Measurements of essential oil extract and antioxidants in Syrian Myrtus communis L. leaves using photochemiluminescence assay

• Autorzy: Zayzafoon G. , Odeh A. , Mahzia Y. , Allaf A. W.

Warianty tytułu

PL

Określenie zawartości olejku eterycznego i przeciwutleniaczy w mircie zwyczajnym (Myrtus communis L.) rosnącym w Syrii za pomocą metody fotochemoluminescencyjnej

• Języki publikacji: EN

Abstrakty

EN

The essential oil extracts and antioxidant measurements of Syrian Myrtus communis L. leaves as hydrophilic and hydrophobic existence species have been carried out. The plant leaves as a source of antioxidants was tested by the influence of its aqueous and essential oil extracts on the yield of PCL solution applying very sensitive and reliable method. By means of a PCL assay, it was possible to assess the total antioxidants capacity of hydrophilic and hydrophobic species existence in Syrian Myrtus communis L. leaves. It has been found that the integral antioxidant capacity value of Syrian Myrtus communis L. leaves was found in Kurdaha site which has a value of 465.67±1.18 TE/g DM. The following three main substances were found in the essential oil extracts: a-pinene, cineole and limonene.

PL

W mircie zwyczajnym (Myrtus communis L.), roślinie zawierającej związki hydrofilne i hydrofobowe występującej w Syrii, przeprowadzono badanie olejku eterycznego pod kątem obecności przeciwutleniaczy. Zawartość przeciwutleniaczy w liściach rośliny badano dla wyciągu wodnego i olejku eterycznego stosując czułą i godną zaufania metodę fotochemoluminescencji (PCL). Za pomocą PCL można było określić całkowitą zdolność przeciwutleniającą związków hydrofobowych i hydrofilnych liści mirtu zwyczajnego rosnącego w Syrii. Stwierdzono, że zmierzona aktywność przeciwutleniająca w liściach zebranych na stanowisku Kurdaha miała wartość 465.67±1.18 TE/g DM. W olejku eterycznym znaleziono trzy główne związki: a-pinen, cineol i limonen.

Słowa kluczowe

EN

measurement; essential oil extract; antioxidant; Myrtus communis; leaf; photochemiluminescence; ascorbic acid; Syria; medicinal plant

• Wydawca: -
• Czasopismo: Herba Polonica
• Rocznik: 2011
• Tom: 57
• Numer: 3
• Opis fizyczny: p.5-19,fig.,ref.

Twórcy

autor

Zayzafoon G.

• Department of Chemistry, Atomic Energy Commission of Syria, P.O.Box 6091, Damascus, Syria

autor

Odeh A.

autor

Mahzia Y.

autor

Allaf A. W.

