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2019 | 79 | 2 |
Tytuł artykułu

Short‑term exposure to high‑intensity sound induces hearing loss and apoptosis in guinea pigs

Warianty tytułu
Języki publikacji
The aim of this study was to investigate the effect and underlying mechanisms of short-term high-intensity sound exposure on guinea pigs to mimic the effects of non-lethal anti-riot weapons. A total of 92 male adult guinea pigs were exposed to high-intensity sound at 0 dB, 110 dB and 130 dB for 5 min. Basic clinical observation, repellent behaviour detection, peripheral blood routine examination, serum cortisol detection and hearing ability assessment were performed to analyse the functional changes after high intensity sound exposure. Meanwhile, routine haematoxylin and eosin staining, scanning electron microscopy and transmission electron microscopy were used to observe the structure of the cochlear tissue. To investigate the mechanisms underlying the tissue changes, the levels of apoptosis and caspase 3, 8 and 9 were detected using TUNEL staining and immunohistochemistry. After short-term exposure to high-intensity sound, the guinea pigs exhibited fear and agitation, increased repulsive behaviour, high serum cortisol and an increase in auditory threshold. The inner hair cells and outer hair cells exhibited degeneration. In addition, apoptosis was observed in the cochlear tissue. After short-term exposure to high-intensity sound, the guinea pigs exhibited not only stress reactions but also impaired hearing and signs of hair cell degeneration. Apoptosis in the cochlear tissue may play an important role in the functional and structural injuries caused by high-intensity sound.
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Opis fizyczny
  • Institute of Disaster Medicine, Tianjin University, Tianjin, China
  • Department of Medical Service, Logistics University of Chinese People’s Armed Police Forces, Tianjin, China
  • Department of Experimental Pathology, Institute of Radiation Medicine, Academy of Military Sciences, Beijing, China
  • Institute of Disaster Medicine, Tianjin University, Tianjin, China
  • Department of Medical Service, Logistics University of Chinese People’s Armed Police Forces, Tianjin, China
  • Institute of Disaster Medicine, Tianjin University, Tianjin, China
  • Institute of Disaster Medicine, Tianjin University, Tianjin, China
  • Institute of Disaster Medicine, Tianjin University, Tianjin, China
  • Adler HJ, Kenealy JF, Dedio RM, Saunders JC (1992) Threshold shift, hair cell loss, and hair bundle stiffness following exposure to 120 and 125 dB pure tones in the neonatal chick. Acta Otolaryngol 112: 444–454.
  • Akdogan O, Selcuk A, Take G, Erdogan D, Dere H (2009) Continuous or intermittent noise exposure, does it cause vestibular damage? An experimental study. Auris Nasus Larynx 361: 2–6.
  • Carrasco GA, Van de Kar LD (2003) Neuroendocrine pharmacology of stress. Eur J Pharmacol 463: 235–272.
  • Chen GD, Decker B, Krishnan Muthaiah VP, Sheppard A, Salvi R (2014) Prolonged noise exposure-induced auditory threshold shifts in rats. Hear Res 317: 1–8.
  • Fernandez KA, Jeffers PW, Lall K, Liberman MC, Kujawa SG (2015) Aging after noise exposure: acceleration of cochlear synaptopathy in “recovered” ears. J Neurosci 35: 7509–7520.
  • Fouladi DB, Nassiri P, Monazzam EM, Farahani S, Hassanzadeh G, Hoseini M (2012) Industrial noise exposure and salivary cortisol in blue collar industrial workers. Noise Health 14: 184–189.
  • Han W J, Shi XR, Nuttall A (2007) Caspase 3 activation and apoptosis inducing factor translocation in noise exposure induced out hair cells apoptosis (in Chinese). Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 42: 515–519.
  • Han WJ, Shi XR, Nuttall A (2013) Noise-induced nitrotyrosine increase and outer hair cell death in guinea pig cochlea. Chin Med J 126: 2923–2927.
  • Heeringa AN , van Dijk P (2014) The dissimilar time course of temporary threshold shifts and reduction of inhibition in the inferior colliculus following intense sound exposure. Hear Res 312: 38–47.
  • Heinrich UR, Selivanova O, Schmidtmann I, Feltens R, Brieger J, Mann WJ (2010) Noise exposure alters cyclooxygenase 1 (COX-1) and 5-lipoxygenase (5-LO) expression in the guinea pig cochlea. Acta Otolaryngol 130: 358–365.
  • Hu BH, Guo W, Wang PY, Henderson D, Jiang SC (2000) Intense noise-induced apoptosis in hair cells of guinea pig cochleae. Acta Otolaryngol 120: 19–24.
  • Hu BH, Henderson D, Nicotera TM (2002) Involvement of apoptosis in progression of cochlear lesion following exposure to intense noise. Hear Res 166: 62–71.
  • Hu BH (2007) Delayed mitochondrial dysfunction in apoptotic hair cells in chinchilla cochleae following exposure to impulse noise. Apoptosis 12: 1025–1036.
  • Ide M, Morimitsu T (1990) Long term effects of intense sound on endocochlear DC potential. Auris Nasus Larynx 17: 1–10. Jacob S, Johansson C, Fridberger A (2013) Noise-induced alterations in cochlear mechanics, electromotility, and cochlear amplification. Pflugers Arch 465: 907–917.
  • Jensen JB, Lysaght AC, Liberman MC, Qvortrup K, Stankovic KM (2015) Immediate and delayed cochlear neuropathy after noise exposure in pubescent mice. PLoS One 10: e0125160.
  • Kominami K, Nakabayashi J, Nagai T, