Effects of sodium taurocholate and sodium dehydrocholate on bile flow, lipid and bilirubin secretion in sheep

• Autorzy: Bobowiec R. , Studzinski T. , Sikorska M.
• Języki publikacji: EN

Abstrakty

EN

Bobowiec R., Studziński T. and Sikorska M.: Effects of sodium taurocholate and sodium deyhdrocholate on bile flow and lipid and bilirubin secretion in sheep. Acta Physiol. Pol. The investigation was performed on 8 sheep with implanted catheters in the common bile duct and in the cystic duct. Sodium taurocholate and sodium dehydrocholate were infused into the jugular vein at the rate of 50 µmol/min for 20 min. Directly after the termination of the sodium taurocholate infusion, the volume of the secreted bile increased from 8.4-9 µl· kg⁻¹ · min⁻¹ to the highest mean value of 17.8 µl·kg⁻¹ , min⁻¹ , with a simultaneous increase in the concentration of cholates from 1.71 mmol/1 to 4.82 mmol/1 and bilirubin from 271.1 µmol/1 to 461.7 µmol/1. The concentration of cholesterol and phospholipids in the bile also increased, but did not reach statistically significant values. The infusion of sodium dehydrocholate caused an increase in the bile secretion to the highest mean value of 20.59 µ1 · kg⁻¹ · min⁻¹ with a simultaneous decrease in the concentration of bilirubin to 148.75 µmol/1, cholesterol to 233.0 µg/ml, phospholipids to 56.11 µg/ml and cholate to 1.0 mmol/1. The results show that biliary secretion of phospholipids, cholesterol and bilirubin is dependend on the secretion of sodium taurocholate rather than on dehydrocholic acid.

Słowa kluczowe

EN

sodium taurocholate; sodium dehydrocholate; phospholipid; cholesterol; bile flow; lipid; bilirubin; secretion; sheep

• Wydawca: -
• Czasopismo: Acta Physiologica Polonica
• Rocznik: 1990
• Tom: 41
• Numer: 7
• Opis fizyczny: p.146-154,fig.,ref.

Twórcy

autor

Bobowiec R.

• Department of Animal Physiology, Institute of Physiological Sciences, Faculty of Veterinary, Agricultural Academy, Akademicka 12, 20-033 Lublin, Poland

autor

Studzinski T.

• Department of Animal Physiology, Institute of Physiological Sciences, Faculty of Veterinary, Agricultural Academy, Akademicka 12, 20-033 Lublin, Poland

autor

Sikorska M.

• Department of Animal Physiology, Institute of Physiological Sciences, Faculty of Veterinary, Agricultural Academy, Akademicka 12, 20-033 Lublin, Poland

Bibliografia

• 1. Angelico M., Alvaro D., Attili A. F., Cantafora A. (1985): Mechanisms of secretion of biliary phosphatidylocholines: the role of bile acids. Ital. J. Gastroenterol., 17, 278-281.
• 2. Bartlett G. R. (1959): Phosphorus assay in column chromatography. J. Biol. Chem., 234, 466-468.
• 3. Bloomer J. R., Boyer J. L., Klatskin G. (1973): Inhibition of bilirubin excretion in man during dehydrocholate choleresis. Gastroenterol., 65, 929-935.
• 4. Bobowiec R. (1984): Wpływ dożylnych infuzji soli sodowych kwasów żółciowych na wydzielanie żółci i kwasów żółciowych u owiec. Ann. UMCS, Sectio DD, 39, 4, 29-42.
• 5. Engelking L. R., Gronwall R., Anwer M. S. (1980): Effect of dehydrocholic, chenodeoxycholic and taurocholic acids on the excretion of bilirubin. Am. J. Vet. Res., 41, 355-361.
• 6. Entenman C., Holloway R. J., Albright M. L., Leong G. F. (1968): Bile acids and lipid metabolism. Stimulation of bile lipid excretion by various bile acids. Proc. Soc. Exp. Biol. Med., 127, 1003-1006.
• 7. Erlinger S., Dumont M., Berthelot P., Dhumeaux D. (1971): Comparison of the choleretic effects of dehydrocholate and glycodeoxycholate in the rabbit. Digestion, 4, 144-145.
• 8. Esteller A., Gonzales J., Hidalgo F., Lopez M. A. (1984): A comparison of the effect of bile salts on maximal hepatic excretion of certain organic anions in the rabbit under urethane and pentobarbitone. Q. J. Exp. Physiol., 69, 227-234.
• 9. Garcia-Martin J. J., Gonzales J., Esteller A. (1986): Influence of dehydrocholate on bilirubin transport into bile in the rat. Digestion, 33, 80-88.
• 10. Gregory D. H., Vlahcevic Z. R., Schatzki P., Swell L. (1975): Mechanism of secretion of biliary lipids. J. Clin. Invest., 55, 105-114.
• 11. Heath T. (1970): Effect of secretin on bile formation in shep. Q. J. Exp. Physiol., 55, 301-312.
• 12. Leat W. M. F., Harrison F. A. (1969): Lipid digestion in the sheep: effect of bile and pancreatic juice on the lipids of intestinal contents. Q. J. Exp. Physiol., 54, 187-201.
• 13. Levin S. J., Johnston C. G., Boyle A. J. (1961): Spectrophotometric determination of several bile acids as conjugates. Anal. Chem., 33, 1407-1411.
• 14. Lowe P. J., Barnwell S. G., Coleman R. (1984): Rapid kinetic analysis of the bile-salt-dependent secretion of phospholipid, cholesterol and plasma-membrane enzyme into bile. Biochem J., 222, 631-637.
• 15. Malloy H. T., Evelyn K. A. (1937): The determination of bilirubin with the photoelectric colorimeter. J. Biol. Chem., 119, 481-488.
• 16. Parkhill В. J., Bernhardt J. L., Combes B. (1979): Hepatic metabolism of glycodehydrocholic acid in the dog and rat. Proc. Soc. Exp. Biol. Med., 162, 495-502.
• 17. Swell L., Entenman C., Leong G. F., Holloway R. J. (1968): Bile acids and lipid metabolism. IV Influence of bile acids on biliary and liver organelle phospholipids and cholesterol. Am. J. Physiol., 215, 1390-1396.
• 18. Upson D. W., Gronwall R. R., Cornelius С. E. (1970): Maximal hepatic excretion of bilirubin in sheep. Proc. Soc. Exp. Biol. Med., 134, 9-12.
• 19. Zlatkis A., Zak B., Boyle A. J. (1953): A new method for the direct determination of serum cholesterol. J. Lab. Clin. Med., 41, 486-492.