Identification of proteins regulated by ABA in response to combined drought and heat stress in maize roots

• Autorzy: Liu T , Zhang L. , Yuan Z. , Hu X. , Lu M. , Wang W. , Wang Y.
• Języki publikacji: EN

Abstrakty

EN

We adopted a proteomics approach to identify and analyze the differential expression of maize root proteins associated with abscisic acid (ABA) regulation under combined drought and heat stress. Using mass spectrometry, we identified 22 major proteins that were significantly up-regulated under combined drought and heat stress. These 22 proteins were classified into 6 functional categories: disease/defense (8), metabolism (3), cell growth/ division (3), signal transduction (2), transporters (2) and unclassified (4). Our previous reports showed that ABA regulates the expression of several small heat-shock proteins (sHSPs) in maize leaves subjected to the combination of drought and heat stress; however, no sHSPs were identified among the root proteins up-regulated in this study. RT-PCR and western blot analyses were used to identify six known sHSPs. The maize roots were pretreated with 100 lM of ABA, and subsequently, the expression of the 22 up-regulated proteins and 6 sHSPs was examined. 11 proteins were up-regulated in an ABA-dependent manner, 13 proteins were up-regulated in an ABA-independent manner, and 4 proteins were up-regulated but inhibited by ABA. The up-regulated proteins are interesting candidates for further physiological and molecular investigations of combination stress tolerance in maize.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2013
• Tom: 35
• Numer: 02
• Opis fizyczny: p.501-513,fig.,ref.

Twórcy

autor

Liu T

• Key Laboratory of Physiological Ecology and Genetic Improvement of Food Crops in Henan Province, 450002 Zhengzhou, China
• College of Agronomy, Henan Agricultural University, 450002 Zhengzhou, China

autor

Zhang L.

• College of Life Science, Henan Agricultural University, 450002 Zhengzhou, China

autor

Yuan Z.

• College of Life Science, Henan Agricultural University, 450002 Zhengzhou, China

autor

Hu X.

• Key Laboratory of Physiological Ecology and Genetic Improvement of Food Crops in Henan Province, 450002 Zhengzhou, China
• College of Life Science, Henan Agricultural University, 450002 Zhengzhou, China

autor

Lu M.

• College of Life Science, Henan Agricultural University, 450002 Zhengzhou, China

autor

Wang W.

• Key Laboratory of Physiological Ecology and Genetic Improvement of Food Crops in Henan Province, 450002 Zhengzhou, China
• College of Life Science, Henan Agricultural University, 450002 Zhengzhou, China

autor

Wang Y.

• College of Life Science, Henan Agricultural University, 450002 Zhengzhou, China

