Antioxidative capacity of phenolic compounds extracted from Lolium perenne and Lolium arundinaceum infected with Neotyphodium (Hypocreales: Clavicipitaceae)

• Autorzy: Qawasmeh A. , Raman A. , Wheatley W. , Nicol H.
• Języki publikacji: EN

Abstrakty

EN

Members of Neotyphodium endophytic fungi infecting Lolium perenne L. and Lolium arundinaceum Darb. alter the synthesis of several metabolites. In this study we determined the antioxidative capacity of phenolic compounds from L. perenne and L. arundinaceum infected with Neotyphodium lolii (Latch, Christensen et Samuels) and Neotyphodium coenophialum (Morgan-Jones et Gams) Glenn, Bacon et Hanlin, respectively. The antioxidant capacity was determined by measuring the scavenging capacity of aqueous methanolic extracts to the free-radical DPPH (2,2-diphenyl-1-picrylhydrazyl). L. perenne infected with ‘wild-type’ strain endophyte showed the highest scavenging capacity, whereas endophyte-free showed the least. Those infected with the ‘novel’ strains AR1 and AR37 showed intermediate capacities. L. arundinaceum infected with the ‘novel’ strain AR542 showed a lower scavenging capacity compared with endophyte-free counterparts, regardless of the L. arundinaceum germplasm. The endophyte-free Mediterranean and Continental L. arundinaceum showed a higher capacity to scavenge DPPH when compared with the endophyte-infected Mediterranean and Continental L. arundinaceum. These results suggest that the endophytic fungi alter the antioxidative capacity of the grasses.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2012
• Tom: 34
• Numer: 2
• Opis fizyczny: p.827-833,fig.,ref.

Twórcy

autor

Qawasmeh A.

• School of Agricultural and Wine Sciences, Charles Sturt University, Orange, NSW 2800, Australia

autor

Raman A.

• School of Agricultural and Wine Sciences, Charles Sturt University, Orange, NSW 2800, Australia
• EH Graham Centre for Agricultural Innovation, Wagga Wagga, NSW 2678, Australia

autor

Wheatley W.

• School of Agricultural and Wine Sciences, Charles Sturt University, Orange, NSW 2800, Australia

autor

Nicol H.

• School of Agricultural and Wine Sciences, Charles Sturt University, Orange, NSW 2800, Australia

