Molecular identification of Cryptosporidium parvum in rabbits (Oryctolagus cuniculus) in Nigeria

• Autorzy: Ayinmode A.B. , Agbajelola V.I.
• Języki publikacji: EN

Abstrakty

EN

Rabbits are commonly reared by households and farmers in Nigeria as a source of meat, but there is no information available on Cryptosporidium genotypes occurring in rabbits in Nigeria. Fecal samples were collected from 107 rabbits and examined by modified Ziehl-Neelsen technique for the presence of Cryptosporidium oocysts. An infection rate of 3.7% (4/107) was obtained and all microscopy-positive samples were genotyped and subtyped to determine the circulating Cryptosporidium species using sequence analysis of the 18S rRNA gene and 60-kDa glycoprotein (gp60) gene, respectively. All the four microscopy-positive samples were identified as C. parvum by 18S rRNA gene. However, analysis of the gp60 gene revealed the presence of C. parvum subtype IIc, which is commonly found in humans in two isolates. These findings indicate natural infection of rabbits with C. parvum and underscore the need to investigate the probable role of animal hosts in the epidemiology of Cryptosporidium infection. This is the first report on genetic characterization of Cryptosporidium infecting rabbits in Nigeria.

• Wydawca: -
• Czasopismo: Annals of Parasitology
• Rocznik: 2019
• Tom: 65
• Numer: 3
• Opis fizyczny: p.237-243,fig.,ref.

Twórcy

autor

Ayinmode A.B.

• Department of Veterinary Parasitology and Entomology, University of Ibadan, Ibadan, Nigeri

autor

Agbajelola V.I.

• Department of Veterinary Parasitology and Entomology, University of Ibadan, Ibadan, Nigeri

