Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2013 | 35 | 07 |
Tytuł artykułu

Vacuolar invertases in potato (Solanum tuberosum L.):molecular cloning, characterization, sequence comparison, and analysis of gene expression in the cultivars

Warianty tytułu
Języki publikacji
In plants, vacuolar invertase (β-fructofuranosidase, EC is known to play as a key modulator for hexose accumulation and cell expansion. In this study, two cDNA clones (2,013 and 1,945 bp, with 99 % sequence identity) encoding vacuolar invertase isoforms were isolated from a commercially important Indian potato cultivar, Kufri Chipsona-1 by RT-PCR. The corresponding predicted proteins consisted of 635 amino acids (designated as KC-VIN1, lacking a few amino acids at N-terminus) and 639 amino acids (designated as KC-VIN2), respectively. They showed 99 % identity, and found to vary at several locations with mostly non-conservative substitutions. Multiple sequence alignment of vacuolar invertase homologs covering four Solanaceae family members revealed some notable distinguishing sequence features (signature-type sequences). A consensus sequence was predicted using 45 vacuolar invertase sequences from 27 taxonomically different plant species, and a phylogenetic tree was generated to know the evolutionary relation between them. Hydrophobic characters were predicted, and compared in different plant species. All these data are presented in a comprehensive manner which were not documented in the earlier reports. As a preliminary study, vacuolar invertase expression patterns in the tubers of some Indian potato cultivars were analyzed by semi-quantitative RT-PCR and extractable enzyme assay. In all the potato cultivars, the overall expression level of invertase was found to be considerably higher after storage at low temperature as compared to the freshly harvested tubers.
Słowa kluczowe
Opis fizyczny
  • Department of Biotechnology and Environmental Sciences, Thapar University, Patiala 147004, Punjab, India
  • Department of Biotechnology and Environmental Sciences, Thapar University, Patiala 147004, Punjab, India
  • Arai M, Mori H, Imaseki H (1992) Cloning and sequence of cDNAs for an intracellular acid invertase from etiolated hypocotyls of mung bean and expression of the gene during growth of seedlings. Plant Cell Physiol 33:245–252
  • Barratt DHP, Derbyshire P, Findlay K, Pike M, Wellner N, Lunn J, Feil R, Simpson C, Maule AJ, Smith AM (2009) Normal growth of Arabidopsis requires cytosolic invertase but not sucrose synthase. Proc Natl Acad Sci 106:13124–13129
  • Bhaskar PB, Wu L, Busse JS, Whitty BR, Hamernik AJ, Jansky SH, Buell CR, Bethke PC, Jiang J (2010) Suppression of the vacuolar invertase gene prevents cold-induced sweetening in potato. Plant Physiol 154:939–948
  • Corpet F (1988) Multiple sequence alignment with hierarchical clustering. Nucleic Acids Res 16:10881–10890
  • Davies C, Robinson SP (1996) Sugar accumulation in grape berries. Cloning of two putative vacuolar invertase cDNAs and their expression in grapevine tissues. Plant Physiol 111:275–283
  • Doolittle RF (1989) Redundancies in protein sequences. In: Fasman GD (ed) Prediction of protein structure and the principles of protein conformation. Plenum Press, New York, pp 599–623
  • Draffehn AM, Meller S, Li L, Gebhardt C (2010) Natural diversity of potato (Solanum tuberosum) invertases. BMC Plant Biol 10:271