Bibliografia

• Ahmad P, Sarwat M, Sharma S (2008) Reactive oxygen species, antioxidants and signaling in plants. J Plant Biol 51:167–173
• Arora A, Sairam RK, Srivastava GC (2002) Oxidative stress and antioxidative system in plants. Curr Sci 82:1227–1238
• Assuero SG, Matthew C, Kemp P, Barker DJ, Mazzanti A (2002) Effects of water deficit on mediterranean and temperate cultivars of tall fescue. Aust J Agric Res 53:29–40
• Bidar G, Garcon G, Pruvot C, Dewaele D, Cazier F, Douay F, Shirali P (2007) Behaviour of Trifolium repens and Lolium perenne growing in a heavy metal contaminated field: plant metal concentration and phytotoxicity. Environ Poll 147:546–553
• Bonnet M, Camares O, Veisseire P (2000) Effects of zinc and influence of Acremonium lolii on growth parameters, chlorophyll a fluorescence and antioxidant enzyme activities of ryegrass (Lolium perenne L. cv Apollo). J Exp Bot 51:945–953
• Champagnat P, Heitz A, Carnat A, Fraisse D, Carnat AP, Lamaison JL (2008) Flavonoids from Vetiveria zizanioides and Vetiveria nigritana (Poaceae). Biochem Syst Ecol 36:68–70
• Grace S (2005) Phenolics as antioxidants. In: Smirnoff N (ed) Antioxidants and reactive oxygen species in plants. Blackwell, New York, pp 141–168
• Hahn H, Huth W, Schoberlein W, Diepenbrock W (2003) Detection of endophytic fungi in Festuca spp. by means of tissue immunoassay. Plant Breed 122:217–222
• Hoveland CS (1993) Importance and economic significance of the Acremonium endophytes to performance of animals and grass plant. Agric Ecosyst Environ 44:3–12
• Hume DE, Ryan DL, Cooper BM, Popay AJ (2007) Agronomic performance of AR37-infected ryegrass in northern New Zealand. Proc NZ Grassl Assoc 69:201–205
• Jiang YW, Huang BR (2001) Drought and heat stress injury to two cool-season turfgrasses in relation to antioxidant metabolism and lipid peroxidation. Crop Sci 41:436–442
• Malcata FX, Giao MS, Gonzalez-Sanjose ML, Rivero-Perez MD, Pereira CI, Pintado ME (2007) Infusions of Portuguese medicinal plants: dependence of final antioxidant capacity and phenol content on extraction features. J Sci Food Agric 87:2638–2647
• Malinowski DP, Belesky DP (2000) Adaptation of endophyteinfected cool-season grasses to environmental stresses: mechanisms of drought and mineral stress tolerance. Crop Sci 40:923–940
• Malinowski DP, Belesky DP (2006) Ecological importance of Neotyphodium spp. grass endophytes in agroecosystems. Grassl Sci 52:1–14
• Malinowski DP, Alloush GA, Belesky DP (1998) Evidence for chemical changes on the root surface of tall fescue in response to infection with the fungal endophyte Neotyphodium coenophialum. Plant Soil 205:1–12
• Malinowski DP, Kigel J, Pinchak WE (2009) Water deficit, heat tolerance, and persistence of summer-dormant grasses in the US southern plains. Crop Sci 49:2363–2370
• Michalska A, Ceglinska A, Amarowicz R, Piskula MK, Szawara–Nowak D, Zielinski H et al (2007) Antioxidant contents and antioxidative properties of traditional rye breads. J Agric Food Chem 55:734–740
• Obied HK, Bedgood DR, Prenzler PD, Robards K (2008) Effect of processing conditions, prestorage treatment, and storage conditions on the phenol content and antioxidant activity of olive mill waste. J Agric Food Chem 56:3925–3932
• Orozco-Cardenas M, Ryan CA (1999) Hydrogen peroxide is generated systemically in plant leaves by wounding and systemin via the octadecanoid pathway. P Natl Acad Sci 96:6553–6557
• Ponce MA, Bompadre MJ, Scervino JM, Ocampo JA, Chaneton EJ, Godeas AM (2009) Flavonoids benzoic acids and cinnamic acids isolated from shoots and roots of Italian rye grass (Lolium multiflorum Lam.) with and without endophyte association and arbuscular mycorrhizal fungus. Biochem Syst Ecol 37:245–253
• Popay AJ, Hume DE, Baltus JG, Latch GCM, Tapper BA, Lyons TB, Cooper BM, Pennell C, Erens JPJ, Marshall SL (1999) Ryegrass endophyte: an essential New Zealand symbiosis. In: Woodfield DR, Matthew C (eds) Proceedings of a New Zealand Grassland Association Symposium, New Zealand Grassland Association, Auckland pp 113–122
• Qawasmeh A, Bourke CA, Lee S, Gray M, Wheatley WM, Sucher NJ, Raman A (2011) GC–MS analysis of volatile secondary metabolites in ‘Mediterranean’ and ‘Continental’ Festuca arundinacea (Poaceae) infected with the fungal endophyte Neotyphodium coenophialum strain AR542. Acta Chromatogr 24:621–628
• Rasmussen S, Parsons AJ, Fraser K, Xue H, Newman JA (2008) Metabolic profiles of Lolium perenne are differentially affected by nitrogen supply, carbohydrate content, and fungal endophyte infection. Plant Physiol 146:1440–1453
• Salopek-Sondi B, Piljac-Žegarac J, Magnus V, Kopjar N (2010) Free radical scavenging activity and DNA damaging potential of auxins IAA and 2-methyl-IAA evaluated in human neutrophils by the alkaline comet assay. J Biochem Mol Toxicol 24:165–173
• Schulz B, Boyle C (2005) The endophytic continuum. Mycol Res 109:661–686
• Siegel MR, Latch GC, Johnson MC (1985) Acremonium fungal endophytes of tall fescue and perennial ryegrass: significance and control. Plant Dis 69:179–188
• Torres MS, White JF, Zhang X, Hinton DM, Bacon WC (2011) Endophyte-mediated adjustments in host morphology and physiology and effects on host fitness traits in grasses. Fungal Ecol. doi:10.1016/j.funeco.2011.05.006
• Treutter D (2006) Significance of flavonoids in plant resistance: a review. Environ Chem Lett 4:147–157
• van de Staaij J, de Bakker NVJ, Oosthoek A, Broekman R, van Beem A, Stroetenga M, Aerts R, Rozema J (2002) Flavonoid concentrations in three grass species and a sedge grown in the field and under controlled environment conditions in response to enhanced UV-B radiation. J Photochem Photobiol (B Biol) 66:21–29
• White JF, Torres MS (2010) Is plant endophyte-mediated defensive mutualism the result of oxidative stress protection? Physiol Plant 138(4):440–446

Uwagi

Rekord w opracowaniu