-B p65 phosphorylation using flow cytometry. ROS production was determined by the reduction of fluorescent 2',7'-dichlorofluorescein diacetate (DCFH-DA). LA treatment significantly increased protein and mRNA levels of ICAM-1 and VCAM-1, leukocyte adhesion to HAECs, phosphorylation of NF-B and ROS generation. Similar effects were achieved for cells incubated with EA. Experiments with HAECs pretreated with pyrrolidine dithiocarbamate, an inhibitor of NF-B, revealed that both LA and EA-mediated induction of ICAM-1 and VCAM-1 is mainly regulated by NF-B. The ROS production induced by both of the studied acids was inhibited in the presence of diphenyleneiodonium (DPI), a NADPH oxidase inhibitor, suggesting ROS production through the activation of NADPH oxidase. Furthermore, LA or EA-induced ICAM-1 and VCAM-1 expression, activation of NF-B and adhesion of leukocytes to HAECs were abolished in the presence of DPI. Conclusion: TFAs present in our diet have a direct proinflammatory effect, which promotes leukocyte adhesion to the endothelium through ROS-dependent NF-B activation.

Słowa kluczowe

EN

trans fatty acid; endothelial cell; nuclear factor-kappaB; cardiovascular disease; vascular disease; aortic endothelial cell; atherosclerosis; pathogenesis; cell adhesion molecule; NADPH oxidase; reactive oxygen species; adhesion; leucocyte; phosphorylation; flow cytometry; real-time polymerase chain reaction

• Wydawca: -
• Czasopismo: Journal of Physiology and Pharmacology
• Rocznik: 2011
• Tom: 62
• Numer: 2
• Opis fizyczny: p.229-238,fig.,ref.

Twórcy

autor

Bryk D.

• Department of Biochemistry and Clinical Chemistry, The Warsaw Medical University, 1 Banacha Street, 02-097 Warsaw, Poland

autor

Zapolska-Downar D.

autor

Malecki M.

autor

Hajdukiewicz K.

autor

Sitkiewicz D.