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2014 | 17 | 2 |

Tytuł artykułu

Seasonal-dependent variations in metabolic status of spermatozoa and antioxidant enzyme activity in the reproductive tract fluids of wild boar/domestic pig hybrids

Treść / Zawartość

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
This study investigated seasonal changes in the metabolic performance of spermatozoa and activity of the antioxidant enzymes in the seminal plasma of three wild boar/domestic pigs (aged 1.5 to 2.5 years) and the activity of the antioxidant enzymes in fluids of the cauda epididymidis and vesicular glands from 16 wild boar/domestic pig hybrids (aged 1 to 3 years). Parameters of the sperm metabolic activity, such as total motility, mitochondrial functions, and measurements of oxygen uptake, ATP content and L-lactate production, were analyzed during the spring-summer and autumn-winter periods. Besides these sperm metabolic parameters, the sperm membrane integrity was also assessed. Total protein content and activity of the antioxidant enzymes, such as superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx), were measured in the reproductive tract fluids. There were no marked significant differences (P > 0.05) between the seasonal periods in terms of sperm motility, mitochondrial function and oxygen uptake; however, spermatozoa collected during the autumn-winter period exhibited higher (P < 0.05) ATP content and L-lactate production than those harvested during the spring-summer period. It was found that the vesicular gland fluid exhibited a higher level of SOD activity during the spring-summer period compared with the autumn-winter period. Furthermore, CAT activity in the seminal plasma and vesicular gland fluid was greater during the autumn-winter. Total protein content was significantly higher in the vesicular gland fluid, whereas the cauda epididymidal fluid exhibited greater SOD and GPx activities, irrespective of the seasonal period. The findings of this study confirmed seasonal-related differences in the metabolic performance of spermatozoa and activity of antioxidant enzymes of the reproductive tract of the boar/domestic pig hybrids.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

17

Numer

2

Opis fizyczny

p.307-313,ref.

Twórcy

  • Department of Animal Biochemistry and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 5, 10-718 Olsztyn-Kortowo, Poland
autor
  • Department of Animal Biochemistry and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 5, 10-718 Olsztyn-Kortowo, Poland
  • Department of Animal Biochemistry and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 5, 10-718 Olsztyn-Kortowo, Poland
autor
  • Department of Animal Biochemistry and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 5, 10-718 Olsztyn-Kortowo, Poland
  • Branch Campus, Faculty of Biotechnology, University of Rzeszow, Werynia 502, 36-100 Kolbuszowa, Poland
autor
  • Department of Animal Biochemistry and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 5, 10-718 Olsztyn-Kortowo, Poland

