An efficient method for selecting stable tester strains of vegetative compatibility groups in Verticillium dahliae

• Autorzy: Rataj-Guranowska M.
• Języki publikacji: EN

Abstrakty

EN

The way compatible pairs of nitrate non-utilizing mutants (nit) are selected is usually not explained and remains unclear whether these pairs are representative for an isolate or strain. In addition, tester strains of Verticillium dahliae vegetative compatibility groups (VCGs) cross-react with at least one pair of tester strains of another VCG, and although it is a common knowledge of scientists working with the fungus that reversion of nit mutants to wild type occurs far too often, this fact is rarely mentioned in papers. To overcome the above problems, a protocol was developed for the generation of large number of nit mutants from any given isolate and to ensure that compatible pairs of mutants are indeed stable and the most frequent within the putative tester mutants produced from each isolate. Thus, we provide a reproducible and objective way of selecting V. dahliae tester strains for each isolate and VCG. Although VC grouping is based on the formation of stable heterokaryons, we demonstrate in this work that cross-reactions cannot be eliminated and that strict genetic barriers between two main VCG groups are absent in V. dahliae.

Słowa kluczowe

EN

efficient method; group tester; isolate tester; vegetative compatibility; Verticillium dahliae

• Wydawca: -
• Czasopismo: Journal of Plant Protection Research
• Rocznik: 2016
• Tom: 56
• Numer: 2
• Opis fizyczny: p.163-166,fig.,ref.

Twórcy

autor

Rataj-Guranowska M.

• Collection of Plant Pathogens, Institute of Plant Protection - National Research Institute, Wladysława Wegorka 20, 60-318 Poznan, Poland

Bibliografia

• Correll J.C., Klittich C.J.R., Leslie J.F. 1987. Nitrate nonutilizing mutants of Fusarium oxysporum and their use in vegetative compatibility tests. Phytopathology 77 (12): 1640–1646.
• Dervis S., Erten L., Soylu S., Tok F.M., Kurt S., Yildiz M., Soylu E.M. 2007. Vegetative compatibility groups in Verticillium dahliae isolates from olive in western Turkey. European Journal of Plant Pathology 119 (4): 437–447.
• El-Bebany A.F., Alkher H., Adam L.R., Daayf F. 2013. Vegetative compatibility of Verticillium dahliae isolates from potato and sunflower using nitrate non-utilizing (nit) mutants and PCR-based approaches. Canadian Journal of Plant Pathology 35 (1): 1–9.
• Elena K., Paplomatas E.J. 1998. Vegetative compatibility groups within Verticillium dahliae isolates from different hosts in Greece. Plant Pathology 47 (5): 635–640.
• Hiemstra J.A., Rataj-Guranowska M. 2003. Vegetative compatibility groups in Verticillium dahliae isolates from the Netherland as compared to VCG diversity in Europe and in the USA. European Journal of Plant Pathology 109 (8): 827–839.
• Joaquim T.R., Rowe R.C. 1990. Reassessment of vegetative compatibility relationships among strains of Verticillium dahliae using nitrate – nonutilizing mutants. Phytopathology 80 (11): 1160–1166.
• Papaioannou J.A., Typas M.A. 2015. High-thrughput assessment and genetic investigation of vegetative compatibility in Verticillium dahliae. Journal of Phytopathology 163 (6): 475–485.
• Rataj-Guranowska M. 2006. Vegetative compatibility in Verticillium dahliae from several European countries. Phytopathologia Polonica 42: 5–12.
• Rataj-Guranowska M., Łukaszewska-Skrzypniak N. 2010. Does vegetative compatibility group 1 exist in Verticillium dahliae? Phytopathologia 55: 61–64.
• Strausbaugh C.A. 1993. Assessment of vegetative compatibility and virulence of Verticillium dahliae isolates from Idaho potatoes and tester strains. Phytopathology 83 (1): 1253–1258.
• Zeise K., Tiedemann A. von 2001. Morphological and physiological differentiation among vegetative compatibility groups of Verticillium dahliae in relation to V. longisporum. Journal of Phytopathology 149 (7–8): 469–475.

Identyfikatory

DOI

10.1515/jppr-2016-0028