Comparison of isozyme, RAPD and AFLP markers in genetic similarity assessment of CMS ogura F1 hybrids of winter oilseed rape (Brassica napus L.) parental lines

• Autorzy: Liersch A. , Bocianowski J. , Kozak M. , Bartkowiak-Broda I.
• Języki publikacji: EN

Abstrakty

EN

Isozyme, RAPD and AFLP markers were evaluated and compared for their ability to determine genetic similarity in a set of 18 parental lines of winter oilseed rape F1 hybrids developed using CMS ogura. Five isozyme systems, 64 RAPD starters and 23 EcoRI+3/MseI+3 AFLP primer combinations generated 597 polymorphic markers. These polymorphic fragments were chosen for estimation of genetic similarity. Of the total number of polymorphic products, polymorphic zymograms constituted only 3.0% of the markers, 57 RAPD primers 37.7%, and 23 AFLP primer combinations 59.3%. The size of RAPD polymorphic products ranged from 564 to 2100 bp. On average there were four amplified bands per primer, with 61.0% polymorphism. The AFLP polymorphic fragments ranged from 72 to 1352 bp in size. AFLP assays generated 15 bands per primer pair on average and detected roughly four times more bands than with RAPD analysis. The genetic similarity coefficients based on all marker data range from 0.52 to 0.84. The correlation of genetic similarities based on RAPD and AFLP markers was 0.58. Estimated genetic similarity based on isozyme data was not correlated with genetic similarity derived from the two DNA-based markers. The dendrogram constructed with the three types of markers taken together grouped all the winter oilseed rape parental lines into several similar clusters. The genomic distribution and frequency of the RAPD and AFLP markers can serve well as estimators of genetic similarity between parental lines of F1 CMS ogura hybrids.

Słowa kluczowe

EN

Brassica napus; winter oilseed rape; genetic similarity; F1 hybrid; CMS line; AFLP method; molecular marker; parental line

• Wydawca: -
• Czasopismo: Acta Biologica Cracoviensia. Series Botanica
• Rocznik: 2013
• Tom: 55
• Numer: 1
• Opis fizyczny: p.49-57,fig.,ref.

Twórcy

autor

Liersch A.

• Department of Oilseed Crops, Plant Breeding and Acclimatization Institute, National Research Institute, Strzeszynska 36, 60-479 Poznan, Poland

autor

Bocianowski J.

• Department of Mathematical and Statistical Methods, Poznan University of Life Sciences, Wojska Polskiego 28, 60-637 Poznan, Poland

autor

Kozak M.

• Department of Botany, Warsaw University of Life Sciences, Nowoursynowska 159, 02-776 Warsaw, Poland

autor

Bartkowiak-Broda I.

• Department of Oilseed Crops, Plant Breeding and Acclimatization Institute, National Research Institute, Strzeszynska 36, 60-479 Poznan, Poland

