Identification of medicinal plant Schisandra chinensis using a potential DNA barcode ITS2

• Autorzy: Li X.-K. , Wang B. , Han R.-C. , Zheng Y.-C. , Yin H.-B. , Xu L. , Zhang J.-K. , Xu B.-L.
• Języki publikacji: EN

Abstrakty

EN

To test whether the internal transcribed spacer 2 (ITS2) region is an effective marker for using in authenticating of the Schisandra chinensis at the species and population levels, separately. And the results showed that the wild populations had higher percentage of individuals that had substitution of C→A at site 86-bp than the cultivated populations. At sites 10-bp, 37-bp, 42-bp and 235-bp, these bases of the Schisandra sphenanthera samples differed from that of S. chinensis. Two species showed higher levels of inter-specific divergence than intra-specific divergence within ITS2 sequences. However, 24 populations did not demonstrate much difference as inter-specific and intra-specific divergences were concerned. Both S. chinensis and S. sphenanthera showed monophyly at species level, yet the samples of different populations shown polyphyly at population level. ITS2 performed well when using BLAST1 method. ITS2 obtained 100% identification success rates at the species level for S. chinensis, with no ambiguous identification at the genus level for ITS2 alone. The ITS2 region could be used to identify S. chinensis and S. sphenanthera in the “Chinese Pharmacopoeia”. And it could also correctly distinguish 100% of species and 100% of genera from the 193 sequences of S. chinensis. Hence, the ITS2 is a powerful and efficient tool for species identification of S. chinensis.

Słowa kluczowe

EN

identification; internal transcribed spacer; medicinal plant; Schisandra chinensis; DNA barcode

• Wydawca: -
• Czasopismo: Acta Societatis Botanicorum Poloniae
• Rocznik: 2013
• Tom: 82
• Numer: 4
• Opis fizyczny: p.283-288,fig.,ref.

Twórcy

autor

Li X.-K.

• Pharmacy College, Liaoning University of Traditional Chinese Medicine, China

autor

Wang B.

• Pharmacy College, Liaoning University of Traditional Chinese Medicine, China

autor

Han R.-C.

• Pharmacy College, Liaoning University of Traditional Chinese Medicine, China

autor

Zheng Y.-C.

• Pharmacy College, Liaoning University of Traditional Chinese Medicine, China

autor

Yin H.-B.

• Pharmacy College, Liaoning University of Traditional Chinese Medicine, China

autor

Xu L.

• Pharmacy College, Liaoning University of Traditional Chinese Medicine, China

autor

Zhang J.-K.

• Pharmacy College, Liaoning University of Traditional Chinese Medicine, China

autor

Xu B.-L.

• Pharmacy College, Liaoning University of Traditional Chinese Medicine, China

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Identyfikatory

DOI

10.5586/asbp.2013.032