Molecular typing of Staphylococcus aureus based on PCR-RFLP of coa gene and RAPD analysis

• Autorzy: Karakulska J. , Pobucewicz A. , Nawrotek P. , Muszynska M. , Furowicz A. J. , Czernomysy-Furowicz D.
• Języki publikacji: EN

Abstrakty

EN

The aim of this study was molecular identification of S. aureus strains isolated from mastitic milk samples and establishing the genetic relationship between strains isolated from cows belonging to the same herd. In all 43 isolated strains the gap gene (930 bp) was amplified, which enabled their affiliation to the Staphylococcus genus to be established. PCR-RFLP with AluI endonuclease of the gap gene as well as nuc (450 bp) and coa (1130 bp) gene amplification allowed precise S. aureus species identification. One hundred percent of the genetic relationship between strains was established via RAPD-PCR and coa-typing.

Słowa kluczowe

EN

molecular typing; Staphylococcus aureus; PCR-RFLP method; coa gene; random amplified polymorphic DNA analysis; mastitis; nuc gene; gap gene; milk sample; species identification

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2011
• Tom: 14
• Numer: 2
• Opis fizyczny: p.285-286,ref.

Twórcy

autor

Karakulska J.

• Department of Immunology, Microbiology and Physiological Chemistry, Faculty of Biotechnologyand Animal Sciences, West Pomeranian University of Technology in Szczecin, Doktora Judyma 24, 71-466 Szczecin, Poland

autor

Pobucewicz A.

autor

Nawrotek P.

autor

Muszynska M.

autor

Furowicz A. J.

autor

Czernomysy-Furowicz D.

Bibliografia

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