Phenotypical identyfication of atypical Staphylococcus aureus strains isolated from milk of cows from one herd

• Autorzy: Malinowski E. , Lassa H. , Tomaszewska J. , Malkinska-Horodyska M.
• Języki publikacji: EN

Abstrakty

EN

The aim of the study was to characterise the free coagulase-negative Staphylococcus sp. strains isolated from normal (9.4%) and inflamed quarter milk samples of dairy cows in one herd localised in southern Poland. The isolates were examined phenotypically by cultural features, tube coagulase test, clumping factor (CF), and commercial agglutination test. Total number of 427 strains were cultivated on different media: Congo red agar, DNase medium, mannitol salt agar, and crystal violet agar. About 25% of the strains were examined by the disc diffusion method for their sensitivity to chosen antibiotics. All of the analysed strains showed α, ß, α+ß, or lh type of haemolysis on plate with 5% sheep blood agar, and had positive reaction in CF and agglutination tests, but were negative in tube coagulase test. These strains showed DNase activity and ability to mannitol fermentation; however, none of the strains produced slime. There were variations in growth on crystal violet agar: examined strains belonged to three types of growth (A, E, and C). The strains were highly susceptible in vitro to oxacillin (99.2%), cefoperazone (95.2%), and amoxicillin with clavulanic acid (93.6%), and highly resistant to neomycin (27.2%), tetracycline (21.6%), and streptomycin (20.8%). In conclusion, it can be stated that intramammary infections in cows can be caused by atypical free coagulase-negative strains of Staphylococcus aureus.

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2011
• Tom: 55
• Numer: 2
• Opis fizyczny: p.211-215,fig.,ref.

Twórcy

autor

Malinowski E.

• Department of Pathophysiology of Reproduction and Mammary Gland, National Veterinary Research Institute, 85-090 Bydgoszcz, Poland

autor

Lassa H.

autor

Tomaszewska J.

autor

Malkinska-Horodyska M.

Bibliografia

• 1. Amorena B., Gracia E., Monzon M., Leiva J., Oteiza C., Perez M., Alabart J.M., Hernandez-Yago J.: Antibiotic susceptibility assay for Staphylococcus aureus in biofilms developer in vitro. J Antimicrob Chemother 1999, 44, 43- 55.
• 2. Bannerman T.L.: Staphylococcus, Micrococcus, and other catalase-positive cocci that grow aerobically. Manual of clinical microbiology, ASM Press, Washington DC, 2003.
• 3. Bar D., Tauer L.W., Bennett G., Gonzalez R.W., Hertl J.A., Schukken Y.H., Schulte H.F., Welcome F.L., Gröhn Y.T.: The cost of generic clinical mastitis in dairy cows as estimated by using dynamic programming. J Dairy Sci 2008, 91, 2205-2214.
• 4. Barkema H.W., Schukken Y.H., Zadoks R.N.: Invited review. The role of cow, pathogen, and treatment regimen in the therapeutic success of bovine Staphylococcus aureus mastitis. J Dairy Sci 2006, 89, 1877-1895.
• 5. Blake J.E., Metcalfe M.A.: A shared noncapsular antigen is responsible for false-positive reactions by Staphylococcus epidermidis in commercial agglutination tests for Staphylococcus aureus. J Clin Microbiol 2001, 39, 544-550.
• 6. Boerlin P., Kuhnert P., Hüssy D., Schaellibaum M.: Methods for identification of Staphylococcus aureus isolates in cases of bovine mastitis. J Clin Microbiol 2003, 41, 767-771.
• 7. Cabral da Silva Santos O., Mdenezes Barros E., Vasconcelos Paida Brito M.A., do Carmo de Freire Bastos M., Netto dos Santos K.R., Giambiagi-deMarval M.: Identification of coagulase-negative staphylococci from bovine mastitis using RFLP-PCR of the groEL gene. Vet Microbiol 2008, 130, 134-140.
• 8. Ciftci A., Findik A., Onuk E.E., Savasan S.: Detection of methicillin resistant and slime factor production of Staphylococcus aureus in bovine mastitis. Braz J Microbiol 2009, 40, 254-261.
• 9. Davenport D.S., Massanari R.M., Pfaller M.A., Baller M.J., Streed S.A., Hierholzer W.J.: Usefulness of a test for slime production as a marker of clinically significant infections with coagulase-negative staphylococci. J Infect Dis 1986, 153, 332-339.
• 10. Fijałkowski K., Czernomysy-Furowicz D., Ferlas M.: Staphylococcus aureus versus the immune system. Post Mikrobiol 2008, 47, 497-501.
• 11. Fox L.K., Beser T.E., Jackson S.M.: Evaluation of coagulase-negative variant of Staphylococcus aureus as a cause of intramammary infections in a herd of dairy cattle. J Am Vet Med Assoc 1996, 209, 1143-1146.
• 12. Freeman R., Hudson S.J., Burdess D.: Crystal violet reactions of fresh clinical isolates of Staphylococcus aureus from two British hospitals. Epidemiol Infect 1990, 105, 493-500.
• 13. Garbacz K., Piechowicz L., Wiśniewska K., Galiński J.: Characteristics of coagulase-negative and CF-negative methicillin-resistant Staphylococcus aureus strains. Med Dośw Mikrobiol 2002, 54, 1-8.
• 14. Garbacz K., Goliński J.: Atypical Staphylococcus aureus strains defective in coagulase or clumping factor (CF). Post Microbiol 2006, 45, 39-43.
• 15. Gündoğan N., Citak S., Turan E.: Slime production, DNase activity and antibiotic resistance of Staphylococcus aureus isolated from raw milk, pasteurized milk and ice cream samples. Food Control 2006, 17, 389-392.
• 16. Hall S.L.: Coagulase negative staphylococcal infections in neonates. Pediatric Infect Dis J 1991, 10, 57-76.
• 17. Huijps K., Lam T.J.G.M., Hogeeven H.: Costs of mastitis: facts and perception. J Dairy Res 2008, 75, 113-120.
• 18. Ishii Y., Alba J., Maehara C., Murakami H., Matsumoto T., Tateda K., Furuya N., Iwata M., Yamaguchi K.: Identification of biochemically atypical Staphylococcus aureus clinical isolates with Tyree automatem identification systems. J Med Microbiol 2006, 55, 387- 392.
• 19. Laevens H., Devriese L.A., Deluyker H., Hommez J., de Kruif A.: An atypical Staphylococcus aureus intramammary infection in a dairy herd. Vet Microbiol 1996, 52, 271-275.
• 20. Lee J.H.: Methicillin (oxacillin) - resistant Staphylococcus aureus strains isolated from major food animals and their potential transmission to humans. Appl Environ Microbiol 2003, 69, 6489-6494.