Bibliografia

  • Aitken RJ, Vernet P (1998) Maturation of redox regulatory mechanisms in the epididymis. J Reprod Fertil Suppl 53: 109-118.
  • Beauchamp C, Fridovich I (1971) Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal Biochem 44: 276-287.
  • Claus R, Weiler U (1985) Influence of light and photoperiodicity on pig prolificacy. J Reprod Fert Suppl 33: 185-197.
  • Claus R, Weiler U, Wagner HG (1985) Photoperiodic influences on reproduction of domestic boars. II. Light influences on semen characteristics and libido. Zentralbl Veterinarmed A 32: 99-109.
  • Ciereszko A, Ottobre JS, Glogowski J (2000) Effects of season and breed on sperm acrosin activity and semen quality of boars. Anim Reprod Sci 64: 89-96.
  • Dziekońska A, Fraser L, Strzezek J (2009) Effect of different storage temperatures on the metabolic activity of spermatozoa following liquid storage of boar semen. J Anim Feed Sci 18: 638-649.
  • Fraser L, Gorszczaruk K, Lecewicz M, Strzezek J (2003) Age-related changes and seasonal variations in boar sperm metabolism during liquid storage at 5 and 16 deg C. J Anim Feed Sci 12: 803-811.
  • Fraser L, Lecewicz M, Strzezek J (2002) Fluorometric assessments of viability and mitochondrial status of boar spermatozoa following liquid storage. Pol J Vet Sci 5: 85-92.
  • Halangk W, Bohnensack R, Kunz W (1985) Interdependence of mitochondrial ATP production and extramitochondrial ATP utilization in intact spermatozoa. Biochim Biophys Acta 808: 316-322.
  • Jones AR (1997) Metabolism of lactate by mature boar spermatozoa. Reprod Fertil Dev 9: 227-232.
  • Klimas R, Klimiene A, Pileckas V (2012) Seasonal effect on sperm morphological properties of European wild boar (Sus scrofa L) in Lithuania. Vet Med Zoot 58: 20-25.
  • Kowalowka M, Wysocki P, Fraser L, Strzeżek J (2008) Extracellular superoxide dismutase of boar seminal plasma. Reprod Domest Anim 43: 490-496.
  • Kondracki S, Antolik A, Zwierz B (1997) Characters of boar semen depending on the season of the year. Roczniki Naukowe Zootechniki 24: 67-76.
  • Kozdrowski R, Dubiel A (2004a) The effect of season on the properties of wild boar (Sus scrofa L) semen. Anim Reprod Sci 80: 281-289.
  • Kozdrowski R, Dubiel A (2004b) Properties of semen in the annual cycle of wild boar/domestic pig hybrids. Med Weter 60: 57-61.
  • Koziorowska-Gilun M, Koziorowski M, Fraser L, Strzeżek J (2011a) Antioxidant defence system of boar cauda epididymidal spermatozoa and reproductive tract fluids. Reprod Domest Anim 46: 527-533.
  • Koziorowska-Gilun M, Koziorowski M, Strzezek J, Fraser L (2011b) Seasonal changes in antioxidant defence systems in seminal plasma and fluids of the boar reproductive tract. Reprod Biol 11: 37-47.
  • Mauget R, Boissin J (1987) Seasonal changes in testis weight and testosterone concentration in the European wild boar (Sus scrofa L). Anim Reprod Sci 13: 67-74.
  • Mruk DD, Silvestrini B, Mo MY, Cheng CY (2002) Antioxidant superoxide dismutase - a review: its function, regulation in the testis, and role in male fertility. Contraception 65: 305-311.
  • Peeker R, Abramsson L, Marklund SL (1997) Superoxide dismutase isoenzymes in human seminal plasma and spermatozoa. Mol Hum Reprod 3: 1061-1066.
  • Peltoniemi OA, Tast A, Love RJ (2000) Factors effecting reproduction in the pig: seasonal effects and restricted feeding of the pregnant gilt and sow. Animl Reprod Sci 60-61: 173-184.
  • Rodriguez-Gil JE, Rigau T (1995) Effects of slight agitation on the quality of refrigerated boar sperm. Anim Reprod Sci 39: 141-146.
  • Rigau T, Piedrafita J, Reverter A, Canal M, Rodriguez-Gil JE (1996) The rate of L-lactate production: a feasiable parameter for the fresh diluted boar semen quality analysis. Anim Reprod Sci 43: 161-172.
  • Sancho S, Pinart E, Briz M, Garcia-Gil N, Badia E, Bassols J, Khdhr E, Pruneda A, Bussalleu E, Yeste M, Coll MG, Bonet S (2004) Semen quality of postpubertal boars during increasing and decreasing natural photoperiods. Theriogenology 62: 1271-1282.
  • Skrzycki M, Majewska M, Podsiad M, Czeczot H (2009) Expression and activity of superoxide dismutase isoenzymes in colorectal cancer. Acta Biochim Pol 56: 663-670.
  • Strzezek J (2002) Secretory activity of boar seminal vesicle glands. Reprod Biol 2: 243-266.
  • Strzezek J, Fraser L, Demianowicz W, Kordan W, Wysocki P, Hołody D (2000) Effect of depletion tests (DT) on the composition of boar semen. Theriogenology 54: 949-963.
  • Thomas CA, Garner DL, DeJarnette JM, Marshall CE (1998) Effect of cryopreservation of bovine sperm organelle function and viability as determined by flow cytometry. Biol Reprod 58: 786-793.
  • Weichselbaum TE (1946) An accurate and rapid method for the determination of proteins in small amounts of blood serum and plasma. Am J Clin Pathol 10: 40-49.
  • Zini A, Schlegel PN (1996) Catalase mRNA expression in the male rat reproductive tract. J Androl 17: 473-480.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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