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2015 | 18 | 3 |
Tytuł artykułu

Differentially expressed proteins in the blood serum of piglets in response to a diet supplemented with inulin

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EN
Abstrakty
EN
In the present study we introduced a two-dimensional electrophoresis and matrix-assisted laser desorption/ionisation time of flight mass spectrometry-based proteomic workflow to identify proteins that show altered expression as a result of the addition of 2% of water extract of inulin-type fructans to the diet of growing piglets. This analysis allowed us to detect an average of 240 spots per gel with a mass range from 10 to 250 kDa and a pH ranging from 3 to 10. Twenty protein spots were found to show statistically significant differences in their expression. Of these, 7 protein spots were up-regulated, whereas 13 showed down-regulation in response to the experimental diet. In total, 13 spots were identified, representing 8 distinct gene products. The experimental diet caused a significant change in proteins directly or indirectly involved in hemostasis and the innate immune response. Increased levels of fibrinogen along with decreased plasminogen expression may indicate that a fructan-rich diet favours the deposits of fibrin and promotes blood clotting. We also found increased expression of vitronectin and the alpha subunit of the complement component C8 which may protect the host organism against excessive cytolitic activity of the activated complement. The piglets from the experimental group had slightly increased values of IgG and IgA, whereas the IgM level tended to be decreased. The fructan-rich diet did not have any influence on plasma total cholesterol, HDL and LDL cholesterol and triglyceride levels.
Słowa kluczowe
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-
Rocznik
Tom
18
Numer
3
Opis fizyczny
p.541-548,fig.,ref.
Twórcy
  • Department of Physiology, Cytobiology and Proteomics, Faculty of Biotechnology and Animal Husbandry, West Pomeranian University of Technology, Doktora Judyma 6, 71-466 Szczecin, Poland
  • Department of Physiology, Cytobiology and Proteomics, Faculty of Biotechnology and Animal Husbandry, West Pomeranian University of Technology, Doktora Judyma 6, 71-466 Szczecin, Poland
autor
  • Department of Physiology, Cytobiology and Proteomics, Faculty of Biotechnology and Animal Husbandry, West Pomeranian University of Technology, Doktora Judyma 6, 71-466 Szczecin, Poland
autor
  • Department of Monogastric Nutrition, Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, Instytucka 3, 05-110, Jabłonna, Poland
  • Department of Physiology, Cytobiology and Proteomics, Faculty of Biotechnology and Animal Husbandry, West Pomeranian University of Technology, Doktora Judyma 6, 71-466 Szczecin, Poland
autor
  • Department of Monogastric Nutrition, Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, Instytucka 3, 05-110, Jabłonna, Poland
autor
  • Department of Monogastric Nutrition, Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, Instytucka 3, 05-110, Jabłonna, Poland
autor
  • Department of Monogastric Nutrition, Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, Instytucka 3, 05-110, Jabłonna, Poland
Bibliografia
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  • Sharp AM, Stein PE, Pannu NS, Carrell RW, Berkenpas MB, Ginsburg D, Lawrence DA, Read RJ (1999) The active conformation of plasminogen activator inhibitor 1, a target for drugs to control fibrinolysis and cell adhesion. Structure 7: 111-118.
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  • Su HR (1996) S-protein/vitronectin interaction with the C5b and the C8 of the complement membrane attack complex. Int Arch Allergy Immunol 110: 314-317.
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  • Xu D, Baburaj K, Peterson CB, Xu Y (2001) Model for the three-dimensional structure of vitronectin: predictions for the multi-domain protein from threading and docking. Proteins 44: 312-320.
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Typ dokumentu
Bibliografia
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