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2012 | 56 | 2 |

Tytuł artykułu

Expression of progesterone receptors in hippocampal neurons in rabbit males

Autorzy

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
The aim of this study was to investigate the presence of progesterone receptors in hippocampal neurons of male rabbits and the distribution of these receptors in different areas of the hippocampus. The examinations were carried out on brains of five males, New Zealand breed, age of 2 years, weighing 2-3 kg. Immunohistochemical detection of receptors was performed using DAKO LSAB + Kit Peroxidase method. The sections were incubated with priman monoclonal antibody for progesterone receptor NCL-L- PGR-AB. The obtained results have shown the presence of progesterone receptors in neurons of CAI, CA3, and CA4 regions of the hippocampus. The reaction also occurred in the region of granular cells of the dental gyrus. The reaction was seen in the cytoplasm and nuclei of neurons simultaneously, whereas some neurons did not show any reaction in the nucleus and cytoplasm. Occurrence of progesterone receptors in most neurons of the hippocampus confirms a direct influence of progesterone on the hippocampus and its functions in rabbit males.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

56

Numer

2

Opis fizyczny

p.231-234,fig.,ref.

Twórcy

autor
  • Department of Animal Anatomy and Histology,University of Life Sciences, 20-950 Lublin, Poland
autor

Bibliografia

  • 1. Bonsall R.W., Rees H.D., Michael R.P.: Uptake of medroxyprogesterone acetate by progestin and androgen target neurons in the brain and pituitary gland of male cynomolgus monkeys. Neuroendocrinology 1990, 52, 573-580.
  • 2. Brown T.J., Clark A.S., Mac Lusky N.J.: Regional sex differences in progestin receptor induction in the rat hypothalamus: Effects of various doses of estradiol benzoate. J Neurosci 1987, 7, 2529-2536.
  • 3. Brown T.J., MacLusky N.J.: Progesterone modulation of estrogen receptors in microdissected regions of the rat hypothalamus. Mol Cell Neurosci 1994, 5, 283-290.
  • 4. Caba M., Rovirosa M.J., Beyer C., Gonzalez-Mariscal G.: Immunocytochemical detection of progesterone receptor in the female rabbit forebrain: distribution and regulation by oestradiol and progesterone. Neuroendocrinology 2003, 15, 855-64.
  • 5. Caba M., Beyer C., Gonzales-Mariscal G., Morrel J.: Immunocytochemical detection of estrogen receptor-α in the female rabbit forebrain: topography and regulation by estradiol.
  • 6. Camacho-Arroyo I., Mendoza-Rodriguez C.A., Mendoza- Garces L., Vanoye-Carlo A., Cerbon M.: Progesterone receptor mRNA expression and distribution in the female rabbit brain. J Steroid Biochem Mol Biol 2007, 104, 100- 104.
  • 7. Cooke B.M.., Tabibnia G., Breedlove S.M.: A brain sexual dimorphism controlled by adult circulating androgens. Proc Natl Acad Sci USA 1999, 96, 7538- 7540.
  • 8. Girgis M., Shih-Chang W.: Stereotaxic Atlas of the Rabbit Brain. Ed. W.H. Green, St.Louis, USA, 1981.
  • 9. Gordon G.G., Southren A.L., Tochimoto S., Olivo J., Altman K., Rand J., Lemberger L.: Effect of medroxyprogesterone acetate (Provera) on the metabolism and biological activity of testosterone. J Clin Endocrinol Metab 1970, 30, 449-456.
  • 10. Greco B., Allegreto E.A., Tetel M.J., Blaustein J.D.: Coexpression of ERß with ERα and progestin receptor proteins in the female rat forebrain: effects of estradiol treatment. Neuroendocrinologv 2001, 142, 5172-5181.
  • 11. Guerra-Araiza C., Reyna-Neyra A., Salazar A.M., Cerbon M.A., Morimoto S., Camacho-Arroyo I.: Progesterone receptor isoforms expression in the prepuberal and adult male rat brain. Brain Res Bull 2001, 54, 13-17.
  • 12. Hansen S., Sodersten P.: Reversal of progesterone inhibition of sexual behaviour in ovariectomized rats by high doses of progesterone. J Endocrinol 1979, 80, 381- 388.
  • 13. Hull E.M.: Effects of neonatal exposure to progesterone on sexual behavior of male and female rats. Physiol Behav 1981, 26, 401-405.
  • 14. McEwen B.S., Lieberburg I., Chaptal C., Davis P.G., Krey L.C., MacLusky N.J., Roy E.J.: Attenuating the defeminization of the neonatal rat brain: mechanisms of action of cyproterone acetate, l,4,6-androstatiene-3,17,- dione and synthetic progestin, R5020. Horm Behav 1979, 13, 269-281.
  • 15. Phelps S.M., Lydon J.P., O'Malley B.W., Crews D.: Regulation of male sexual behavior by progesterone receptor, sexual experience and androgen. Horm Behav 1998, 34, 294-302.
  • 16. Qadros P.S., Goldstein, A.Y.N., De Vries G.J., Wagner C.K.: Regulation of sex differences in progesterone receptor expression in the medial preoptic nucleus of postnatal rats. J Neuroendocrinol 2002, 14, 1-12.
  • 17. Quadros P.S., Pfau J.L., Goldstein A.Y.N., De Vries G.J., Wagner C.K.: Sex differences in progesterone receptor expression: A potential mechanism for estradiol-mediated sexual differentiation. Endocrinology 2002, 143, 3727- 3739.
  • 18. Quadros P.S., Lopez V., De Vries G.J., Chung W.C.J., Wagner C.K.: Progesterone receptors and the sexual differentiation of the medial preoptic nucleus. J Neurobiol 2002, 51, 24-32.
  • 19. Quadros, P.S., Wagner C.K.: Fetal progesterone is of maternal origin. Soc Neurosci Abstr 1999, 25, 228.
  • 20. Quadros P.S., Pfau J.L.,Wagner C.K.: Distribution of progesterone receptor immunoreactivity in the fetal and neonatal rat forebrain. J Comp Neurol 2007, 504, 42-56.
  • 21. Rhees R.W., Shryne J.E., Gorski R.A.: Onset of the hormone-sensitive perinatal period for sexual differentiation of the sexually dimorphic nucleus of the preoptic area in females. J Neurobiol 1990, 21, 781-786.
  • 22. Wagner C.K.: The many faces of progesterone: A role in adult and developing brain. Frontiers in Neuroendocrinology 2006, 27, 340-359.

Typ dokumentu

Bibliografia

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