PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2013 | 35 | 12 |

Tytuł artykułu

Rapid in vitro multiplication and ex vitro establishment of Caribbean copper plant (Euphorbia cotinifolia L.): an important medicinal shrub

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
An efficient micropropagation protocol was developed for E. cotinifolia by utilizing mature nodal segments for axillary shoot proliferation. The nodal explants from a 2-year-old plant were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations (0.5, 2.5, 5.0, 7.5 and 10.0 μM) of 6-benzyladenine (BA), Kinetin and 2-isopentenyl adenine singly as well as in combination with α-naphthalene acetic acid (NAA) or Indole-3-butyric acid (IBA) (0.1, 0.5 and 1.0 μM). The highest regeneration frequency (92 %) with multiple shoots (13.0 ± 1.15) and shoot length (4.23 ± 0.14 cm) was achieved on MS medium supplemented with 5.0 μM BA and 0.1 μM NAA after 8 weeks of culture. Further experiments were performed to test the effects of medium type, medium strength, pH and subculture passages on shoot induction and proliferation. An enhancement in average number of shoots (16.6 ± 0.45) per explant was obtained after four subculture passages. Micro shoots exhibited in vitro rooting on half strength MS medium containing 2.5 μM Indole-3-butyric acid (IBA) after 4 weeks of culture. The in vitro raised healthy plantlets with well-developed roots and shoots were successfully acclimatized in plastic cups containing sterile soilrite for 8 weeks under culture room conditions (150 PPFD) prior to field transfer. Through the acclimatization period (0–56 days), photosynthetic pigments (Chlorophyll a, b and Carotenoid content) decreased during the initial 2 weeks followed by significant increase during the successive period (21–56 days) of acclimatization. At the same time, all the tested antioxidant enzymes (SOD, CAT, APX and GR) exhibited an increasing trend throughout the acclimatization period. The culture room acclimatized plantlets were successfully established in earthen pots containing garden soil in greenhouse with 70 % survival rate.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

35

Numer

12

Opis fizyczny

p.3391-3400,fig.,ref.

Twórcy

autor
  • Plant Biotechnology Laboratory, Department of Botany, Aligarh Muslim University, Aligarh 202 002, India
autor
  • Plant Biotechnology Laboratory, Department of Botany, Aligarh Muslim University, Aligarh 202 002, India
autor
  • Plant Biotechnology Laboratory, Department of Botany, Aligarh Muslim University, Aligarh 202 002, India
  • Department of Plant Production, College of Food and Agricultural Sciences, King Saud University, P.O. Box. 2460, Riyadh 11451, Saudi Arabia
autor
  • Department of Plant Production, College of Food and Agricultural Sciences, King Saud University, P.O. Box. 2460, Riyadh 11451, Saudi Arabia

