PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2014 | 63 | 3 |

Tytuł artykułu

Disc-diffusion and PCR detection of methicillin resistance in environmental airborne strains of Staphylococcus spp.

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
The aim of this study was to assess the species composition of airborne Staphylococcus spp. in public premises, to determine the methicillin resistance of the isolates and the prevalence of mecA gene, determining resistance to β-lactams. In total 65 Staphylococcus strains were isolated from 54 sites. Four strains exhibited phenotypic methicillin resistance, while the presence of mecA gene was found in 11 strains. The results of both assays were compared, showing that the phenotypic tests revealed methicillin resistance only in 36% of the examined samples. This study revealed high species diversity among airborne Staphylococcus spp. population, which consists of multidrug resistant strains.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

63

Numer

3

Opis fizyczny

p.363-368,ref.

Twórcy

  • Department of Microbiology, University of Agriculture in Krakow, Mickiewicza Ave.24/28, 30-059 Krakow, Poland
  • Department of Microbiology, University of Agriculture in Krakow, Mickiewicza Ave.24/28, 30-059 Krakow, Poland
  • Department of Microbiology, University of Agriculture in Krakow, Mickiewicza Ave.24/28, 30-059 Krakow, Poland

Bibliografia

  • American Journal of Infection Control. 1999. National Nosocomial Infections Surveillance (NNIS) System report, data summary from January 1990–May 1999, issued June 1999. Am. J. Infect. Control 27:520–532.
  • Banerjee R., M. Gretes, C. Harlem, L. Basuino and H. Chambers. 2010. A mecA-negative strain of methicillin-resistant Staphylococcus aureus with high-level β-lactam resistance contains mutations in three genes. Antimicrob. Agents Chemother. 54(11): 4900–4902.
  • Bastos M.C., P.J. Mondino, M.L. Azevedo, K.R. Santos and M. Giambiagi-deMarval. 1999. Molecular characterization and transferamong Staphylococcus strains of a plasmid conferring high-level resistance to mupirocin. Eur. J. Clin. Microbiol. Infect. Dis. 18: 393–398.
  • Bonetta S.A., S.I. Bonetta, S. Mosso, S. Sampo and E. Carraro. 2010. Assessment of microbiological indoor air quality in an Italian office building equipped with an HVAC system. Environ. Monit. Assess. 161(1–4): 473–83.
  • Chambers H.F. 1997. Methicillin resistance in staphylococci: molecular and biochemical basis and clinical implications. Clin. Microbiol. Rev. 10(4): 781–91.
  • EUCAST. 2012. European Committee on Antimicrobial Susceptibility Testing.
  • Gaillot O., M. Wetsch, N. Fortineau, P. Berche. 2000. Evaluation of CHROMagar Staph. aureus, a new chromogenic medium, for isolation and presumptive identification of Staphylococcus aureus from human clinical specimens. J. Clin. Microbiol. 38(4): 1587–91.
  • Gąska-Jedruch U. and M.R. Dudzinska. 2009. Microbiological pollution in indoor air, pp. 31–41. In: Ozonek J. and M. Pawłowska (eds). Polish environmental engineering five years after the UE accession. Polish Academy of Sciences, Lublin
  • Geha D.J., J.R. Uhl, C.A. Gustaferro and D.H. Persing. 1994. Multiplex PCR for identification of methicillin-resistant Staphylococci in the clinical laboratory. J. Clin. Microbiol. 32(7): 1768–1772.
  • Georgopapadakou N.H. 1993. Penicillin-binding proteins and bacterial resistance to beta-lactams. Antimicrob. Agents Chemother. 37(10): 2045–2053.
  • Gołofit-Szymczak M. and J. Skowron. 2005. Microbiological hazards in offices (in Polish). Occupational Safety 3: 29–31.
  • Idzik D., R. Wojtyczka, M. Kępa, Z. Wydmuch, J. Pacha, S. Głab,I. Wróblewska and O. Skowronek-Ciołek. 2000. Evaluation of methi-illin-resistance in Staphylococcus aureus by the agar disk diffusion method and PCR (in Polish). Exp. Med. Microbiol. 52(4): 327–332.
  • Kloos W.E. and K.H. Schleifer. 1975. Isolation and characterization of Staphylococci from human skin II. Descriptions of four new species: Staphylococcus warneri, Staphylococcus capitis, Staphylococcus hominis, and Staphylococcus simulans. Int. J. Syst. Bacteriol. 25: 62–79.
  • Kloos W.E. and T.L. Bannerman. 1994. Update on clinical significance of coagulase-negative staphylococci. Clin. Microbiol. Rev. 7: 117–140.
  • Lee J.H. and W.K. Jo. 2006. Characteristics of indoor and outdoor bioaerosols at Korean apartment buildings. Environ. Res. 101: 11–17.
  • Murray P.E. 2007. Manual of Clinical Microbiology. 9th ed. ASM Press, Washington D.C.
  • Patrick C.C. 1990. Coagulase-negative staphylococci: pathogens with increasing clinical significance. J. Pediatr. 116: 497–507.
  • von Eiff C., R.A. Proctor and G. Peters. 2001. Coagulase-negative staphylococci. Pathogens have major role in nosocomial infections. Postgrad. Med. 110: 63–4, 69–70, 73–6.
  • Wanner H., A. Verhoeff, A. Colombi, B. Flannigan, S. Gravesen, A. Mouilleseaux, A. Nevalainen, J. Papadakis and K. Seidel. 1993. European Collaborative Action Indoor air quality and its impact on man, Environment and Quality of Life, Report No. 12 Biological Particles in Indoor Environments. Italy.
  • York M.K., L. Gibbs, F. Chehab and G.F. Brooks. 1996. Comparison of PCR detection of mecA with standard susceptibility testing methods to determine methicillin resistance in coagulase-negative staphylococci. J. Clin. Microbiol. 34(2): 249–53.
  • Zmysłowska I. and B. Jackowska. 2005. The occurrence of the airborne bacteria genus Staphylococcus in the city of Olsztyn (in Polish). Arch. Environ. Prot. 31(1): 13–24.
  • Żabicka D. and W. Hryniewicz. 2010. Recommendations for the selection of the bacterial susceptibility tests to antibiotics and chemotherapeutics 2010. Susceptibility determination of Gram-positive cocci of the genus Staphylococcus spp. (in Polish). National Reference Center for Antibiotic Resistance. Warszawa.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-de757d9f-b525-4bb3-b99b-2bb552455f3d
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.