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2013 | 16 | 3 |

Tytuł artykułu

Assessing the possibility of genetically modified DNA transfer from GM feed to broiler, laying hen, pig and calf tissues

Treść / Zawartość

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
The aim of this study was to assess the possibility of genetically modified DNA transfer from feed containing RR soybean or/and MON810 maize to animal tissues, gut bacterial flora, food of animal origin, and the fate of GM DNA in the animal digestive tract. The experiment was carried out on broilers, laying hens, pigs and calves. All animals were divided into four groups: I – control group (non-modified feed), II – GM soybean group (non-modified maize, RR soybean), III – GM maize group (MON810 maize, non-modified soybean), and IV – GM maize and soybean group (MON810 maize, RR soybean). Samples of blood, organs, tissues, digesta from the gastrointestinal tract, and eggs were analysed for the presence of plant species specific genes, and transgenic sequences of CaMV 35S promoter and NOS terminator. PCR amplifications of these GM sequences were conducted to investigate the GM DNA transfer from feed to animal tissues and bacterial gut flora. In none of the analysed samples of blood, organs, tissues, eggs, excreta and bacterial DNA were plant reference genes or GM DNA found. A GM crop diet did not affect bacterial gut flora as regards diversity of bacteria species, quantity of particular bacteria species in the animal gut, or incorporation of transgenic DNA to the bacteria genome. It can be concluded that MON810 maize and RR soybean used for animal feeding are substantially equivalent to their conventional counterparts. Genetically modified DNA from MON810 maize and RR soybean is digested in the same way as plant DNA, with no probability of its transfer to animal tissues or gut bacterial flora.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

16

Numer

3

Opis fizyczny

p.435-441,ref.

Twórcy

autor
  • Department of Hygiene of Animal Feedingstuffs, National Veterinary Research Institute, Al. Partyzantów 57, 24-100 Puławy, Poland
autor
  • Department of Hygiene of Animal Feedingstuffs, National Veterinary Research Institute, Al. Partyzantów 57, 24-100 Puławy, Poland
autor
  • Department of Hygiene of Animal Feedingstuffs, National Veterinary Research Institute, Al. Partyzantów 57, 24-100 Puławy, Poland
  • Department of Animal Nutrition and Feed Sciences, National Research Institute of Animal Production, 32-083 Balice n. Kraków, Poland
  • Department of Animal Nutrition and Feed Sciences, National Research Institute of Animal Production, 32-083 Balice n. Kraków, Poland
autor
  • Department of Animal Nutrition and Feed Sciences, National Research Institute of Animal Production, 32-083 Balice n. Kraków, Poland
  • Department of Animal Nutrition and Feed Sciences, National Research Institute of Animal Production, 32-083 Balice n. Kraków, Poland
  • Department of Animal Nutrition and Feed Sciences, National Research Institute of Animal Production, 32-083 Balice n. Kraków, Poland
  • Department of Hygiene of Animal Feedingstuffs, National Veterinary Research Institute, Al. Partyzantów 57, 24-100 Puławy, Poland

