Hormonal priming: A terrific approach to improve salt stress tolerance in wheat

• Autorzy: Afzal I. , Basra S. M. A. , Cheema M. A. , Javed M. T.

Warianty tytułu

PL

Hormonalne kondycjonowanie: wspaniałe podejście do poprawy tolerancji na stres solny u pszenicy

• Języki publikacji: EN

Abstrakty

EN

A pot study was conducted in net house to determine the influence of hormonal priming kinetin (50 mg·dm-3), salicylic acid (50 mg·dm-3), spermine (50 mg·dm-3) and spermidine (50 mg·dm-3) on emergence, yield, antioxidants and ionic content of two wheat (Triticum aestivum L.) cultivars SARC-1 (salt tolerant) and MH-97 (salt sensitive) under normal and saline conditions. The growth of wheat plants was severely affected by salinity stress. But the most of hormonal priming strategies were effective in alleviating the adverse effects of salt stress on wheat plants, however, hormonal priming with salicylic acid (SA) followed by priming with kinetin successfully enhanced seedling vigor by increasing emergence index, decreasing the uptake of Na+ and finally increasing grain yield of both cultivars under normal as well as saline conditions. Activities of SOD and CAT in both cultivars were significantly affected by priming in stressful environment. On the other hand, SARC-1 performed better as compared to MH-97 due to the increased antioxidant activities under salt stress. It can be concluded that priming with 50 mg·dm-3 SA followed by 50 mg·dm-3 kinetin for 12 h maximally improved salt tolerance in both wheat cultivars.

PL

Doświadczenie wazonowe zostało przeprowadzone w tunelu ogrodniczym w celu zbadania wpływu hormonalnego kondycjonowania (50 mg·dm-3 kinetyny, 50 50 mg·dm-3 kwasu salicylowego, 50 mg·dm-3 sperminy i 50 mg·dm-3 spermidyny) na rozwój, plon, zawartość jonów oraz antyoksydantów u dwóch odmian pszenicy (Triticum aestivum L.) - SARC-1 (odporna na zasolenie) i MH-97 (wrażliwa na zasolenie). Wzrost pszenicy został bardzo ograniczony przez stres solny. Jednak większość strategii hormonalnego kondycjonowania była skuteczna w łagodzenia szkodliwego wpływu zasolenia na pszenicę. Kondycjonowanie kwasem salicylowym (SA) a następnie kinetyną zwiększyło żywotność siewek poprzez zwiększenie wskaźnika wschodów, obniżenie pobierania Na+ i ostatecznie zwiększając plon obu odmian, zarówno w warunkach kontrolnych, jak i w warunkach zasolenia. Obserwowano znaczący wpływ kondycjonowania na aktywności SOD i CAT dla obu odmian w warunkach stresu. Z drugiej strony, SARC-1 wykazywał lepsze wyniki w porównaniu do MH-97 z powodu większej aktywności antyoksydantów w warunkach stresu solnego. Można wyciągnąć wniosek, iż kondycjonowanie za pomocą kwasu salicylowego o stężeniu 50 mg·dm-3, a następnie kinetyną o stężeniu 50 mg·dm-3 przez 12 godz. maksymalnie poprawia tolerancję na stres solny w przypadku obu badanych odmian pszenicy.

Słowa kluczowe

EN

hormonal priming; salt stress; stress tolerance; plant tolerance; wheat; wheat production; hormone; antioxidant; priming effect

• Wydawca: -
• Czasopismo: Zeszyty Problemowe Postępów Nauk Rolniczych
• Rocznik: 2010
• Tom: 545
• Opis fizyczny: p.81-92,fig.,ref.

Twórcy

autor

Afzal I.

• Department of Crop Physiology, University of Agriculture, Faisalabad-38040, Pakistan

autor

Basra S. M. A.

autor

Cheema M. A.

autor

Javed M. T.

