Changes in expression of proteins involved in alleviation of Fe-deficiency by sulfur nutrition in Brassica napus L.

• Autorzy: Muneer S. , Lee B. R. , Bae D. W. , Kim T. H.
• Języki publikacji: EN

Abstrakty

EN

In order to characterize the significance of sulfur (S) nutrition in protein expression under iron (Fe)-deficient conditions, gel-based proteomic analysis was performed with the leaves of Brassica napus exposed to S and Fe combined treatments: sufficient in S and Fe (+S/+Fe, control), sufficient S but Fe deprived (+S/−Fe), deprived S but sufficient Fe (−S/+Fe), and deprived S and Fe (−S/−Fe). The resulting data showed that 15 proteins were down-regulated due to production of oxidative damage as indicated by H2O2 and O 2 −1 localizations and due to leaf chlorosis in leaves in S-deprived leaves either in presence (−S/+Fe) or absence of Fe (−S/−Fe), whereas these down-regulated proteins were well expressed in the presence of S (+S/−Fe) compared to control (+S/+Fe). In addition, two proteins were up-regulated under S-deprived condition in presence (−S/+Fe) and absence of (−S/−Fe) Fe. The functional classification of these identified proteins was estimated that 40 % of the proteins belong to chloroplast precursor, and rest of the proteins belongs to hypothetical proteins, RNA binding, secondary metabolism and unknown proteins. On the other hand, five protein spots from S deprived (−S/+Fe) and ten spots from Fe deprived (−S/−Fe) conditions were absent, whereas they were well expressed in presence of S (+S/−Fe) compared to control plants (+S/+Fe). These results suggest that sulfur nutrition plays an important role in alleviating protein damage in Fe-deficient plants and adaptation to Fe-deficiency in oilseed rape.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2013
• Tom: 35
• Numer: 10
• Opis fizyczny: p.3037-3045,fig.,ref.

Twórcy

autor

Muneer S.

• Department of Animal Science, Institute of Agricultural Science and Technology, College of Agriculture and Life Science, Chonnam National University, Buk-Gwangju, P.O. Box 205, Gwangju 500-600, Korea

autor

Lee B. R.

• Environmental Friendly Agricultural Bio-material Research Team, College of Agriculture, Chonnam National University, Gwangju 500-757, Korea

autor

Bae D. W.

• Central Instrument Facility, Biomaterial Analytical Lab, Gyeongsang National University, Jinju 660-701, Korea

autor

Kim T. H.

• Department of Animal Science, Institute of Agricultural Science and Technology, College of Agriculture and Life Science, Chonnam National University, Buk-Gwangju, P.O. Box 205, Gwangju 500-600, Korea