Bibliografia

• BASUNANDA P, RADOEV M, ECKE W, FRIEDT W, BECKER HC, and SNOWDON RJ. 2010. Comparative mapping of quantitativetrait loci involved in heterosis for seedling and yieldtraits in oilseed rape (Brassica napus L.). Theoreticaland Applied Genetics 120: 271–281.
• BECKER H, and ENGQVIST GM. 1995. The potential of resynthesized rapeseed for hybrid breeding. Proceedings of 9th International Rapeseed Congress 4–7 July 1995, vol. 1, 118–121. Cambridge, UK.
• BOCIANOWSKI J, KOZAK M, LIERSCH A, and BARTKOWIAK-BRODA I. 2011. A heuristic method of searching for interestingmarkers in terms of quantitative traits. Euphytica 181:89–100.
• BOCIANOWSKI J, and SEIDLER-ŁOżYKOWSKA K. 2012. The relationship between RAPD markers and quantitative traitsof caraway (Carum carvi L.). Industrial and CropsProduction 36: 135–139.
• CORBELLINI M, PERENZIN M, ACCERBI M, VACCINO P, and BORGHI B. 2002. Genetic diversity in bread wheat, asrevealed by coefficient of parentage and molecular markers,and its relationship to hybrid performance.Euphytica 123: 273–285.
• CHEN W, ZHANG Y, YAO J, MA C, TU J, and TINGDONG F. 2011. Quantitative trait of loci mapping for two seed yield component traits in an oilseed rape (Brassica napus) cross. Plant Breeding 130: 640–646.
• ĆWIKLIŃSKA A, BRODA Z, BOCIANOWSKI J, and DOBRZYCKA A. 2010. The usefulness of RAPD and AFLP markers for determining genetic similarity in rye (Secale L.) species and subspecies. Acta Biologica Cracoviensia Series Botanica 52(1): 19–25.
• DAS S, RAJAGOPAL J, BHATIA S, SRIVASTAVA PS, and LAKSHMIKUMARAN M. 1999. Assessment of genetic variationwithin Brassica campestris cultivars using amplifiedfragment length polymorphism and random amplificationof polymorphic DNA markers. Journal ofBiosciences 24/2: 433–440.
• DIERS BW, MC VETTY PBE, and OSBORN TC. 1996. Relationship between heterosis and genetic distance based on restrictionfragment length polymorphism markers in oilseedrape (Brassica napus L.). Crop Science 36: 79–83.
• DOS SANTOS JB, NIENHUIS P, SKROCH J, TIVANG J, and SLOCUM MK. 1994. Comparison of RAPD and RFLPgenetics markers in determining genetic similarityamong Brassica oleracea L. genotypes. Theoretical andApplied Genetics 87: 909–915.
• DOYLE JJ, and DOYLE JL. 1990. Isolation of plant DNA from fresh tissue. Focus 12: 13–15.
• FALCONER DS, and MACKAY TFC. 1996. Introduction to Quantitative Genetics. 4th ed. Longman, England. FOISSET N, DELOURME R, BARRET P, HUBERT N, LandRY BS, and RENARD M. 1996. Molecular-mapping analysis in Brassica napus using isozyme, RAPD and RFLP markers on a doubled-haploid progeny. Theoretical and Applied Genetics 93: 1017–1025.
• HASAN M, SEYIS F, BADANI AG, PONS-KÜHNEMANN J, LÜHS W, FRIEDT W, and SNOWDON RJ. 2005. Surveying geneticdiversity in the Brassica napus gene pool using SSRmarkers. Genetic Resources and Crop Evolution 53:793–802.
• HASAN M, FRIEDT W, FREITAG NM, LINK K, PONS-KÜHNEMANN J, and SNOWDON RJ. 2008. Association of gene-linked SSR to seed glucosinolates content in oilseed rape (Brassica napus ssp. napus). Theoretical and Applied Genetics 116: 1035–1049.
• HU S, YU CH, ZHAO H, SUN G, ZHAO S, VYVADILOVA M, and KUCERA V. 2007. Genetic diversity of Brassica napus L.germplasm from China and Europe assessed by someagronomically important characters. Euphytica 154:9–16.
• HUNTER RL, and MARKERT CL. 1957. Histochemical demonstration of enzymes separated by zone electrophoresis in starch gels. Science 125: 1294–1295.
• JAVIDFAR F, RIPLEY VL, ROSLINSKY V, ZEINALI H, and ABDMISHANI C. 2006. Identification of molecular markers associated with oleic and linolenic acid in spring oilseed rape (Brassica napus). Plant Breeding 125: 65–71.
• KOZAK M, BOCIANOWSKI J, LIERSCH A, TARTANUS M, BARTKOWIAKBRODA I, PIOTTO F, and AZEVEDO RA. 2011. Genetic divergence is not the same as phenotypic divergence. Molecular Breeding 28(2): 277–280.
• KRAJEWSKI P, BOCIANOWSKI J, GAWŁOWSKA M, KACZMAREK Z, PNIEWSKI T, ŚWIĘCICKI W, and WOLKO B. 2012. QTL for yield components and protein content: a multienviron ment study of two pea (Pisum sativum L.) populations. Euphytica 183: 323–336.
• KRZYMIŃSKA A, GAWŁOWSKA M, WOLKO B, and BOCIANOWSKI J. 2008. Genetic diversity of ornamental Allium species andcultivars assessed with isozymes. Journal of AppliedGenetics 49(3): 213–220.
• LEFORT-BUSON M, GUILLOT-LEMOINE B, and BARTKOWIAK-BRODA I. 1987. A Brassica study of rapeseed (Brassica napus) F1 hybrids from lines of same and different geographic origins.Proceeding of the 7th International RapeseedCongress 11–14 May 1987: 63–68. Poznan, Poland.
• LEFORT-BUSON M, HEBERT Y, and DAMERVAL C. 1988. Les outils d' évaluation de la divérsité génétique et phénotypique.Agronomie 8(3): 173–178.