Bibliografia

• Al-Whaibi MH (2010) Plant heat-shock proteins: a mini review. J King Saud Univ. doi:10.1016/j.jksus.2010.06.022
• Bevan M, Bancroft I, Bent E, Love K, Goodman H, Dean C, Bergkamp R, Dirkse W, Van Staveren M, Stiekema W et al (1998) Analysis of 1.9 Mb of contiguous sequence from chromosome 4 of Arabidopsis thaliana. Nature 391:485–488
• Bianchi MW, Damerval C, Vartanian N (2002) Identification of proteins regulated by cross-talk between drought and hormone pathways in Arabidopsis wild-type and auxin-insensitive mutants, axr1 and axr2. Funct Plant Biol 29:55–61
• Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254
• Cao SQ, Jiang L, Song SY, Jing R, Xu GS (2006) AtGRP7 is involved in the regulation of abscisic acid and stress responses in Arabidopsis. Cell Mol Biol Lett 11:526–535
• Colditz F, Nyamsuren O, Niehaus K, Eubel H, Braun HP, Krajinski F (2004) Proteomic approach: identification of Medicago truncatula proteins induced in roots after infection with the pathogenic oomycete Aphanomyces euteiches. Plant Mol Biol 55:109–120
• Forsthoefel NR, Cushman MAF, Cushman JC (1995) Posttranscriptional and posttranslational control of enolase expression in the facultative grassulacean acid metabolism plant Mesembryanthemum crystallinum L. Plant Physiol 108:1185–1195
• Guillet-Claude C, Birolleau-Touchard C, Manicacci D, Fourmann M, Barraud S, Carret V, Martinant JP, Barrie`re Y (2004) Genetic diversity associated with variation in silage corn digestibility for three O-methyltransferase genes involved in lignin biosynthesis. Theor Appl Genet 110:126–135
• Haluskova´ L, Valentovicova´ K, Huttova´ J, Mistrı´k I, Tama´s L (2009) Effect of abiotic stresses on glutathione peroxidase and glutathione S-transferase activity in barley root tips. Plant Physiol Biochem 47:1069–1074
• Hashiguchi A, Ahsan N, Komatsu S (2010) Proteomics application of crops in the context of climate changes. Food Res Int 43: 1803–1813
• Hu XL, Jiang MY, Zhang JH, Tan MP, Zhang AY (2008) Cross-talk between Ca2?/CaM and H2O2 in abscisic acid-induced antioxidant defense in leaves of maize plants exposed to water stress. Plant Growth Regul 55:183–198
• Hu XL, Li YH, Li CH, Yang HR, Wang W, Lu MH (2010a) Characterization of small heat shock proteins associated with maize tolerance to combined drought and heat stress. J Plant Growth Regul 29:455–464
• Hu XL, Liu RX, Li YHi, Wang W, Tai FJ, Xue RL, Li CH (2010 b) Heat Shock Protein 70 Regulates the Abscisic Acid–Induced Antioxidant Response of Maize to Drought and Heat Stress Combination. Plant Growth Regul 60:225-235
• Huerta-Ocampo JA, Leo´n-Galva´n MF, Ortega-Cruz LB, Barrera-Pacheco A, De Leo´n-Rodrı´guez A, Mendoza-Herna´ndez G, Barba de la Rosa AP (2011) Water stress induces up-regulation of DOF1 and MIF1 transcription factors and down-regulation of proteins involved in secondary metabolism in amaranth roots (Amaranthus hypochondriacus L.). Plant Biol (Stuttg) 13: 472–482
• Hwang SY, Van-toai TT (1991) Abscisic Acid induces anaerobiosis tolerance in corn. Plant Physiol 97:593–597
• Iglesias-Acostaa M, Martı´nez-Ballestaa MC, Teruelb JA, Carvajala M (2010) The response of broccoli plants to high temperature and possible role of root aquaporins. Environ Exp Bot 68:83–90
• Irigoyen JJ, Emerich DW, Sa´nchez-Dı´az M (1992) Phosphoenolpynivate carboxylase, malate and alcohol dehydrogenase activities in alfalfa (Medicago sativa) nodules under water stress. Physiol Plantarum 84:61–66
• Jiang HW, Liu MJ, Chen IC, Huang CH, Chao LY, Hsieh HL (2010) A glutathione S-transferase regulated by light and hormones participates in the modulation of Arabidopsis seedling development. Plant Physiol 154:1646–1658
• Johnson JR, Cobb BG, Drew MC (1994) Hypoxic induction of anoxia tolerance in roots of Adh1 null Zea mays L. Plant Physiol 105: 61–67
• Jorgensen JA, Nguyen HT (2004) Isolation, sequence and expression of a cDNA encoding a Class I heat shock protein (HSP17.2) in maize. Plant Sci 97:169–175
• Kang DH, Song Gho Y, Suh MK, Kang CH (2002) Highly sensitive and fast protein detection with coomassie brilliant blue in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Bull Korean Chem Soc 23:1511–1512
• Kellos T, Tı´ma´r I, Szila´gyi V, Szalai G, Galiba G, Kocsy G (2008) Stress hormones and abiotic stresses have different effects on antioxidants in maize lines with different sensitivity. Plant Biol (Stuttg) 10:563–572
• Koussevitzky S, Suzuki N, Huntington S, Armijo L, Sha W, Cortes D, Shulaev V, Mittler R (2008) Ascorbate peroxidase 1 plays a key role in the response of Arabidopsis thaliana to stress combination. J Biol Chem 283:34197–34203
• Lee DG, Ahsan N, Lee SH1, Kang KY, Bahk JD, Lee IJ and Lee BH (2007) A proteomic approach in analyzing heat-responsive proteins in rice leaves. Proteomics 7: 3369–3383
• Lindberg S, Banas A, Stymne S (2005) Effects of different cultivation temperatures on plasma membrane ATPase activity and lipid composition of sugar beet roots. Plant Physiol Biochem 43: 261–268
• Liu XZ, Huang BZ (2005) Root physiological factors involved in creeping bentgrass response to high soil temperatures. Environ Exp Bot 53:233–245
• Ma QH, Xu Y (2008) Characterization of a caffeic acid 3-O-methyltransferase from wheat and its function in lignin biosynthesis. Biochimie 90:515–524