Bibliografia

• [1] Tyzzer E.E. 1912. Cryptosporidium parvum (sp.nov.), a coccidium found in the small intestine of the common mouse. Archiv für Protistenkunde 26: 394-412.
• [2] Inman L.R., Takeuchi L.R. 1979. Spontaneous cryptosporidiosis in an adult female rabbit, Veterinary Pathology 16: 89-95.
• [3] Xiao L., Sulaiman I.M., Ryan U.M., Zhou L., Atwill E.R., Tischler M.L., Zhang X., Fayer R., Lal A.A. 2002. Host adaptation and host-parasite co-evolution in Cryptosporidium: implications for taxonomy and public health, International Journal of Parasitology 32: 1773-1785.
• [4] Ryan U.M., Xiao L., Read C., Zhou L., Lal A.A., Pavlasek I. 2003. Identification of novel Cryptosporidium genotypes from the Czech Republic. Applied Environmental Microbiology 69: 4302-4307. doi:10.1128/AEM.69.7.4302-4307.2003
• [5] Robinson G., Wright Elwin S., K., Hadfield S.J., Katzer F., Bartley P.M., Hunter P.R., Nath M., Innes E.A., Chalmers R.M. 2010. Re-description of Cryptosporidium cuniculus Inman and Takeuchi, 1979 (Apicomplexa: Cryptosporidiidae): morphology, biology and phylogeny. International Journal of Parasitology 40: 539-1548. https://doi.org/10.1016/j.ijpara.2010.05.010
• [6] Robinson G., Elwin K., Chalmers R.M. 2008. Unusual Cryptosporidium genotypes in human cases of diarrhea. Emerging Infectious Diseases 14: 1800-1802. https://doi.org/10.3201/eid1411.080239
• [7] Chalmers R.M., Robinson G., Elwin K., Hadfield S.J., Xiao L., Ryan U.M., Modha D., Mallaghan C. 2009. Cryptosporidium rabbit genotype, a newly identified human pathogen. Emerging Infectious Diseases 15: 829-830. doi:10.3201/eid1505.081419
• [8] Puleston R.L., Mallaghan C.M., Modha D.E., Hunter P.R., Nguyen-Van-Tam J.S., Regan C.M., Nichols G.L., Chalmers R.M. 2014. The first recorded outbreak of cryptosporidiosis due to Cryptosporidium cuniculus (formerly rabbit genotype), following a water quality incident. Journal of Water and Health 12: 41-50. https://doi.org/10.2166/wh.2013.097
• [9] Koehler A.V., Whipp M.J., Haydo S.R., Gasser R.B. 2014. Cryptosporidium cuniculus - new records in human and kangaroo in Australia. Parasites and Vectors 7: 492. https://doi.org/10.1186/s13071-014-0492-8
• [10] Nolan M.J., Jex A.R., Haydon S.R., Stevens M.A., Gasser R.B. 2010. Molecular detection of Cryptosporidium cuniculus in rabbits in Australia, Infection, Genetics and Evolution 10:1179-1187. https://doi.org/10.1016/j.meegid.2010.07.020
• [11] Shi K., Jian F., Lv C., Ning C., Zhang L., Dearen T.K., Li N., Qi M., Xiao L. 2010. Prevalence, genetic characteristics and zoonotic potential of Cryptosporidium species causing infections in farm rabbits in China. Journal Clinical Microbiology 48: 3263-3266. https://doi.org/10.1128/JCM.00819-10
• [12] Zhang W., Shen Y., Wang R., Liu A., Ling H., Li Y., Cao J., Zhang X., Shu J., Zhang L. 2012. Cryptosporidium cuniculus and Giardia duodenalis in rabbits: genetic diversity and possible zoonotic transmission. PLoS One 7: e31262. doi:10.1371/journal.pone.0031262
• [13] Liu X., Zhou X., Zhong Z., Chen W., Deng J., Niu L., Wang Q. 2014. New subtype of Cryptosporidium cuniculus isolated from rabbits by sequencing the gp60 gene. Journal of Parasitology 100: 532-536. https://doi.org/10.1645/13-223.1
• [14] ANOFEL Cryptosporidium National Network. 2010. Cryptosporidium National Network Laboratory-based surveillance for Cryptosporidium in France: 2006-2009. Euro Surveillance 15: 19642-19690.
• [15] Molloy S.F., Smith H.V., Kirwan P., Nichols R.A., Asaolu S.O., Connelly L., Holland C.V. 2010. Identification of a high diversity of Cryptosporidium species genotypes and subtypes in a pediatric population in Nigeria. American Journal of Tropical Medicine and Hygiene 82: 608-613. https://doi.org/10.4269/ajtmh.2010.09-0624
• [16] Onifade A.A., Abu O.A., Obiyan R.I., Abanikannda O.T. 1999. Rabbit production in Nigeria: Some aspects of current status and promotional strategies. World Rabbit Science 7: 51-58. https://doi.org/10.4995/wrs.1999.380
• [17] Casemore D.P. 1991. Laboratory methods for diagnosing cryptosporidiosis. Journal of Clinical Pathology 44: 445-451. https://dx.doi.org/10.1136/jcp.44.6.445
• [18] Wee S.H., Lee C.G., Kim B.S., Joo H.D., Kang S.W. 1994. Isolation of Cryptosporidium parvum oocysts from fecal samples: the combination of ether extraction and discontinuous sucrose gradients. Korean Journal of Parasitology 32 : 7-12 (in Korean with summary in English),
• [19] Sulaiman I.M., Hira P.R., Zhou L., Al-Ali F.M., Al-Shelahi F.A., Shweiki H.M., Iqbal J., Khalid N., Xiao L. 2005. Unique endemicity of cryptosporidiosis in children in Kuwait. Journal of Clinical Microbiology 43: 2805-2809. doi:10.1128/JCM.43.6.2805-2809.2005
• [20] Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. 2013. MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Molecular Biology and Evolution 30: 2725-2729. https://doi.org/10.1093/molbev/mst197
• [21] Chalmers R.M. 1996. The distribution of Cryptosporidium in livestock and wild animal populations on a Warwickshire farm. PhD thesis, Coventry University, Coventry.
• [22] Kaupke A., Kwit E., Chalmers R.M., Michalski M.M., Rzezutka A. 2014. An outbreak of massive mortality among farm rabbits associated with Cryptosporidium infection. Research in Veterinary Science 97: 85-87. https://doi.org/10.1016/j.rvsc.2014.04.016
• [23] Yang Z., Zhao W., Shen Y., Zhang W., Shi Y., Ren G., Yang D., Ling H., Yang F., Liu A., Cao J. 2016. Subtyping of Cryptosporidium cuniculus and genotyping of Enterocytozoon bieneusi in rabbits in two farms in Heilongjiang Province, China. Parasite 23: 52. https://doi.org/10.1051/parasite/2016063
• [24] Xiao L., Feng Y. 2008. Zoonotic cryptosporidiosis. FEMS Immunology and Medical Microbiology 52: 309-323. https://doi.org/10.1111/j.1574-695X.2008.00377.x
• [25] Bouzid M., Tyler K.M., Christen R., Chalmers R.M., Elwin K., Hunter P.R. 2010. Multi-locus analysis of human infective Cryptosporidium species and subtypes using ten novel genetic loci. BMC Microbiology 10: 213. https://doi.org/10.1186/1471-2180-10-213
• [26] Dyachenko V., Kuhnert Y., Schmaeschke R., Etzold M., Pantchev N., Daugschies A. 2010. Occurrence and molecular characterization of Cryptosporidium sp. genotypes in European hedgehogs (Erinaceus europaeus). in Germany. Parasitology 137: 205-216. https://doi.org/10.1017/ S0031182009991089
• [27] Krawczyk A.I., Docters van Leeuwen D., Jacobs-Reitsma W., Wijnands L.M., Bouw E.I., Jahfari S., van Hoek H.A., van der Giessen W.B., Roelfsema J.H., Kroes M., Kleve J., Dullemont Y., Sprong H., de Bruin A. 2015. Presence of zoonotic agents in engorged ticks and hedgehog faeces from Erinaceus europaeus in (sub) urban areas. Parasites and Vectors 8: 210. https://doi.org/10.1186/s13071-015-0814-5
• [28] Robinson G., Chalmers R.M. 2010. The European rabbit (Oryctolagus cuniculus), a source of zoonotic cryptosporidiosis. Zoonoses and Public Health 57: e1-e13. doi:10.1111/j.1863-2378.2009.01308.x

Identyfikatory

DOI

10.17420/ap6503.205