• LIERSCH A. 2005. Influence of genetic variability on heterosis effect in winter oilseed rape (Brassica napus L.). Ph.D. dissertation, Plant Breeding and AcclimatizationInstitute, Poznan, Poland [in Polish].
• LOMBARD V, BARIL CP, DUBREUIL P, BLOUET F, and ZHANG D. 2000. Genetic relationships and fingerprinting of rapeseed cultivars by AFLP. Crop Science 40: 1417–1425.
• LOMBARD V, and DELOURME R. 2001. A consensus linkage map for rapeseed (Brassica napus L.): construction and integrationof three individual maps from DH populations.Theoretical and Applied Genetics 103: 491–507.
• MOHAMMADI SA, and PRASANNA BM. 2003. Analysis of genetic diversity in crop plants-salient statistical tools and considerations.Crop Science 43: 1235–1248.
• MOLL RH, LOONQUIST JH, VELEZ FORTUNO J, and JOHNSON EC. 1965. The relationship of heterosis and genetics divergencein maize. Genetics 52: 139–144.
• NAKAJIMA Y, OEDA K, and YAMAMOTO T. 1998. Characterization of genetic diversity of nuclear and mitochondrialgenomes in Daucus varieties by RAPD and AFLP. PlantCell Reports 17: 848–853.
• NEI M, and LI W. 1979. Mathematical model for studying genetic variation in terms of restriction endonucleases.Proceedings of the National Academy of Sciences of theUSA 76: 5269–5273.
• OFORI A, BECKER HC, and KOPISH-OBUCH FJ. 2008. Effect of crop improvement on genetic diversity in oilseedBrassica rapa (turnip-rape) cultivars, detected by SSRmarkers. Journal of Applied Genetics 49: 207–212.
• OUJI A, SUSO MJ, ROUAISSI M, ABDELLAOUI R, and GAZZAH ME. 2011. Genetic diversity of nine faba bean (Vicia faba L.)populations revealed by isozyme markers. Genes &Genomics 33: 31–38.
• PAYNE R, MURREY D, HARDING S, BAIRD D, SOUTOU D, and LANE P. 2003. GenStat for Windows (7th edition) –Introduction. VSN International, Oxford, England.
• SCHRAG TA, MÖHRING J, MAURER HP, DHILLON BS, MELCHINGER AE, PIEPHO HP, SORENSEN AP, and FRISCH M. 2009. Molecular marker-based prediction of hybrid performancein maize using unbalanced data from multipleexperiments with factorial crosses. Theoretical andApplied Genetics 118: 741–751.
• SEYIS F, SNOWDON RJ, LÜHS W, and FRIEDT W. 2003. Molecular characterization of novel resynthesized rapeseed(Brassica napus) lines and analysis of their geneticdiversity in comparison with spring rapeseed cultivars.Plant Breeding 122: 473–478.
• SHEN J, LU G, FU T, and YANG G. 2003. Relationship between heterosis and genetic distance based on AFLPs inBrassica napus. Proceedings of the 11th InternationalRapeseed Congress 6–10 July 2003, vol. 1, 342–346.Copenhagen, Denmark.
• SHIELDS CR, ORTON CJ, and STUBER CW. 1983. An outline of general resource needs and procedures for the electrophoreticseparation of active enzymes from plant tissue.Isozymes. In: Tanksley SD, Orton TJ [eds.],Isozymes in Plant Genetics and Breeding, Part A,443–458. Elsevier Science Publishers, B.V, Amsterdam.
• SIMONIUC D, UPTMOOR R, FRIEDT W, and ORDON F. 2002. Genetic diversity and relationships among pea cultivarsrevealed by RAPDs and AFLPs. Plant Breeding 121:429–435.
• SNOWDON RJ, and FRIEDT W. 2004. Molecular markers in Brassica oilseed breeding: current status and future possibilities.Plant Breeding 123: 1–8.
• THORMANN CE, FERREIRA ME, CAMARGO LEA, TIVANG JG, and OSBORN TC. 1994. Comparison of RAPD and RFLPmarkers for estimating genetic relationships within andamong cruciferous species. Theoretical and AppliedGenetics 88: 973–980.
• VALLEJOS CE. 1983. Enzyme activity staining. In: Tanksley SD, Orton TJ [eds.], Isozymes in Plant Genetics and Breeding, Part A, 469–516. Elsevier Science Publishers, B.V, Amsterdam. VOS P, HOGERS R, BLEEKER M, REIJANS M, VAN DE LEE T,
• HORNES M, FRIJTERS A, POT J, TELEMAN J, KUIPER M, and ZABEAU M. 1995. AFLP: A new technique for DNA fingerprinting.Nucleic Acids Research 23: 4407–4414.
• WILLIAMS JGK, KUBELIK AR, LIVAK KJ, RAFALSKI JA, and TINGEY SV. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research 18: 6531–6535.
• WEEDEN NF. 1989. Applications of isozymes in plant breeding. Plant Breeding Reviews 6: 11–54.
• WEI LB, ZHANG HY, ZHENG YZ, MIAO HM, ZHANG TZ, and GUO WZ. 2009. A genetic linkage map construction forsesame (Sesamum indicum L.). Genes & Genomics 31:199–208.
• YU CY, HU SW, ZHAO HX, GUO AG, and SUN GL. 2005. Genetic distance revealed by morphological characters,isozymes, proteins and RAPD markers and their relationshipswith hybrid performance in oilseed rape(Brassica napus L.). Theoretical and Applied Genetics110: 511–518.
• ZHAO W, LEE GA, KWON SW, MA KH, LEE MC, and PARK YJ. 2012. Development and use of novel SSR markers formolecular genetic diversity in Italian Millet (Setaria italicaL.). Genes & Genomics 34: 51–57

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