Factors altering the membrane fluidity of spinach thylakoid as determined by fluorescence polarization

• Autorzy: Lin Z.F. , Liu N. , Lin G. Z. , Peng C. L.
• Języki publikacji: EN

Abstrakty

EN

Alterations in fluidity of thylakoid membranes isolated from spinach chloroplasts in response to sodium bisulfite (NaHSO₃), hydrogen peroxide (H₂O₂), sodium dodecyl sulfate (SDS), bovine serum albumin (BSA), and free linoleic acid (LA) were investigated by means of a fluorescence polarization study with 1,6-diphenyl-1,3,5- hexatriene as the fluorescence probe. A decrease in fluidity and an increase in microviscosity of membrane were caused by NaHSO₃ and H₂O₂ treatment. In contrast, SDS and BSA were found to increase thylakoid membranes fluidity and decrease microviscosity, in which the corresponding correlation coefficients were -0.9995 to -0.9516 (SDS) and -0.9359 (BSA), respectively. No changes in thylakoid membranes fluidity induced by free LA were found until its concentration above 5 mM where the polarization value (P value) declined (increased fluidity). The results suggest that the changes in thylakoids membrane fluidity might depend on the characteristics, mechanism and extent of the interactions between membrane components and compounds added.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2011
• Tom: 33
• Numer: 3
• Opis fizyczny: p.1019-1024,fig.,ref.

Twórcy

autor

Lin Z.F.

• Key Laboratory of Vegetation Restoration and Management of Degraded Ecosystems, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510520, China

autor

Liu N.

• Key Laboratory of Vegetation Restoration and Management of Degraded Ecosystems, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510520, China

autor

Lin G. Z.

• Key Laboratory of Vegetation Restoration and Management of Degraded Ecosystems, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510520, China

autor

Peng C. L.

• College of Life Science, South China Normal University, Guangzhou 510631, China

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