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2008 | 52 | 4 |

Tytuł artykułu

Determination of riboflavin in premixture and compound feed by liquid chromatography method

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
The analytical procedure of riboflavin quantification in premixtures and compound feedingstuffs by high performance liquid chromatography (HPLC) analysis is presented. Riboflavin was extracted from feed sample in autoclave with 0.1M sulphuric acid. The ester bonds with phosphoric acid were hydrolysed by the Taka-diastase enzyme. Riboflavin content was determined by HPLC with reversed-phase and usage of fluorescence detector. The limit of quantification of this method is 1.0 mg/kg. The coefficient of variation of riboflavin quantification results in premixtures was 2.29% and in compound feedingstuffs - 3.39%. The Horrat value for premixtures reached 0.61, whereas in case of compound feedingstuffs - 0.40, thus confirming an acceptable precision of the applied procedure. The recovery rate for riboflavin added in the form of a reference solution into premixtures was 98.3%, as regards compound feedingstuffs it was 98.0%.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

52

Numer

4

Opis fizyczny

p.619-624,fig.,ref.

Twórcy

autor
  • National Feed Laboratory, National Research Institute of Animal Production, 20-079 Lublin, Poland
autor
  • National Feed Laboratory, National Research Institute of Animal Production, 20-079 Lublin, Poland
autor
  • National Feed Laboratory, National Research Institute of Animal Production, 20-079 Lublin, Poland
autor
  • National Veterinary Research Institute, 24-100 Pulawy, Poland

Bibliografia

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  • 2. Anonimus: Analytical Methods Committee. Determination of thiamine and riboflavin in pet foods and animal feedingstuffs. Analyst 2000, 125, 353-360.
  • 3. Britton N.L., Riter K.L., Smallidge R.L., Hillebrandt J.: Reversed-phase liquid chromatographic determination of riboflavin in feeds. J AOAC Int 2003, 86, 197-201.
  • 4. Eitenmiller R.R., Lin Ye, Landen W.O., Jr.: Riboflavin. In: Vitamin Analysis for the Health and Food Sciences. CRC Press, Boca Raton, FL, 2008, pp. 291-324.
  • 5. Finglas P.M., Faulks R.M.: Critical review of HPLC method for the determination of thiamine, riboflavin and niacin in food. J Micronutr Anal 1987, 3, 251-283.
  • 6. Eurolab Technical Report 1/2007. Measurement uncertainty revisited: Alternative approaches to uncertainty evaluation, Eurolab, Paris, 2007.
  • 7. Handbook for Calculation of Measurement Uncertainty in Environmental Laboratories. Nordtest Project 1589-02. 2007. www.nordicinnovation.net
  • 8. Horwitz W.: Evaluation of analytical methods used for regulation of food and drugs. Anal Chem 1982, 54, 67A-76A.
  • 9. Horwitz W. (Editor). Official Methods of Analysis of AOAC International, 17th Edition. AOAC International, Gaithersburg, USA, 2000.
  • 10. Keller H.E.: Analytical Methods for Vitamins and Carotenoids in Feed, Department of Vitamin Reaserch and Development Roche Basle, 1988.
  • 11. Nielsen P.: Flavins. In: Modern Chromatographic Analysis of Vitamins. Edited by A.P. DeLeenheer, W.L. Lambert, H.J. Nelis, Dekker, New York, 1992, p. 355.
  • 12. Nutrient Recommendation and Nutritive Value of Feedingstuffs for Poultry. Poultry Feeding Standards (in Polish). Edited by S. Smulikowska and A. Rutkowski, Institute of Physiology and Animal Feeding, Jabłonna, 2005.
  • 13. Raw Material Compendium. A compilation of worldwide data sources. Novus Europe S.A. Brussels, 1996, p. 541.
  • 14. Rubaj J., Korol W., Bielecka G., Kwiatek K.: Determination of thiamine in premixture and compound feed by liquid chromatography method. Bull Vet Inst Pulawy 2008, 52, 435-440.
  • 15. Russell L.F., Vanderslice J.T.: A comprehensive review of vitamin B₂ analytical methodology. J Micronutr Anal 1990, 8, 257-310.
  • 16. Swine Feeding Standards. Nutritive Value of Feedingstuffs (in Polish). Institute of Physiology and Animal Feeding, Jabłonna, 1993.
  • 17. Van der Poel A.F.B., Gadient M.: Effects of expander processing on the recovery of feed additives. In: Expander Processing of Animal Feeds. Edited by A.F.B Van der Poel. Feed Processing Centre, Wageningen, 1997, pp. 49-56.
  • 18. Woollard D., Indyk H.E.: Rapid determination of thiamine, riboflavin, pyridoxine and niacinamide in infant formulas by liquid chromatography. J AOAC Int 2002, 85, 945-951.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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