Detection of enterohaemorrhagic Escherichia coli by triplex real time PCR method

• Autorzy: Bielawska-Drozd A. , Cieslik P. , Bartoszcze M. , Mirski T. , Osek J. , Gryko R.
• Języki publikacji: EN

Abstrakty

EN

Triplex real time PCR method for detection of enterohaemorrhagic strains of Escherichia coli in meat samples was described. Total of 36 strains belonging to different serogroups were used. All strains were cultured in brain heart infusion broth and sorbitol MacConkey agar plates under aerobic conditions. Sets of primers and TaqMan probes were prepared using GenBank resources. Using the designed method we were able to detect bacteria in meat samples and determine genotypes for stx1, stx2, and eaeA genes in tested strains, simultaneously. The described method provides a rapid detection of single colonies of this pathogen in minced meat samples.

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2011
• Tom: 55
• Numer: 4
• Opis fizyczny: p.635-639,fig.,ref.

Twórcy

autor

Bielawska-Drozd A.

• Biological Threats Identification and Countermeasure Centre Pulawy, Military Institute of Hygiene and Epidemiology, 01-163 Warsaw, Poland

autor

Cieslik P.

autor

Bartoszcze M.

autor

Mirski T.

autor

Osek J.

autor

Gryko R.

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