The loop-mediated isothermal amplification assay for rapid diagnosis of Babesia canis canis infections in dogs

• Autorzy: Adaszek L. , Jankowska M. , Kalinowski M. , Banach T. , Wulupek D. , Winiarczyk S.
• Języki publikacji: EN

Abstrakty

EN

The aim of this study was to use a rapid and easy DNA-based test, the loop-mediated isothermal amplification (LAMP), for diagnosis of Babesia canis canis infections in dogs. 10 DNA samples of 18S RNA-A and 10 DNA samples of 18S RNA-B of B. canis canis were used in the study. LAMP method could successfully detect DNA in all examined samples down to 0.1 pg dilution. Obtained results suggest that this method has high specificity and sensitivity and can be applied in analytical laboratories in diagnosis of canine babesiosis.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2013
• Tom: 16
• Numer: 1
• Opis fizyczny: p.131-133,fig.,ref.

Twórcy

autor

Adaszek L.

• Department of Epizootiology and Infectious Diseases, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Gleboka 30, 20-612 Lublin, Poland

autor

Jankowska M.

• Department of Biotechnology and Food Microbiology, Faculty of Food Science and Nutrition, University of Life Sciences in Poznan, Wojska Polskiego 48, 60-627 Poznan, Poland

autor

Kalinowski M.

• Department of Epizootiology and Infectious Diseases, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Gleboka 30, 20-612 Lublin, Poland

autor

Banach T.

• Department of Epizootiology and Infectious Diseases, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Gleboka 30, 20-612 Lublin, Poland

autor

Wulupek D.

• Department and Clinic of Animal Internal Diseases, University of Life Sciences in Lublin, Gleboka 30, 20-612 Lublin, Poland

autor

Winiarczyk S.

• Department of Epizootiology and Infectious Diseases, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Gleboka 30, 20-612 Lublin, Poland

Bibliografia

• Adaszek Ł, Winiarczyk S, Skrzypczak M (2009) The clinical course of babesiosis in 76 dogs infected with protozoan parasites Babesia canis canis. Pol J Vet Sci 12: 81-87.
• Iseki H, Alhassan A, Ohta N, Thekisoe OM, Yokoyama N, Inoue N, Nambota A, Yasuda J, Igarashi I (2007) Development of a multiplex loop-mediated isothermal amplification (mLAMP) method for the simultaneous detection of bovine Babesia parasites. J Microbiol Methods71: 281-287.
• ISO/TR13843 (2000) Water quality-Guidance on validation of microbiological methods. International Organization for Standardisation, Geneva, Switzerland.
• Mosqueda J, Olvera-Ramirez A, Aguilar-Tipacamu G, Canto GJ (2012) Current advances in detection and treatment of babesiosis. Curr Med Chem 19: 1504-1518.
• Muller H, Aysul N, Liu Z, Salih DA, Karagenc T, Beyer D, Kullmann B, Ahmed JS, Seitzer U (2010) Development of a loop-mediated isothermal amplification (LAMP) assay for rapid diagnosis of Babesia canis infections. Transbound Emerg Dis 57: 63-65.

Uwagi

PL

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