  • Elliott KJ, Buttler WO, Dickinson CD, Konno Y, Vedvick TS, Fitzmaurice L, Mirkov TE (1993) Isolation and characterization of fruit vacuolar invertase genes from two tomato species and temporal differences in mRNA levels during fruit ripening. Plant Mol Biol 21:515–524
  • Fotopoulos V (2005) Plant invertases: structure, function and regulation of a diverse enzyme family. J Biol Res 4:127–137
  • Genova AD, Goverse A, Massa AN et al., The Potato Genome Consortium (2011) Genome sequence and analysis of the tuber crop potato. Nature 475:189-195
  • Gilman M (1987) Phenol/SDS method for plant RNA preparation. In: Ausubel FM et al (eds) Current protocols in molecular biology. John Wiley and Sons, New York, pp 431–434
  • Greiner S, Rausch T, Sonnewald U, Herbers K (1999) Ectopic expression of a tobacco invertase inhibitor homolog prevents cold-induced sweetening of potato tubers. Nat Biotech 17:708–711
  • Haouazine-Takvorian N, Tymowska-Lalanne Z, Takvorian A, Tregear J, Lejeune B, Lecharny A, Kreis M (1997) Characterisation of two members of the Arabidopsis thaliana gene family, Atbfruct3 and Atbfruct4, coding for vacuolar invertases. Gene 197:239–251
  • Hedley PE, Machray GC, Davies HV, Burch L, Waugh R (1993) cDNA cloning and expression of a potato (Solanum tuberosum) invertase. Plant Mol Biol 22:917–922
  • Huang WC, Wang AY, Wang LT, Sung HY (2003) Expression and characterization of sweet potato invertase in Pichia pastoris. J Agric Food Chem 51:1494–1499
  • Ji X, Van den Ende W, Van Laere A, Cheng S, Bennett J (2005) Structure, evolution, and expression of the two invertase gene families of rice. J Mol Evol 60:615–634
  • Kim JY, Mahe A, Brangeon J, Prioul JL (2000) A maize vacuolar invertase, IVR2, is induced by water stress. Organ/tissue specificity and diurnal modulation of expression. Plant Physiol 124:71–84
  • Koch K (2004) Sucrose metabolism: regulatory mechanisms and pivotal roles in sugar sensing and plant development. Curr Opin Plant Biol 7:235–246
  • Kyte J, Doolittle R (1982) A simple method for displaying the hydropathic character of a protein. J Mol Biol 157:105–132
  • Lammens W, Le Roy K, Schroeven L, Van Laere A, Rabijns A, Van den Ende W (2009) Structural insights into glycoside hydrolase family 32 and 68 enzymes: functional implications. J Exp Bot 60:727–740
  • Matsuura-Endo C, Kobayashi A, Noda T, Takigawa S, Yamauchi H, Mori M (2004) Changes in sugar content and activity of vacuolar acid invertase during low-temperature storage of potato tubers from six Japanese cultivars. J Plant Res 117:131–137
  • Menendez CM, Ritter E, Scha¨fer-Pregl R, Walkemeier B, Kalde A, Salamini F, Gebhardt C (2002) Cold sweetening in diploid potato: mapping quantitative trait loci and candidate genes. Genetics 162:1423–1434
  • Ohyama A, Hirai M, Nishimura S (1992) A novel cDNA clone for acid invertase in tomato fruit. Jpn J Genet 67:491–492
  • Ohyama A, Nishimura S, Hirai M (1998) Cloning of cDNA for a cell wall-bound acid invertase from tomato (Lycopersicon esculentum) and expression of soluble and cell wall-bound invertases in plants and wounded leaves of L. esculentum and L. peruvianum. Genes Genet Syst 73:149–157
  • Rausch T, Greiner S (2004) Plant protein inhibitors of invertases. Biochim Biophys Acta 1696:253–261
  • Richardson DL, Davies HV, Ross HA, Mackay GR (1990) Invertase activity and its relation to hexose accumulation in potato tubers. J Exp Bot 41:95–99
  • Rolland F, Baena-Gonzalez E, Sheen J (2006) Sugar sensing and signalling in plants: conserved and novel mechanisms. Ann Rev Plant Biol 57:675–709