Bibliografia

• 1. Miliauskas G, Venskutonis PR, Van Beek TA. Screening of radical scavenging activity of some medicinal and aromatic plant extracts. Food Chem 2004; 85:231-7.
• 2. Bahorunl T, Aumjaud E, Ramphul H, Maheshyywaree R, Luximon-Ramma A, Trotin F, Aruoma OI. Phenolic constituents and antioxidant capacities of crataegus monogyan (Hawthorn) callus extracts. Nahrung/ Food 2003, 47:191-8.
• 3. Yoo KM, Lee CH, Lee H, Moon B, Lee CY. Relative antioxidant and cytoprotective activities of common herbs. Food Chem 2008; 106:929-36.
• 4. Elfellah MS, Akhter MH, Khan MT. Anti-hyperglycaemic effect of an extract of Myrtus communis in streptozotocin-induced diabetes in mice. J Ethnopharmacol1984; 11:275-81.a
• 5. Lee JH, Park JC, Choi JS. The antioxidant activity of Ecklonia stolonifera. Arch Pharm Res 1996; 19:223-7.
• 6. Lee KW, Hur HJ, Lee HJ, Lee CY. Antiproliferative effects of dietary phenolic substances and hydrogen peroxide. J Agric Food Chem 2005; 53:1990-95.
• 7. Lee KW, Kim YJ, Lee HJ, Lee CY. Cocoa has more phenolic phytochemicals and a higher antioxidant capacity than teas and red wine. J Agric Food Chem 2003; 51:7292-5.
• 8. Lee KW, Lee HJ, Kang KS, Lee CY. Preventive effects of vitamin C on carcinogenesis. Lancet 2002; 359(9301):172.
• 9. Madsen HL, Bertelsen G. Spices as antioxidants. Trends Food Sci Technol 1995; 6:27-7.
• 10. Rakitzis ET. Reaction of thioureas with Folin-Ciocalteu reagent. Anal Chim Acta 1975; 78:495-7.
• 11. Capecka E, Mareczek A, Leja M. Antioxidant activity of fresh and dry herbs of some Lamiaceae species. Food Chem 2005; 93:223-6.
• 12. Geleijnse JM, Launer LJ, Hofman A, Pols HAP, Witteman JCM. Tea flavonoids may protect against atherosclerosis – The Rotterdam study. Arch Int Med 1999; 159:2170-4.
• 13. Kahkonen MP, Hopia AI, Vuorela HJ, Rauha JP, Pihlaja K, Kujala TS, Heinonen M. Antioxidant activity of plant extracts containing phenolic compounds. J Agric Food Chem 1999; 47:3954-62.
• 14. Kang KA, Lee KH, Chae S, Koh YS, Yoo BS, Kim JH, Ham YM, Baik JS, Lee NH, Hyun JW. Triphlorethol-A from Ecklonia cava protects V79-4 lung fibroblast against hydrogen peroxide induced cell damage. Free Rad Res 2005; 39: 883-92.
• 15. Khokhar S, Magnusdottir SGM. Total phenol, catechin, and caffeine contents of teas commonly consumed in the United Kingdom. J Agric Food Chem 2002; 50:565-70.
• 16. Besco E, Braccioli E, Vertuani S, Ziosi P, Brazzo F, Bruni R, Sacchetti G, Manfredini S. The use of photochemiluminescence for the measurements of integral antioxidant capacity of baobab products. Food Chem 2007; 102:1352-6.
• 17. Miller NJ, Diplock AT, Rice-Evans C, Davis MJ, Gopinathan V, Milner A. A novel methods for measuring antioxidant capacity and its application to monitoring the antioxidant status in the premature neonates. Clin Sci 1993; 84:407-12.
• 18. Sanchez-Moreno C. Review: methods used to evaluate the free radical scavenging activity in food and biological system. Food Sci Tech Inter 2002; 8:121-37.
• 19. Ghiselli A, Serafini M, Natella F, Scacccini C. Total antioxidant capacity as a tool to asses redox status: critical review an experimental data. Free Rad Biol Med 2000; 29:1106-14.
• 20. Cao G, Prior RL. Comparison of different analytical methods for assessing total antioxidant capacity of human serum. Clin Chem 1998; 44:1309-15.
• 21. Benzie IFF , Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant” power: the FRAP assay. Annal Biochem 1996; 239:70-76.
• 22. Sacchetti G, Muzzoli M, Statti GA, Conforti F, Bianchi A, Agrimonti C, Ballero M, Poli F. Intra-specific biodiversity of Italian myrtle (Myrtus communis) through chemical markers profile and biological ctivities of leaf methanolic extracts. Nat Prod Res 2007; 21:167-79.
• 23. Zayzafoon G, Odeh A, Ibrahim M, Allaf AW. Determination of integral antioxidants capacity in Syrian hawthorn fruits and flowers using photochemiluminescence assay. Herba Pol 2010, 56:47-58.
• 24. Popov I, Lewin G. Antioxidative homeostasis: Characterisation by means of chemiluminescent technique in methods in enzymology. In: L. Packer. Oxidants and antioxidants. Part B. Vol. 300:96-100. Academic ress1999.
• 25. Zielińska D, Szawara-Nowak D, Ornatowska A, Wiczkowski W. The use of cyclic voltammetry, photochemiluminescence and spectrophotometric methods for the measurement of the antioxidant capacity of buckwheat sprouts. J Agric Food Chem 2007, 55:9891-8.
• 26. Zielińska D, Wiczkowski W, Piskuła MK. Evaluation of photochemiluminescent, spectrophotometric and cyclic voltammetry methods for the measurement of the antioxidant capacity: the case of roots separated from buckwheat sprouts. Polish J Food Nutr Sci 2008, 58:65-72.
• 27. Zielińska D, Zieliński H. Antioxidant activity of flavone C-glucosides determined by updated analytical strategies. Food Chem 2011, 124:672-8.
• 28. Demo A, Petrakis C, Kefalas P, Boskou D. Nutrient antioxidants in some herbs and Mediterranean plant leaves. Food Res Int 1998, 31:351-4.
• 29. Mimica-Dukić N, Bugarin D, Grbović S, Mitić-Ćulafić D, Vuković-Gačić B, Orčić D, Jovin E, Couladis M. Essential oil of Myrtus communis L. as a potential antioxidant and antimutagenic agents. Molecules 2010; 15:2759-70.
• 30. Gardeli C, Vassiliki P, Athanasios M, Kibouris T, Komaitis M. Essential oil composition of Pistacia lentiscus L. and Myrtus communis L.: evaluation of antioxidant capacity of methanolic extracts. Food Chem 2008, 107:1120-30.
• 31. Romani A, Coinu R, Carta S, Pinelli P, Galardi C, Vincieri FF , Franoconi F. Evaluation of antioxidant effect of different extracts of Myrtus communis L. Free Rad Res 2004; 38:97-103.
• 32. Montoro P, Tuberoso CIG, Piacente S, Perrone A, De Feo V, Cabras VP, Pizza C. Stability and antioxidant activity of polyphenols in extracts of Myrtus communis L. berries used for the preparation of myrtleliqueur. J Pharm Biomed Anal 2006; 41:1614-19.