Bibliografia

  • Aebi H (1984) Catalase in vitro. In: Packer L (ed) Methods in enzymology. Oxygen radicals in biological systems. Academic Press, Orlando, pp 121–126
  • Apel K, Hirt H (2004) Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Ann Rev Plant Biol 55:373–399
  • Bailey LH (1949) Manual of cultivated plants, revised edition. McMillan, New York, p 88
  • Betancur-Galvis LA, Morales GE, Forero JE, Roldan J (2002) Cytotoxic and antiviral activities of Colombian medicinal plant extracts of the Euphorbia genus. Mem Inst Oswal Cruz Rio de Jane 97:541–546
  • Bhatia P, Ashwath N (2005) Effect of medium pH on shoot regeneration from the cotyledonary explants of tomato. Biotechnol 4:7–10
  • Blanco-Molina M, Tron GC, Macho A, Lucena C, Calzado MA, Munoz E, Appendino G (2001) Ingenol esters induce apoptosis in jurkat cells through an AP-1 and NF-kappa B independent pathway. Chem Biol 8:767–778
  • Borkowska B (2001) Morphological and physiological characteristics of micropropagated strawberry plants rooted in vitro or ex vitro. Sci Hort 89:195–206
  • Bradford MM (1976) A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein dye-binding. Ann Biochem 72:248–254
  • Carvalho L, Santos P, Amancia S (2002) Effect of light intensity and CO2 concentration on growth and the acquisition of in vivo characteristics during acclimatization of grapevine regenerated in vitro. Vitis 41:1–6
  • De Oliveira Y, Pinto F, da Silva ALL, Guedes I, Biasi LA, Quoirin M (2010) An efficient protocol for micropropagation of Melaleuca alternifolia Cheel. In Vitro Cell Dev Biol Plant 46:192–197
  • Dhindsa RS, Plumb-Dhindsa P, Thorpe TA (1981) Leaf senescence: correlated with increased levels of membrane permeability and lipid peroxidation and decreased levels of superoxide dismutase and catalase. J Exp Bot 32:93–101
  • Faisal M, Anis M (2010) Effect of light irradiations on photosynthetic machinery and antioxidative enzymes during ex-vitro acclimatization of Tylophora indica plantlets. J Plant Int 5:21–27
  • Faisal M, Siddique I, Anis M (2006) An efficient plant regeneration system for Mucuna pruriens L. (DC.) using cotyledonary node explants. In Vitro Cell Dev Biol Plant 42:59–64
  • Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soya bean root cells. Exp Cell Res 50:151–158
  • Gautam VK, Nanda N, Gupta SC (1993) Development of shoots and roots in anther derived callus of Azadirachta indica A. Juss-A medicinal tree. Plant Cell Tis Org Cult 34:13–18
  • Gill SS, Tuteja N (2010) Reactive oxygen species and antioxidant machinery in biotic stress tolerance in crop plants. Plant Physiol Biochem 48:909–930
  • Hirota M, Ohigashi H, Oki Y, Koshimizu K (1980) New ingenol-esters as piscicidal constituents of Euphorbia continifolia L. Agric Biol Chem 44:1351–1356
  • Huang LC, Murashige T (1976) Plant tissue culture media: major constituents, their preparation and some applications. Tis Cul Ass Man 3:538–539
  • Husain MK, Anis M, Shahzad A (2008) In vitro propagation of a multipurpose leguminous tree (Pterocarpus marsupium roxb.) using nodal explants. Acta Physiol Plant 30:353–359
  • Jayalakshmi B, Raveesha KA, Amruthesh KN (2011) Phytochemical analysis and antibacterial activity of Euphorbia cotinifolia Linn. leaf extracts against phytopathogenic bacteria. J Pharm Res 4:3759–3762
  • Kadlecek P, Ticha I, Capkova V, Schafer C (1998) Acclimatization of micropropagated tobacco plantlets. In: Garab G (ed) Photosynthesis: mechanisms and effects, vol V. Kluwer, Dordrecht, pp 3853–3856
  • Karim MZ, Yokota S, Rahman MM, Eizawa J, Saito Y, Azad MAK, Ishiguri F, Iizuka K, Yoshizawa N (2007) Efficient adventitious shoot regeneration from root explant of Aralia elata seem. Int J Bot 3:390–393
  • Lloyd GB, McCown BH (1980) Commercially feasible micropropagation of mountain laurel (Kalmia latifolia) by use of shoot tip culture. Proc Int Plant Prop Soc 30:421–437
  • MacKinney G (1941) Absorption of light by chlorophyll solution. J Biol Chem 140:315–322
  • Mimura T, Shindo C, Kato M, Yokota E, Sakano K, Ashihara H, Shimmen T (2000) Regulation of cytoplasmic pH under extreme acid conditions in suspension cultured cells of Catharanthus roseus: a possible role of inorganic phosphate. Plant Cell Physiol 41:424–431
  • Mitrovic A, Janosevic D, Budimir S, Pristov JB (2012) Changes in antioxidative enzymes activities during Tacitus bellus direct shoot organogenesis. Biol Plant 56:357–361
  • Mortan JF (1962) Ornamental plants with toxic and/or irritant properties II. State Horticultural Society, Florida, pp 484–488
  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue culture. Physiol Plant 15:473–497
  • Nakano Y, Asada K (1981) Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplasts. Plant Cell Physiol 22:867–880
  • Noodezh HM, Moieni A, Baghizadeh A (2012) In vitro propagation of the damask rose (Rosa damascena Mill.). In Vitro Cell Dev Biol Plant 48:530–538
  • Pereira JP, de Souza CP, Mendes NM (1978) Molluscicidal properties of the Euphorbia cotinifolia L. Rev Bras Pesqui Med Bio 11:345–351
  • Perveen S, Varshney A, Anis M (2011) Influence of cytokinins, basal media and pH on adventitious shoot regeneration from excised root cultures of Albizia lebbeck L. J For Res 22:47–52
  • Perveen S, Anis M, Aref IM (2012) In vitro morphogenic response and metal accumulation in Albizia lebbeck (L.) cultures grown under metal stress. Eur J For Res 131:669–681
  • Perveen S, Anis M, Aref IM (2013) Lipid peroxidation, H2O2 content, and antioxidants during acclimatization of Abrus precatorius to ex vitro conditions. Biol Plant 57(3):417–424
  • Pinto G, Silva S, Loureiro J, Costa A, Dias MC, Araujo C, Neves L, Santos C (2011) Acclimatization of secondary somatic embryos derived plants of eucalyptus globules labill: an ultrastructural approach. Trees 25:292–383
  • Rao MV (1992) Cellular detoxifying mechanism determines age dependent injury in tropical plants exposed to SO2. J Plant Physiol 40:733–740
  • Shan H, Da-Xing S, Mi-Li W (2006) Forestry Department, College of Forestry and Horticulture, Sichuan Agricultural University, Yaan, Sichuan 625014, China; In vitro Culture and Plantlet Regeneration of Euphorbia cotinifolia Linn. Sp. Pl. [J]; Plant Physiology Communications; 01
  • Shan H, Daxing S, Mili W, Miaomiao M (2007) Somatic embryogenesis and plantlet regeneration from leaves of Euphorbia cotinifolia. Scientia Silvae Sinicae 43:134–137
  • Siddique I, Anis M (2009) Direct plant regeneration from nodal explants of Balanites aegyptiaca L. (Del.) a valuable medicinal tree. New For 37:53–62
  • Smirnoff N (2000) Ascorbic acid: metabolism and functions of a multi-facetted molecule. Curr Opin Plant Biol 3:229–235
  • Van Huylenbroek JM, Piqueras A, Debergh PC (2000) The evolution of photosynthetic capacity and the antioxidant enzymatic system during acclimatization of micropropagated Calathea plants. Plant Sci 155:59–66
  • Varshney A, Anis M (2012) Improvement of shoot morphogenesis in vitro and assessment of changes of the activity of antioxidant enzymes during acclimation of micropropagated plants of desert teak. Acta Physiol Plant 34:859–867
  • Warakagoda PS, Subasinghe S (2009) In vitro culture establishment and shoot proliferation of Jatropha curcas L. Trop Agri Res Exten 12:77–80
  • Xu J, Wang Y, Zhang Y, Chai T (2008) Rapid in vitro multiplication and ex vitro rooting of Malus zumi (Matsumura) Rehd. Acta Physiol Plant 30:129–132
  • Yan L (2009) Physiological responses of tomato seedlings (Lycopersicon esculentum) to salt stress. Mod App Sci 3:171–176

Uwagi

rekord w opracowaniu

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-eba3836b-4fd0-41f7-a254-100a00c9c5a7
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.