Immunohisto- and cytochemical localization of PR-10 proteins induced by heavy metals in lupine roots

• Autorzy: Przymusinski R. , Gzyl J.
• Języki publikacji: EN

Abstrakty

EN

Most of the data concerning PR-10 proteins accumulation in plants under various environmental stress conditions are derived from biochemical analysis of transcript expression or polypeptide accumulation. In the current study, we examined the localization of PR (L1–L6) proteins, belonging to the PR-10 class family, in the root tips of yellow lupine seedlings using histo- and cytochemical methods. Immunohistochemical localization revealed the presence of PR-proteins in all examined yellow lupine root tip tissue, and lead treatment did not change the distribution of proteins in the roots. However, the metal induced a significant increase in the accumulation of PR-proteins in the meristematic cells and procambium tissue. An immunocytochemical approach with immunogold method demonstrated the cytoplasmic nature of the PR-proteins and their increased accumulation after copper treatment. The results confirmed the affiliation of PR (L1– L6) to PR-10 class family proteins, as well as their intracellular localization.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2013
• Tom: 35
• Numer: 05
• Opis fizyczny: p.1707-1711,fig.,ref.

Twórcy

autor

Przymusinski R.

• Laboratory of Plant Ecophysiology, Institute of Experimental Biology, Faculty of Biology, Adam Mickiewicz University in Poznan´, Umultowska 89, 61-614 Poznan´, Poland

autor

Gzyl J.

• Laboratory of Plant Ecophysiology, Institute of Experimental Biology, Faculty of Biology, Adam Mickiewicz University in Poznan´, Umultowska 89, 61-614 Poznan´, Poland

