Attenuation of duck hepatitis virus and evaluation of its usefulness for duckling immunisation. I. Studies on attenuation of the virus

• Autorzy: Gazdzinski P.
• Języki publikacji: EN

Abstrakty

EN

A strain of duck hepatitis virus (DHV) isolated from a field case of duck virus hepatitis (DVH) was passaged 50 times in chicken embryos. Influence of serial passages on EID₅₀ and ELD₅₀ titers, virus concentration in the amnioallantoic fluid and in embryo with chorioallantoic membrane, pathogenic properties of the selected virus passages for ducklings have been determined. It has been shown that with the increased number of passages both titers were becoming higher. EID₅₀ was higher than ELD₅₀ up to the 36th passage, and thereafter they remained at the same level. In each examined passage, the concentration of DHV in embryo and chorioallantoic membrane was higher when compared with amnioallantoic fluid. Between the 30th and the 40th virus passages, DHV appeared to be apathogenic for one-day-old ducklings. Histopathological examinations of the liver sections of ducklings inoculated with the attenuated strain did not show lesions characteristic of DVH.

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 1979
• Tom: 23
• Numer: 3-4
• Opis fizyczny: p.80-89,fig.,ref.

Twórcy

autor

Gazdzinski P.

• Laboratory of Water Fowl Diseases, The Veterinary Research Institute, Pulawy, Poland

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