Bibliografia

• Abdallah M, Dubousset L, Meuriot F, Etienne P, Avice JC, Ourry A (2010) Effect of mineral sulphur availability on nitrogen and sulphur uptake and remobilization during the vegetative growth of Brassica napus L. J Exp Bot 61(10):2635–2646
• Astolfi S, Zuchi S, Hubberten HM, Pinton R, Hoefgen R (2012) Supply of sulphur to S-deficient young barley seedlings restores their capability to cope with iron shortage. J Exp Bot 61(3):799–806
• Balk J, Pilon M (2011) Ancient and essential: the assembly of ironsulfur cluster in plants. Trends Plant Sci 16(4):218–226
• Beven M, Bancroft I et al (1998) Analysis of 1.9 Mb contiguous sequence from chromosome 4 of Arabidopsis thaliana. Nature 391:485–493
• Blake-Kalff MMA, Hawkesford MJ, Zhao FJ, McGrath SP (2000) Diagnosing sulfur deficiency in field-grown oilseed rape (Brassica napus L.) and wheat (Triticum aestivum L.). Plant Soil 225:95–107
• Briat JF, Curie C, Gaymard F (2007) Iron utilization and metabolism in plants. Curr Opin Plant Biol 10:276–282
• Briat JF, Duc C, Ravet K, Gaymard F (2010) Ferritin and iron storage in plants. Biochim Biophys Acta 8:806–814
• Choudhary MK, Basu D, Datta A, Chakraborty N, Chakroborty S (2009) Dehydration-responsive nuclear proteome of rice (Oryza sativa L.) illustrates protein network, novel regulators of cellular adaptation, and evolutionary. Mol Cell Proteomics 8(7): 1579–1598
• Douchkov D, Herbik A, Koch G, Mock HP, Melzer M, Stephan UW, Bäumlein H (2002) Nicotianamine synthase: gene isolation, gene transfer and application for the manipulation of plant iron assimilation. Plant Soil 241:115–119
• Droux M (2004) Sulfur assimilation and role of sulfur in plant metabolism: a survey. Photosynth Res 79(3):331–348
• Feng J, Barker AV (1993) Polyamine concentration and ethylene evolution in tomato plants under nutritional stress. Hort Sci 28:109–110
• Guikema JA, Sherman LA (1983) Chlorophyll-protein organization of membranes from the Cyanobacterium Anacystisnidulans. Arch Biochem Biophys 220:155–166
• Hesse H, Hoefgen R (2003) Molecular aspects of methionine biosynthesis in Arabidopsis and potato. Trends Plant Sci 8: 259–262
• Imsande J (1999) Iron-sulfur clusters: formation, perturbation, and physiological functions. Plant Physiol Biochem 37:87–97
• Kim TH, Lee BR, Jung WJ, Kim KY, Avice JC, Ourry A (2004) De novo protein synthesis in relation to ammonia and proline accumulation in water stressed white clover. Funct Plant Biol 31:847–855
• Landry LG, Pell EJ (1993) Modification of RuBisCO and altered proteolytic activity in O3-stressed hybrid poplar (populus maximowizzi 9 trichorcarpa). Plant Physiol 101:1355–1362
• Lee BR, Muneer S, Kim KY, Avice JC, Ourry A, Kim TH (2013) S-deficiency responsive accumulation of amino acids is mainly due to hydrolysis of the previously synthesized proteins—not to de novo synthesis in Brassica napus. Physiol Plant 147:369–380
• Leustek T, Saito K (1999) Sulfate transport and assimilation in plants. Plant Physiol 120:637–643
• Lindsay WL, Schwab AP (1982) The chemistry of iron in soils and its availability to plants. J Plant Nutr 5:821–840
• Luo S, Ishida H, Makino A, Mae T (2002) Fe2+-catalyzed site specific cleavage of the large subunit of ribulose 1,5-bisphosphatecarboxylase close to the active site. J Biol Chem 277: 12382–12387
• Marschner H, Romheld V, Kissel M (1986) Different strategies in higher plants in mobilization and uptake of iron. J Plant Nutr 9:695–713
• McGrath SP, Zhao FJ (1996) Sulphur uptake, yield response and the interactions between N and S in winter oilseed rape (Brassica napus L.). J Agric Sci 126:53–62
• Morales F, Grasa R, Abadia A, Abadia J (1998) Iron chlorosis paradox in fruit trees. J Plant Nutri 21:815–825
• Muneer S, Kim TH, Qureshi MI (2012) Fe modulates Cd-induced oxidative stress and the expression of stress responsive proteins in the nodules of Vigna radiata. Plant grow reg 68:421–433
• Nikiforova VJ, Kopka J, Tolstikov V, Fiehn O, Hopkins L, Hawkesford MJ, Hesse H, Hoefgen R (2005) Systems rebalancing of metabolism in response to sulphur deprivation, as revealed by metabolome analysis of Arabidopsis plants. Plant Physiol 138:304–318
• Pandey A, Choudhary MK, Bhushan D, Chattopadhyay A, Chakraborty S, Datta A, Chakroborty N (2006) The nuclear proteome of chick pea (Cicer arietinum L.) reveals predicted and unexpected proteins. J Proteome Res 5(12):3301–3311
• Qureshi MI, Muneer S, Bashir H, Ahmad J, Iqbal M (2010) Nodule physiology and proteomics of stressed legumes. Adv Bot Res 56:1–38
• Romera FJ, Alcántara E (2004) Ethylene involvement in the regulation of Fe-deficiency stress responses by strategy I plants. Func Plant Biol 31:315–328
• Shetty AS, Miller GW (1966) Influence of iron chlorosis on pigment and protein metabolism in leaves of Nicotiana tabacum L. Plant Physiol 41:415421
• Spiller S, Terry N (1980) Limiting factors in photosynthesis II. Iron stress diminishes photochemical capacity by reducing the number of photosynthetic units. Plant Physiol 65:121–125
• Szacilowski K, Chmura A, Stasicka Z (2005) Interplay between iron complexes, nitric oxide and sulfur ligands: structure, (photo) reactivity and biological importance. Coord Chem Rev 249:2408–2436
• Timperio AM, D’Amici GM, Barta C, Loreto F, Zolla L (2007) Proteomics, pigment composition, and organization of thylakoid membranes in iron deficient Spinach leaves. J Exp Bot 58(13):3695–3710
• Zuchi S, Cesso S, Varanini Z, Pinton R, Astolfi S (2009) Sulphur deprivation limits Fe-deficiency response in tomato plants. Planta 230(1):85–94
• Zuchi S, Cesso S, Astolfi S (2012) High S supply improves Fe accumulation in durum wheat plants grown under Fe limitation. Environ Exp Bot 77:25–32

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