Bibliografia

• Afzal I., Basra S.M.A., Ashraf M., Hameed A., Farooq M. 2006a. Physiological enhancements for alleviation of salt tolerance in spring wheat. Pak. J. Bot. 38: 1649-1659.
• Afzal I., Basra S.M.A., Farooq M., Nawaz A. 2006b. Alleviation of salinity stress in spring wheat by hormonal priming with ABA, salicylic acid and ascorbic acid. Int. J. Agri. Biol. 8: 23-28.
• Afzal I., Rauf S., Basra S.M.A., Murtaza G. 2008. Halopriming improves vigor, metabolism of reserves and ionic contents in wheat seedlings under salt stress. Plant Soil Environ. 54: 382-388.
• Agarwal S., Sairam R.K.G., Srivastava C., Tyagi A., Meena R.C. 2005. Role of ABA, salicylic acid, calcium and hydrogen peroxide on antioxidant enzymes induction in wheat seedlings. Plant Sci. 169: 559-570.
• Ashraf M., Athar H.R., Harris P.J.C., Kwon T.R. 2008. Some prospective strategies for improving crop salt tolerance. Adv. Agron. 97: 45-110.
• Association of Official Seed Analysts 1983. Seed vigor testing handbook publication No. 32. Association of Official Seed Analysts. Springfield, IL.
• Azevedo Neto A.D., Prisco J.T., Eneas-Filho J., Medeiros J.V.R., Gomes-Filho E. 2005. Hydrogen peroxide pre-treatment induces stress acclimation in maize plants. J. Plant Physiol. 162: 1114-22.
• Bailly C., Benamar A., Corbineau F., Côme D. 1998. Free radical scavenging as affected by accelerated ageing and subsequent priming in sunflower seeds. Physiol. Plant. 104: 646-652.
• Basra S.M.A., Afzal I., Anwar S., Shafique M., Haq A., Majeed K. 2006. Effect of different seed invigoration techniques on wheat (Triticum aestivum L.) seeds sown under saline and non-saline conditions. J. Seed Technol. 28: 36-45.
• Bewley J.D., Black M. 1982. Physiology and biochemistry of seeds in relation to germination. Vol. 2. Viability, dormancy, and environmental control. Springer-Verlag, Berlin.
• Chipa B.R., Lal P. 1993. Ionic ratios as the basis of salt tolerance in wheat. Agrochimica 37: 63-67.
• Dixit V., Pandey V., Shyam R. 2001. Differential antioxidative response to cadmium in roots and leaves of pea. J. Exp. Bot. 52: 1101-109.
• Fujikura Y., Karssen C.M. 1995. Molecular studies on osmoprimed seeds of cauliflower: a partial amino acid sequence of a vigour-related protein and osmopriming-enhanced expression of putative aspartic protease. Seed Sci. Res. 5: 177-181.
• Gadallah M.A.A. 1999. Effects of kinetin on growth, grain yield and some mineral elements in wheat plants growing under excess salinity and oxygen deficiency. Plant Growth Regul. 27: 63-71
• Gorham J., McDonnel E., Wyn Jones R.G. 1984. Salt tolerance in the Triticaceae. I. Leymus sabulosus. J. Exp. Bot. 35: 1200-1209.
• Greenway H., Munns R. 1980. Mechanism of salt tolerance in nonhalophytes. Ann. Rev. Plant Physiol. 31: 149-190.
• Hare P.D., Cress W.A., Staden J.V. 1997. The involvement of cytokinins in plant responses to environmental stress. Plant Growth Regul. 23: 79-103.
• Hocart C.H., Lethem D.S., Parker C.W. 1990. Metabolism and translocation of exogenous zeatin riboside in germinating seeds and seedlings of zea mays. J. Exp. Bot. 41: 1517-1524.
• Iqbal M., Ashraf M. 2006. Wheat seed priming in relation to salt tolerance: growth, yield and levels of free salicylic acid and polyamines. Ann. Bot. Fennici 43: 250-259.
• Kampfenkel K., Montagu M.V., Inze D. 1995. Extraction and determination of ascorbate dehydroascorbate from plant tissue. Anal. Biochem. 225: 165-67.
• Lacan D., Durand M. 1996. Na+-K+ exchange at the xylem/symplast boundry. Its significance in the salt sensitivity of soyabean. Plant Physiol. 110: 705-711.
• Lee D.H., Kim Y.S., Lee C.B. 2001. The inductive responses of the antioxidant enzymes by salt stress in the rice (Oryza sativa L.). J. Plant Physiol. 158: 737-45.
• Menezes-Benavente L., Teixeira F.K., Kamei C.L.A., Margis-Pinheiro M. 2004. Salt stress induces altered expression of gene encoding antioxidant enzymes in seedlings of a Brazilian indica rice (Oryza sativa L.). Plant Sci. 2: 323-31.
• Noctor G., Foyer C. 1998. Ascorbate and glutathione: Keeping active oxygen under control. Annu. Rev. Plant Physiol. Plant Mol. Biol. 49: 249-79.
• Palma F., Lluch C., Iribarne C., Garcia-Garrido J.M., Garcia N.A.T. 2009. Combined effect of salicylic acid and salinity on some antioxidant activities, oxidative stress and metabolite accumulation in Phaseolus vulgaris. Plant Growth Regul. 58: 307-316.
• Pandey D.K. 1989. Priming induced alleviation of the effects of natural ageing derived selective leakage of constituents in French bean seeds. Seed Sci. Technol. 17: 391-397.
• Parida A.K., Das A.B. 2005. Salt tolerance and salinity effects on plants: a review. Ecotoxicology Environmental Safety 60: 324-349.
• Sarin M.N., Narayana A. 1968. Effect of soil salinity and growth regulators on germination and seelding metabolism of wheat. Physiol. Plant. 21: 1201-1209.
• Shannon M.C., Grieve C.M. 1999. Tolerance of vegetable crops to salinity. Sci. Hort. 78: 5-38.
• Shim I.S., Momose Y., Yamamoto A., Kim D.W., Usui K. 2003. Inhibition of catalase activity by oxidative stress and its relationship salicylic acid accumulation in plants. Plant Growth Regul. 39: 285-292.
• Vaidyanathan H., Sivakumar P., Chakarbarti R., Thomas G. 2003. Scavenging of reactive oxygen species in NaCl stressed rice (Oryza sativa L.) differential response in salt tolerant and sensitive varieties. Plant Sci. 165: 1411-1418.
• Wahid A., Perveen M., Gelani S., Basra S.M.A. 2007. Pretreatment of seed with H2O2 improves salt tolerance of wheat seedlings by alleviation of oxidative damage and expression of stress proteins. J. Plant Physiol. 164: 283-294.
• Wyn Jones R.G. 1981. Salt tolerance, in: Physiological processes limiting plant productivity. C.B. Jhonson (Ed.), Butterworths Press Ltd., London: 271-292.