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2008 | 52 | 4 |

Tytuł artykułu

Presence of virulence markers in Polish Streptococcus suis field isolates

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
The objective of this study was to detect proteins related with the virulence of Streptococcus suis (S. suis): muramidase-released protein (MRP), extracellular factor (EF), and suilysin (SLY), using the Western blot procedure. The strains were isolated in Poland from pigs with clinical symptoms and pathological lesions of streptococcosis. Eleven phenotypes were identified among the tested strains of serotype 2. Half of them (43) produced all three proteins, i.e. 40 strains (46.51%) produced standard molecular mass proteins (MRP+ EF+ SLY+) and three isolates (3.49%) produced MRP, EF*, and SLY. Thirteen strains (15.12%) produced two proteins. Twenty-three (26.75%) strains synthesised only one protein. No proteins were detected in seven (8.13%) strains. S. suis isolates belonging to serotypes other than serotype 2 represented six phenotypes. There was no isolate synthesising all the three potential virulence factors in this group. These results show that MRP, EF, and SLY are produced in 50% of the strains. Because these isolates originate from diseased pigs, also other factors than MRP, EF, and SLY must be involved in S. suis virulence.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

52

Numer

4

Opis fizyczny

p.517-522,fig.,ref.

Twórcy

autor
  • Department of Swine Diseases, National Veterinary Research Institute, 24-100 Pulawy, Poland
  • Department of Swine Diseases, National Veterinary Research Institute, 24-100 Pulawy, Poland

Bibliografia

  • 1. Allen A.G., Bolitho S., Lindsay H., Khan S., Bryant C., Norton P., Ward P., Leigh J., Morgan J., Riches H., Eastty S., Maskeli D.: Generation and characterization of defined mutant of Streptococcus suis lacking suilysin. Infect Immun 2001, 69, 2732-2735.
  • 2. Berthelot-Herault F., Morvan H., Keribin A.M., Gottschalk M., Kobisch M.: Production of muramidase- released protein (MRP), extracellular factor (EF) and suilysin by field isolates of Streptococcus suis capsular types 2, 1/ 2, 9, 7 and 3 isolated from swine in France. Vet Res 2000, 31, 473-479.
  • 3. Chatellier S., Gottschalk M., Higgins R., Brousseau R., Harel J.: Relatedness of Streptococcus suis serotype 2 isolates from different geographic origins as evaluated by molecular fingerprinting and phenotyping. J Clin Microbiol 1999, 37, 362-366.
  • 4. Fabisiak M., Kita J., Jędryczko R., Binek M.: Prevalence of genes coding virulence factors of Streptococcus suis strains isolated from pigs with symptoms of streptococcosis and from asymptomatic carriers. Medycyna Wet 2006, 62, 785-787.
  • 5. Feder I., Chengappa M.M., Fenwick B., Rider M., Staats J.: Partial characterization of Streptococcus suis type 2 hemolysin. J Clin Microbiol 1994, 32, 1256-1260.
  • 6. Galina L., Pijoan C., Sitjar M., Christianson W.T., Rossow K., Collins J.E.: Interactions between Streptococcus suis serotype 2 and porcine reproductive and respiratory syndrome virus in specific pathogen-free piglets. Vet Rec 1994, 134, 60-64.
  • 7. Galina L., Vecht U., Wisselink H.J., Pijoan C.: Prevalence of various phenotypes of Streptococcus suis isolated from swine in the U.S.A. based on the presence of muraminidase-released protein and extracellular factor. Can J Vet Res 1996, 60, 72-74.
  • 8. Gottschalk M., Lebrun A., Wisselink H.J., Dubreuil J.D., Smith H., Vecht U.: Production of virulence-associated proteins by Canadian strains of Streptococcus suis type 2. Can J Vet Res 1998, 62, 75-79.
  • 9. Gottschalk M., Segura M.: The pathogenesis of the meningitis caused by Streptococcus suis: the unresolved questions. Vet Microbiol 2000, 76, 259-272.
  • 10. Gottschalk M., Segura M.: Streptococcus suis infection in humans: the Chinese experience and the situation in North America. Anim Health Res Rev 2007, 8, 29-45.
  • 11. King S.J., Heath P.J., Luque I., Tarradas C., Dowson C., Whatmore A.: Distribution and genetic diversity of suilysin in Streptococcus suis isolated from different disease of pigs and characterization of the genetic basis of suilysin absence. Infect Immun 2001, 69, 7572-7582.
  • 12. Lun S., Perez-Casal J., Connor W., Willson P.J.: Role of suilysin in pathogenesis of Streptococcus suis capsular serotype 2. Microb Pathog 2003, 34, 27-37.
  • 13. Markowska-Daniel I., Kowalczyk A.: Identification of Streptococcus suis by multiplex PCR. Medycyna Wet 2005, 61, 522-525.
  • 14. Martinez G., Pestana de Castro A.F., Jordao Ribeiro Pagnani K., Nakazato G., Dias da Silvera W., Gottschalk M.: Clonal distribution of an atypical MRP⁺, EF* and suilysin⁺ phenotype of virulent Streptococcus suis serotype 2 strains in Brazil. Can J Vet Res 2003, 67, 52- 55.
  • 15. Okwumabua O., Abdelmagid O., Chengappa M.M.: Hybridization analysis of the gene encoding a hemolysin (suilysin) of Streptococcus suis type 2: evidence for the absence of the gene in some isolates. FEMS Microbiol Lett 1999, 181, 113-121.
  • 16. Smith H.E., Vecht U., Wisselink H.J., Stockhofe- Zurwieden N., Biermann Y., Smits M.A.: Mutants of Streptococcus suis types 1 and 2 impaired in expression of muramidase-released protein and extracellular protein induce disease in newborn germfree pigs. Infect Immun 1996, 64, 4409-4412.
  • 17. Smith H.E., Rijnsburger M., Stockhofe-Zurwieden N., Wisselink H.J., Vecht U., Smits M.A.: Virulent strains of Streptococcus suis serotype 2 and highly virulent strains of Streptococcus suis serotype 1 can be recognized by a unique ribotype profile. J Clin Microbiol 1997, 35, 1049- 1053.
  • 18. Szczotka A., Markowska-Daniel I.: Biochemical features of Polish isolates of Streptococcus suis. Medycyna Wet 2006, 62, 566-569.
  • 19. Vecht U., Wisselink H.J., van Dijk J.E., Smith H.E.: Virulence of Streptococcus suis type 2 strains in newborn germfree pigs depends on phenotype. Infect Immun 1992, 60, 550-556.
  • 20. Vecht U., Wisselink H., Jellema M.L., Smith H.E.: Identification of two proteins associated with virulence of Streptococcus suis type 2. Infect Immun 1991, 59, 3156- 3162.
  • 21. Wisselink H.J., Reek F.H., Vecht U., Stockhofe- Zurwieden N., Smits M.A., Smith H.E.: Detection of virulent strains of Streptococcus suis type 2 and highly virulent strains of Streptococcus suis type 1 in tonsillar specimens of pigs by PCR. Vet Microbiol 1999, 67, 143- 157.
  • 22. Wisselink H.J., Smith H., Stockhofe-Zurwieden, Peperkamp K., Vecht U.: Distribution of capsular types and production of muramidase-released protein (MRP) and extracellular factor (EF) of Streptococcus suis strains isolated from diseased pigs in seven European countries. Vet Microbiol 2000, 74, 237-248.

Typ dokumentu

Bibliografia

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Identyfikator YADDA

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