Bibliografia

• Andersen MBS, Hall S, Dragsted LO (2011) Identification of European allergy patterns to the allergen families PR-10, LTP, and profilin from rosaceae fruits. Clin Rev Allergy Immunol 41:1–16
• Bantignies B, Séguin J, Muzac I, Dédaldéchamp F, Gulick P, Ibrahim R (2000) Direct evidence for ribonucleolytic activity of a PR-10- like protein from white lupin roots. Plant Mol Biol 42:871–881
• Carpin S, Laffer S, Schoentgen F, Valenta R, Chénieux JC, Rideau M, Hamdi S (1998) Molecular characterization of a cytokinininducible periwinkle protein showing sequence homology with pathogenesis-related proteins and the bet v 1 allergen family. Plant Mol Biol 36:791–798
• Clemens S (2006) Toxic metal accumulation, responses to exposure and mechanisms of tolerance in plants. Biochimie 88:1707–1719
• Constabel CP, Brisson N (1995) Stigma- and vascular-specific expression of the PR-10a gene of potato: a novel pattern of expression of a pathogenesis-related gene. Mol Plant-Microbe Interact 8:104–113
• Ekramoddoullah AKM (2004) Physiology and molecular biology of a family of pathogenesis-related PR-10 proteins in conifers. J Crop Improv 10:261–280
• Flores T, Alape-Girón A, Flores-Dıaz M, Flores HE (2002) Ocatin. A novel tuber storage protein from the Andean tuber crop oca with antibacterial and antifungal activities. Plant Physiol 128:1291–1302
• Gzyl J, Przymusiński R, Woźny A (1997) Organospecific reactions of yellow lupin seedlings to lead. Acta Soc Bot Pol 66:61–66
• Iturriaga EA, Leech MJ, Barratt DHP, Wang TL (1994) Two ABAresponsive proteins from pea (Pisum sativum L.) are closely related to intracellular pathogenesis-related proteins. Plant Mol Biol 24:235–240
• Moiseyev GP, Fedoreyeva LI, Zhuravlev YN, Yasnetskaya E, Jekel PA, Beintema JJ (1997) Primary structures of two ribonucleases from ginseng calluses: new members of the PR-10 family of intracellular pathogenesis-related plant proteins. FEBS Lett 407:207–210
• Nagajyoti PC, Lee KD, Sreekanth TVM (2010) Heavy metals, occurrence and toxicity for plants: a review. Environ Chem Lett 8:199–216
• Pinto MP, Ricardo CP (1995) Lupinus albus L. pathogenesis-related proteins that show similarity to PR-10 proteins. Plant Physiol 109:1345–1351
• Przymusiński R, Gwóźdź EA (1994) Increased accumulation of the 16 9103 Mr polypeptide on lupin roots exposed to lead, copper and nitrite ions. Environ Exp Bot 34:63–68
• Przymusiński R, Gwóźdź EA (1999) Heavy metal-induced polypeptides in lupin roots are similar to pathogenesis-related proteins. J Plant Physiol 154:703–708
• Przymusiński R, Rucińska R, Gwóźdź EA (1995) The stressstimulated 16 kDa polypeptide from lupin roots has properties of cytosolic Cu:Zn-superoxide dismutase. Environ Exp Bot 35:485–495
• Przymusiński R, Banaszak A, Gwóźdź EA (2001) Organospecific responses of lupin seedlings to lead I. Localization of lead ions and stress proteins. Acta Physiol Plant 23:109–116
• Przymusiński R, Rucińska R, Gwóźdź EA (2004) Increased accumulation of pathogenesis-related proteins in response of lupine roots to various abiotic stresses. Environ Exp Bot 52:53–61
• Przymusiński R, Rucińska-Sobkowiak R, Ilska B, Gwóźdź EA (2007) Organospecific responses of lupin seedlings to lead. Localization of hydrogen peroxide and peroxidase activity. Acta Physiol Plant 29:411–416
• Qiaojuan Y, Xiaowei Q, Zhengqiang J, Shaoqing Y, Lujia H (2008) Characterization of a pathogenesis-related class 10 protein (PR-10) from Astragalus mongholicus with ribonuclease activity. Plant Physiol Biochem 46:93–99
• Rucińska R, Waplak S, Gwóźdź EA (1999) Free radical formation and activity of antioxidant enzymes in lupin roots exposed to lead. Plant Physiol Biochem 37:187–194
• Saraste M, Sibbald PR, Wittinghofer A (1990) The P-loop–a common motif in ATP- and GTP-binding proteins. Trends Biochem Sci 15:430–434
• Sikorski MM (1997) Expression of Lupinus luteus cDNA coding for PR10 protein in Escherichia coli: purification of the recombinant protein for structural and functional studies. Acta Biochim Pol 44:565–578
• Sikorski MXM, Biesiadka J, Kasperska AE, Kopcińska J, Lotocka B, Golinowski WXX, Legocki AB (1999) Expression of genes encoding PR10 class pathogenesis-related proteins is inhibited in yellow lupine root nodules. Plant Sci 149:125–137
• Sobkowiak R, Deckert J (2006) Proteins induced by cadmium in soybean cells. J Plant Physiol 163:1203–1206
• Swoboda I, Scheiner O, Kraft D, Breitenbach M, Heberle-Bors E, Vicente O (1994) A birch gene family encoding pollen allergens and pathogenesis-related proteins. Biochim Biophys Acta, Gene Struct Expr 1219:457–464
• Tamás L, Huttová J, Žigová Z (1997) Accumulation of stress-proteins in intercellular spaces of barley leaves induced by biotic and abiotic factors. Biol Plant 39:387–394
• Utriainen M, Kokko H, Auriola S, Sarrazin O, Karenlampi S (1998) PR-10 protein is induced by copper stress in roots and leaves of a Cu/Zn tolerant clone of birch, Betula pendula. Plant, Cell Environ 21:821–828
• van Loon LC, Pierpoint WS, Boller T, Conejero V (1994) Recommendations for naming plant pathogenesis-related proteins. Plant Mol Biol Rep 12:245–264
• Walter MH, Liu JW, Grand C, Lamb CJ, Hess D (1990) Bean pathogenesis-related (PR) proteins deduced from elicitorinduced transcripts are members of a ubiquitous new class of conserved PR proteins including pollen allergens. Mol Gen Genet 222:353–360
• Walter MH, Liu JW, Wünn J, Hess D (1996) Bean ribonuclease-like pathogenesis-related protein genes (Ypr10) display complex patterns of developmental, dark-induced and exogenous-stimulus-dependent expression. Eur J Biochem 239:281–293

Uwagi

rekord w opracowaniu