Salt stress tolerance of methylotrophic bacteria Methylophilus sp. and Methylobacterium sp. isolated from coal mine spoils

• Autorzy: Giri D.D. , Kumar A. , Shukla P. N. , Singh R. , Singh P. K. , Pandey K. D.
• Języki publikacji: EN

Abstrakty

EN

Two methylotrophic strains of Bina coalmine spoil BNV7b and BRV25 were identified based on physiological traits and 16S rDNA sequence as Methylophilus and Methylobacterium species. The strains exhibited similar carbon utilization but differed in N utilization and their response to the metabolic inhibitors. Methylophilus sp. was less tolerant to salt stress and it viability declined to one tenth within 4 h of incubation in 2M NaCl due to membrane damage and leakage of the intracellular electrolytes as evident from malondiaaldehyde (MDA) assay. In 200 mM NaCl, they exhibited increased superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX) activity while in 500 mM NaCl, enzyme activities declined in Methylophilus sp. and increased in Methylobacterium sp. Among exogenously applied osmoprotectants proline was most efficient; however, polyols (mannitol, sorbitol and glycerol) also supported growth under lethal NaCl concentration.

Słowa kluczowe

EN

salt stress; stress tolerance; methylotrophic bacteria; Methylophilus; Methylobacterium; isolation; coal mine spoil

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2013
• Tom: 62
• Numer: 3
• Opis fizyczny: p.273-280,fig.,ref.

Twórcy

autor

Giri D.D.

• Department of Chemical Engineering and Technology, Indian Institute of Technology (BHU)

autor

Kumar A.

• Department of Botany, Banaras Hindu University, India

autor

Shukla P. N.

• Department of Botany, Banaras Hindu University, India

autor

Singh R.

• Department of Botany, Banaras Hindu University, India

autor

Singh P. K.

• Department of Botany, Banaras Hindu University, India

autor

Pandey K. D.

• Department of Botany, Banaras Hindu University, India

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