Bibliografia

  • Aulrich K, Böhme H, Daenicke R, Halle I, Flachowsky G (2002) Novel feeds – a review of experiments at our Institute. Food Res Int 25: 285-293.
  • Aulrich K, Böhme H, Daenicke R, Halle IT, Flachowsky G (2001) Genetically modified feeds in animal nutrition. 1st Communications: Bacillus thuringiensis (Bt) corn in poultry, pig and ruminant nutrition. Arch Anim Nutr 54: 183-195.
  • Aumaitre A, Aulrich K, Chesson A, Flachowsky G, Piva G (2002) New feeds from genetically modified plants: substantial equivalence, nutritional equivalence, digestibility, and safety for animals and the food chain. Livest Prod Sci 74: 223-238.
  • Beever DE, Kemp CF (2000) Safety issues associated with the DNA in animal feed derived from genetically modified crops. A review of scientific and regulatory procedures. Nutr Abstr Rev 70: 175-182.
  • Beever DE, Phipps RH (2001) The fate of plant DNA and novel proteins in feeds for farm livestock: A United Kingdom perspective. J Anim Sci 79: 290-295.
  • Chambers PA, Duggan PS, Heritage J, Forbes JM (2002) The fate of antibiotic resistance marker genes in transgenic plant feed material fed to chickens. J Antimicrob Chemother 49: 161-164.
  • De Vries J, Meier P, Wackernagel W (2001) The natural transformation of the soil bacteria Pseudomonas stutzeri and Acinetobacter sp. by transgenic plant DNA strictly depends on homologous sequences in the recipient cells. FEMS Microbiol Lett 195: 211-215.
  • Deaville ER, Maddison BC (2005) Detection of transgenic and endogenous plant DNA fragments, in the blood, tissues, and digesta of broilers. J Agric Food Chem 53: 10268-10275.
  • Flachowsky G, Aulrich K, Bohme H, Halle I (2007) Studies on feeds from genetically modified plants (GMP) – Contributions to nutritional and safety assessment. Anim Feed Sci Tech 133: 2-30.
  • Flachowsky G, Halle I, Aulrich K (2005) Long term feeding of Bt-corn – a ten-generation study with quails. Arch Anim Nutr 59: 449-451.
  • Flachowsky G, Chesson A, Aulrich K (2005a) Animal nutrition with feeds from genetically modified plants. Arch Anim Nutr 59: 1-40.
  • Gaines AM, Allee GL, Ratliff BW (2001) Swine digestible energy evaluation of Bt (MON810) and Roundup Ready(R) corn compared with commercial varieties. J Anim Sci 79 (Suppl 1): 109 (Abstr 453).
  • James C (2012) Global Status of Commercialized Biotech/GM Crops: 2012. ISAAA Brief No. 44. ISAAA: Ithaca, NY.
  • Jennings JC, Kolwyck DC, Kays SB, Whetsell AJ, Surber JB, Cromwell GL, Lirette RP, Glenn KC (2003) Determining whether transgenic and endogenous plant DNA and transgenic protein are detectable in muscle from swine fed Roundup Ready soybean meal. J Anim Sci 81: 1447-1455.
  • Klotz A, Mayer J, Einspanier R (2002) Degradation and possible carry over of feed DNA monitored in pigs and poultry. Eur Food Res Technol 214: 271-275.
  • Mazza R, Soave M, Morlacchini M, Piva G, Marocco A (2005) Assessing the transfer of genetically modified DNA from feed to animal tissues. Transgenic Res 14: 775-784.
  • McCann MC, Liu K, Trujillo WA, Dobert RC (2005) Glyphosate-tolerant soybeans remain compositionally equivalent to conventional soybeans (Glycine max L.) during three years of field testing. J Agric Food Chem 53: 5331-5335.
  • Padgette SR, Biest Taylor N, Nida DL, Bailey MR, MacDonald J, Holden LR, Fusch RL (1996) The composition of glyphosate-tolerant soybean seeds is equivalent to that of conventional soybeans. J Nutr 126: 702-716.
  • Reuter T, Aulrich K (2003) Investigations on genetically modified maize (Bt-maize) in pig nutrition: fate of feed-ingested foreign DNA in pig bodies. Eur Food Res Technol 216: 185-192.
  • Rossi F, Morlacchini M, Fusconi G, Pietri A, Mazza R, Piva G (2005) Effect of Bt corn on broiler growth performance and fate of feed-derived DNA in the digestive tract. Poult Sci 84: 1022-1030.
  • Sanden M, Bruce IJ, Rahman MA, Hemre GI (2004) The fate of transgenic sequences present in genetically modified plant products in fish feed, investigating the survival of GM soybean DNA fragments during feeding trials in Atlantic salmon, Salmo salar L. Aquaculture 237: 391-405.
  • Schluter K, Futterer J, Potrykus I (1995) “Horizontal” gene-transfer from a transgenic potato line to a bacterial pathogen (Erwinia chrysanthemi) occurs – if at all – at an extremely low frequency. Biotechnology 13: 1094-1098.
  • Seralini GE, Clair E, Mesnage R, Gress S, Defarge N, Malatesta M, Hennequin D, de Vendomois JS (2012) Long term toxicity of a Roundup herbicide and a Roundup-tolerant genetically modified maize. Food Chem Toxicol 50: 4221-4231.
  • Sissener NH, Sanden M, Bakke AM, Krogdahl A, Hemre GI (2009) A long term trial with Atlantic salmon (Salmo salar L.) fed genetically modified soy; focusing general health and performance before, during and after the parr-smolt transformation Aquaculture 294: 108-117.

Typ dokumentu

Bibliografia

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