  • Ruan Y-L, Jin Y, Yang Y-J, Li G-J, Boyer JS (2010) Sugar input, metabolism, and signaling mediated by invertase: roles in development, yield potential, and response to drought and heat. Mol Plant 3:942–955
  • Sadasivam S, Manickam A (1996) Biochemical methods. New Age International, New Delhi, India
  • Saitou N, Nei M (1987) The neighbor-joining method: A new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
  • Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, New York
  • Sato T, IwatsuboT Takahashi M, Nakagawa H, Ogura N, Mori H (1993) Intercellular localization of acid invertase in tomato fruit and molecular cloning of a cDNA for the enzyme. Plant Cell Physiol 34:263–269
  • Schäfer-Pregl R, Ritter E, Concilio L, Hesselbach J, Lovatti L, Walkemeier B, Thelen H, Salamini F, Gebhardt C (1998) Analysis of quantitative trait loci (QTLs) and quantitative trait alleles (QTAs) for potato tuber yield and starch content. Theor Appl Genet 97:834–846
  • Sherson SM, Alford HL, Forbes SM, Wallace G, Smith SM (2003) Roles of cell-wall invertases and monosaccharide transporters in the growth and development of Arabidopsis. J Exp Bot 54:525–531
  • Sturm A (1999) Invertases. Primary structures, functions, and roles in plant development and sucrose partitioning. Plant Physiol 121:1–7
  • Sturm A, Chrispeels MJ (1990) cDNA cloning of carrot extracellular [beta]-fructosidase and its expression in response to wounding and bacterial infection. Plant Cell 2:1107–1119
  • Sturm A, Hess D, Lee H-S, Lienhard S (1999) Neutral invertase is a novel type of sucrose-cleaving enzyme. Physiol Plant 107:159–165
  • Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739
  • Tian H, Kong Q, Feng Y, Yu X (2009) Cloning and characterization of a soluble acid invertase-encoding gene from muskmelon. Mol Biol Rep 36:611–617
  • Tymowska-Lalanne Z, Kreis M (1998) The plant invertases: physiology, biochemistry and molecular biology. Adv Bot Res 28:71–117
  • Unger C, Hardegger M, Lienhard S, Sturm A (1994) cDNA cloning of carrot (Daucus carota) soluble acid b-fructofuranosidases and comparison with the cell wall isoenzyme. Plant Physiol 104:1351–1357
  • van de Wal MHBJ, Jacobsen E, Visser RGF (2001) Multiple allelism as a control mechanism in metabolic pathways: GBSSI allelic composition affects the activity of granule-bound starch synthase I and starch composition in potato. Mol Genet Genomics 265:1011–1021
  • Van den Ende W, Coopman M, Clerens S, Vergauwen R, Le Roy K, Lammens W, Van Laere A (2011) Unexpected presence of graminan- and levan-type fructans in the evergreen frost-hardy eudicot Pachysandra terminalis (Buxaceae): purification, cloning, and functional analysis of a 6-SST/6-SFT enzyme. Plant Physiol 155:603–614
  • Verhaest M, Van den Ende W, Roy KL, De Ranter CJ, Laere AV, Rabijns A (2005) X-ray diffraction structure of a plant glycosyl hydrolase family 32 protein: fructan 1-exohydrolase IIa of Cichorium intybus. Plant J 41:400–411
  • Wang LT, Wang AY, Hsieh CW, Chen CY, Sung HY (2005) Vacuolar invertases in sweet potato: molecular cloning, characterization, and analysis of gene expression. J Agric Food Chem 53:3672–3678
  • Zhou D, Mattoo A, Li N, Imaseki H, Solomos T (1994) Complete nucleotide sequence of potato tuber acid invertase cDNA. Plant Physiol 106:397–398
  • Zrenner R, Schuler K, Sonnewald U (1996) Soluble acid invertase determines the hexose-to-sucrose ratio in cold-stored potato tubers. Planta 198:246–252
rekord w opracowaniu
Typ dokumentu
Identyfikator YADDA
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.