LAMP3 plays an oncogenic role in osteosarcoma cells partially by inhibiting TP53

• Autorzy: Liu S. , Yue J. , Du W. , Han J. , Zhang W.
• Języki publikacji: EN

Abstrakty

EN

Background: Osteosarcoma (OS) is a common malignant tumor that predominantly occurs in adolescents. Its most common metastasis is to the lungs. As shown in our earlier study, lysosome-associated membrane glycoprotein 3 (LAMP3) is highly upregulated in metastatic OS. However, its role in the regulation of OS cell viability and apoptosis remains unknown. Methods: We knocked down and overexpressed LAMP3 in OS cells and assessed the cell viability and apoptosis. Then, we investigated the expression of apoptosisassociated genes to identify the downstream gene(s) of LAMP3. Results: Knockdown of LAMP3 significantly inhibited OS cell viability and promoted apoptosis. TP53, which is involved in the apoptosis pathway, was found to be highly upregulated after knockdown of LAMP3. Overexpression of LAMP3 significantly increased cell viability and abrogated apoptosis. Importantly, subsequent knockdown of TP53 partially suppressed the increased OS cell apoptosis induced by the inhibition of LAMP3, suggesting that TP53 is a key functional downstream gene of LAMP3. Conclusions: Our findings suggest that LAMP3 promotes OS cell viability and survival by regulating TP53 expression.

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2018
• Tom: 23
• Opis fizyczny: p.1-11,fig.,ref.

Twórcy

autor

Liu S.

• Department of Bone Traumatology, Yantaishan Hospital, Yantai 264000, Shandong Province, People’s Republic of China

autor

Yue J.

• Department of Bone Traumatology, Yantaishan Hospital, Yantai 264000, Shandong Province, People’s Republic of China

autor

Du W.

• Department of Bone Traumatology, Yantaishan Hospital, Yantai 264000, Shandong Province, People’s Republic of China

autor

Han J.

• Department of Bone Traumatology, Yantaishan Hospital, Yantai 264000, Shandong Province, People’s Republic of China

autor

Zhang W.

• Department of Bone Traumatology, Yantai Hospital of Traditional Chinese Medicine, Yantai 264000, Shandong Province, People’s Republic of China

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Identyfikatory

DOI

10.1186/s11658-018-0099-8