Relevance of molecular changes in the ND4 gene in German Shepherd dog tumours

• Autorzy: Slaska B. , Grzybowska-Szatkowska L. , Bugno-Poniewierska M. , Gurgul A. , Smiech A. , Rozanska D. , Dudka J.
• Języki publikacji: EN

Abstrakty

EN

The aim of the study was to identify polymorphisms and mutations in the mitochondrial ND4 gene and to analyse the associations between the occurrence of molecular changes in mtDNA and phenotypic traits in tumours in German Shepherd dogs. Fifty samples obtained from blood and tumour tissues of German Shepherd dogs with diagnosed tumours were analysed. DNA extraction, amplification, and sequencing of the mtDNA ND4 gene, and bioinformatics, statistical, and in silico protein coding SNP analyses were performed. ND4 mutations and/or polymorphisms were noted in eleven nucleotide positions in nearly half of the examined dogs. All the changes were substitution mutations. A majority of the changes identified were homoplasmic. In one dog with osteosarcoma, blood heteroplasmy was detected. In two positions of the ND4 gene, presence of non-synonymous mutations leading to amino acid changes in the ND4 protein was reported. Analyses carried out to determine the deleterious effect of mutations indicated an almost 97 and 62% probability that a single amino acid substitution (p.G239V and p.I401T, respectively) in the protein has a negative impact on its function. The results of statistical analyses indicate a significant association between the occurrence of mutations in three loci of the ND4 gene and the location of tumours. The mutations identified may be a result of cell adaptation to the changes in the environment occurring during carcinogenesis. The high frequency of mutations in the tumours may indicate genetic instability of mtDNA, which may also play a role in carcinogenesis.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2016
• Tom: 19
• Numer: 3
• Opis fizyczny: p.461-469,fig.,ref.

Twórcy

autor

Slaska B.

• Department of Biological Bases of Animal Production, Faculty of Animal Breeding and Biology University of Life Sciences in Lublin, Akademicka 13, 20-950 Lublin, Poland

autor

Grzybowska-Szatkowska L.

• Department of Oncology, Medical University of Lublin, Jaczewskiego 7, 20-090 Lublin, Poland

autor

Bugno-Poniewierska M.

• Department of Genomics and Molecular Biology, National Research Institute of Animal Production, Krakowska 1, 32-083 Balice, Poland

autor

Gurgul A.

• Department of Genomics and Molecular Biology, National Research Institute of Animal Production, Krakowska 1, 32-083 Balice, Poland

autor

Smiech A.

• Department of Pathological Anatomy, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Gleboka 30, 20-612 Lublin, Poland

autor

Rozanska D.

• Department and Clinic of Animal Surgery, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Akademicka 13, 20-950 Lublin, Poland

autor

Dudka J.

• Independent Medical Biology Unit, Medical University of Lublin, Jaczewskiego 8b, 20-093 Lublin, Poland

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Identyfikatory

DOI

10.1515/pjvs-2016-0058