• 21. Leitner G., Lubashevsky E., Glickman A., Winkler M., Saran A., Trainin Z.: Development of a Staphylococcus aureus vaccine against mastitis in dairy cows. I. Challenge trails. Vet Immunol Immuno-Pathol 2003, 93, 31-38.
• 22. Malinowski E., Kłossowska A.: Diagnostyka zakażeń i zapaleń gruczołu mlekowego krów. Published by National Veterinary Research Institute, Pulawy, Poland, 2002.
• 23. Malinowski E., Lassa H., Kłossowska A., Smulski S., Markiewicz H., Kaczmarowski M.: Etiological agents of dairy cows' mastitis in western part of Poland. Pol J Vet Sci 2006, 9, 191-194.
• 24. Malinowski E., Lassa H., Kłossowska A., Smulski S., Kaczmarowski M.: Atypical Staphylococcus aureus as an aetiological agent of mastitis in cows. Bull Vet Inst Pulawy 2009, 53, 383-387.
• 25. Mastunga J., Kamata S., Kakuchi N., Uchida K: Characteristics of Staphylococus aureus peracute, acute and chronic bovine mastitis. J Vet Med Sci 1993, 55, 297- 300.
• 26. Młynarczyk A., Młynarczyk G., Szymanowska A.: Use of PCR for evaluating of coa and nuc genes in methicillin- resistant, coagulase-negative strains of Staphylococcus aureus (MRSA-CN). Med Dośw Mikrobiol 2000, 52, 217-222.
• 27. Murray P.R., Baron E.K., Jorgensen J.H., Pfaller M.A., Yolken R.H.: Manual of Clinical Microbiology, American Society for Microbiology, Washington DC, 2003.
• 28. Naidu A.S.: A study on enterotoxins produced by foodborne S.aureus. Thesis, Osmania University, Hyderabad, India, 1984.
• 29. Park K.Y., Koo H.C., Kim S.H., Hwang S.H., Jung W.K., Kim W.K., Shin S., Kim R.T., Park Y.H.: The analysis of milk components and pathogenic bacteria isolated from bovine raw milk in Korea. J Dairy Sci 2007, 90, 5405- 5414.
• 30. Pfaller M.A., Herwaldt L.A.: Laboratory, clinical and epidemiological aspects of coagulase negative staphylococci. Clin Microbiol 1988, 37, 201-205.
• 31. Pyörälä S., Taponen S.: Coagulase-negative staphylococci - emerging mastitis pathogens. Vet Microbiol 2009, 134, 3-8.
• 32. Sachanowicz J., Jakubczak A., Piechota M.: Phenotype and genotype traits of S. aureus strains isolated form mastitis milk samples. Medycyna Wet 2005, 61, 1370- 1373.
• 33. Schukken Y.H., Gonzalez R.N., Tikofsky L.L., Schulte H.F., Santisteban C.G., Welcome F.L., Bannett G.J., Zurakowski M.J., Zadoks R.N.: CNS mastitis: nothing to worry about ? Vet Microbiol 2009, 134, 9-14.
• 34. Schwarzkopf A., Schmidt-Rotte H., Schmidt H.: Molecular analysis of nosocomial infection by oxacillin- resistant Staphylococcus aureus lacking protein A and clumping factor. Lancet 1992, 340, 621.
• 35. Sol J., Sampimon O.C., Barkema H.W., Schukken Y.H.: Factors associated with cure after therapy of clinical mastitis caused by Staphylococcus aureus. J Dairy Sci 2000, 83, 278-284.
• 36. Takeshige K., Watanabe K., Igarashi H., Shingaki M., Terayama T.: Detection of Staphylococcus aureus in bovine mastitis and some characteristics with special reference to enterotoxin producibility and coagulase types of isolates. Jpn J Vet Sci 1983, 45, 355-362.
• 37. Türkyilmaz S., Eskiizmirliler S.: Detection of slime factor production and antibiotic resistance in Staphylococcus strains isolated from various animal clinical samples. Turk J Vet Anim Sci 2006, 30, 201-206.
• 38. Vandenesch F., Bes M., Lebeau C., Greenland T., Brun Y., Etienňe J.: Coagulase-negative Staphylococcus aureus. Lancet 1993, 342, 995-996.
• 39. Watts J.R., Salmon J.A.: Activity of selected antimicrobial agents against strains of Staphylococcus aureus isolated from bovine intramammary infections that produce ß-lactamase. J Dairy Sci 1997, 80, 788-791.
• 40. Wiśniewska K., Garbacz K., Piechowicz L.: Genotypie screening of atypical Staphylococcus aureus strains isolated from clinical samples for presence of selected adhesin genes. Med Mal Infect 2008, 38, 549-553.
• 41. Woo P.C.Y., Leung A.S.P., Leung K.W., Yuen K.Y.: Identification of slide coagulase positive, tube coagulase negative Staphylococcus aureus by 16S ribosomal RNA gene sequencing. J Clin Pathol Mol Pathol 2001, 54, 244- 247.