Cloning and molecular characterization of cDNAs encoding three Ancylostoma ceylanicum secreted proteins

• Autorzy: Siwinska A.M. , Baska P. , Danilowicz-Luebert E. , Januszkiewicz K. , Dlugosz E. , Wedrychowicz H. , Cappello M. , Wisniewski M.
• Języki publikacji: EN

Abstrakty

EN

Ancylostoma ceylanicum belongs to a group of soil-transmitted helminths, which infect almost 576 mln people worldwide and are a major cause of anaemia and malnutrition. Upon contact with a permissive host, third-stage larvae (L3) residing in the environment become activated larvae (ssL3), a process associated with changes in the profile of gene expression. Ancylostoma secreted proteins (ASPs) are the major proteins secreted during larvae activation and play a crucial role in hookworm adaptation to parasitism. Here we report the cloning using RACE-PCR technique of three novel ASPs from the hookworm A. ceylanicum (Ace-asp-3, Ace-asp-4, and Ace-asp-5) and computational analysis of the protein sequences. All three proteins contain SCP (Sperm Coating Protein) domain characteristic for previously described ASP proteins. Real-time PCR analysis shows significant up-regulation of Ace-asp-3 and Ace-asp-5 expression in adult worms and correlated down-regulation in ssL3 larvae. On the other hand, expression of Ace-asp-4 was increased in ssL3 stages and decreased in adult parasites.

Słowa kluczowe

EN

molecular characteristics; cloning; cDNA encoding; Ancylostoma ceylanicum; protein; infection; secreted protein; hookworm; parasitism; real-time polymerase chain reaction

• Wydawca: -
• Czasopismo: Acta Parasitologica
• Rocznik: 2013
• Tom: 58
• Numer: 1
• Opis fizyczny: p.112-118,fig.,ref.

Twórcy

autor

Siwinska A.M.

• Division of Parasitology and Parasitic Diseases, Department Preclinical Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences-SGGW, 8 Ciszewskiego Street, 02-786 Warsaw, Poland

autor

Baska P.

autor

Danilowicz-Luebert E.

autor

Januszkiewicz K.

autor

Dlugosz E.

autor

Wedrychowicz H.

autor

Cappello M.

autor

Wisniewski M.

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