• Overpeck JT, Cole JE (2006) Abrupt change in earth’s climate system. Annu Rev Environ Res 31:1–31
• Pingali PL (2001) CIMMYT 1999–2000 Facts and Trends. Meeting world maize needs: technological opportunities and priorities for the public sector. CIMMYT, Mexico City, pp 1–60
• Plomion C, Lalanne C, Claverol S, Meddour H, Kohler A, Bogeat-Triboulot M (2006) Mapping the proteome of poplar and application to the discovery of drought-stress responsive proteins. Proteomics 6:6509–6527
• Rizhsky L, Liang H, Shuman J, Shulaev V, Davletova S (2004) When defense pathways collide. The response of Arabidopsis to a combination of drought and heat stress. Plant Physiol 134: 1683–1696
• Schachtman DP, Goodger JQD (2008) Chemical root to shoot signaling under drought. Trends Plant Sci 13:281–287
• Selote DS, Khanna-Chopra R (2010) Antioxidant response of wheat roots to drought acclimation. Protoplasma 245:153–163
• Sengupta D, Kannan M, Reddy AR (2011) A root proteomics-based insight reveals dynamic regulation of root proteins under progressive drought stress and recovery in Vigna radiata (L.) Wilczek. Planta 233:1111–1127
• Sobhanian H, Razavizadeh R, Nanjo Y, Ehsanpour AA, Jazii FR, Motamed N, Komatsu S (2010) Proteome analysis of soybean leaves, hypocotyls and roots under salt stress. Proteome Sci 8:1–19
• Sridhar J, Eiteman MA, Juergen W, Wiegel JW (2000) Elucidation of enzymes in fermentation pathways used by Clostridium thermosuccinogenes growing on inulin. Appl Environ Microb 66: 246–251
• Sun W, Montagu MV, Verbruggen N (2002) Small heat shock proteins and stress tolerance in plants. Biochim Biophys Acta 1577:1–9
• Tang L, Kim MD, Yang K-S, Kwon SY, Kim SH, Kim JS, Yun DJ, Kwak SS, Lee HS (2008) Enhanced tolerance of transgenic potato plants overexpressing nucleoside diphosphate kinase 2 against multiple environmental stresses. Transgenic Res 17: 705–715
• Torres GAM, Pflieger S, Corre-Menguy F, Mazubert C, Hartmann C and Christine Lelandais-Brie`re C (2006) Identification of novel drought-related mRNAs in common bean roots by differential display RT-PCR. Plant Sci 171:300-307
• Van Der Straeten D, Rodrigues-Pousada RA, Goodman HM, Van Montagu M (1991) Plant enolase: gene structure, expression, and evolution. Plant Cell 3:719–735
• Vandeleur RK, Mayo G, Shelden MC, Gilliham M, Kaiser BN, Tyerman SD (2009) The role of plasma membrane intrinsic protein aquaporins in water transport through roots: diurnal and drought stress responses reveal different strategies between isohydric and anisohydric cultivars of grapevine. Plant Physiol 149:445–460
• Wahid A, Gelani S, Ashraf F, Foolad R (2007) Heat tolerance in plants: an overview. Environ Exp Bot 61:199–223
• Wang W, Vignani R, Scali M, Cresti M (2006) A universal and rapid protocol for protein extraction from recalcitrant plant tissues for proteomic analysis. Electrophoresis 27:2782–2786
• Wang W, Bianchi L, Scali M, Liu LW, Bini L, Cresti M (2009) Proteomic analysis of b-1, 3-glucanase in grape berry tissues. Acta Physiol Plant 31:597–604
• Xie YR, Chen ZY, Brown RL, Bhatnagar D (2010) Expression and functional characterization of two pathogenesis-related protein 10 genes from Zea mays. J Plant Physiol 167:121–130
• Xiong L, Ishitani M, Zhu JK (1999) Interaction of osmotic stress, temperature, and abscisic acid in the regulation of gene expression in Arabidopsis. Plant Physiol 119:205–212
• Xiong L, Wang RG, Mao GH, Jessica M, Koczan JM (2006) Identification of drought tolerance determinants by genetic analysis of root response to drought stress and abscisic acid. Plant Physiol 142:1065–1074
• XuQZ,HuangBG(2001)Lowering soil temperatures improves creeping bentgrass growth under heat stress. Crop Sci 41:1878–1883
• Xu CP, Huang BR (2008) Root proteomic responses to heat stress in two Agrostis grass species contrasting in heat tolerance. J Exp Bot 59:4183–4194
• Xu Y, Gianfagna T, Huang BR (2010) Proteomic changes associated with expression of a gene (ipt) controlling cytokinin synthesis for improving heat tolerance in a perennial grass species. J Exp Bot 61:3273–3289
• Yamaguchi M, Valliyodan B, Zhang J, Lenoble ME, Yu O, Rogers EE, Nguyen HT, Sharp RE (2010) Regulation of growth response to water stress in the soybean primary root. I. Proteomic analysis reveals region-specific regulation of phenylpropanoid metabolism and control of free iron in the elongation zone. Plant Cell Environ 33:223–243
• Yang L, Zheng B, Mao C, Qi X, Liu F, Wu P (2004) Analysis of transcripts that are differentially expressed in three sectors of the rice root system under water deficit. Mol Gen Genomics 272: 433–442
• Yang L, Su N, Wu MC, Wang CW, Hu HT (2011) Proteomics to identify pathogenesis-related proteins in rice roots under water deficit. Biologia 66:477–483
• Yildiz M, Terz-Oglu S (2006) Synthesis of soluble heat shock protein in seminal root tissues of some cultivated and wild wheat genotypes. Acta Biol Hung 57:81–95
• Zhang Y, Wang YX, Jiang LD, Xu Y, Wang YC, Lu DH, Cheng F (2007) Aquaporin JcPIP2 is involved in drought responses in Jatropha curcas. Acta Biochima Biophysica Sin 39:787–794

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