Asymbiotic seed germination of Cymbidium bicolor Lindl. (Orchidaceae) and the influence of mycorrhizal fungus on seedling development

• Autorzy: Mahendran G , Muniappan V. , Ashwini M. , Muthukumar T. , Bai V. N.
• Języki publikacji: EN

Abstrakty

EN

An in vitro plant regeneration protocol was successfully established for Cymbidium bicolor an epiphytic orchid by culturing seeds from green pods. Immature seeds were germinated on four basal media viz., Murashige and Skoog (MS) medium, Knudson C (KC) orchid medium, Knudson C modified Morel (KCM) medium and Lindemann orchid (LO) medium. Seed germination and protocorm development was significantly higher in LO medium (96.6 %) followed by KCM (89 %), MS (77.5 %) and KC (62.7 %) media after 56 days. For multiple shoot induction the protocorms were transferred to B5 medium supplemented with cytokinin. Among the various cytokinins tested, BAP (4.42 lM) induced maximum (27.59) number of multiple shoots per explant. IBA was effective in inducing healthy roots. Tissue-cultured protocorms and seedlings of C. bicolor were inoculated with AC-01 fungal strain (Moniliopsis sp.) isolated from the mycorrizal roots of an epiphytic orchid Aerides crispum. Mycorrhizal fungi significantly enhanced number of roots, root length and shoot number.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2013
• Tom: 35
• Numer: 03
• Opis fizyczny: p.829-840,fig.,ref.

Twórcy

autor

Mahendran G

• Department of Botany, Plant Tissue culture lab, Bharathiar University, Coimbatore 641046, India

autor

Muniappan V.

• Department of Botany, School of Life Sciences, Bharathiar University, Coimbatore 641046, Tamilnadu, India

autor

Ashwini M.

• Department of Botany, School of Life Sciences, Bharathiar University, Coimbatore 641046, Tamilnadu, India

autor

Muthukumar T.

• Department of Botany, School of Life Sciences, Bharathiar University, Coimbatore 641046, Tamilnadu, India

autor

Bai V. N.

• Department of Botany, School of Life Sciences, Bharathiar University, Coimbatore 641046, Tamilnadu, India

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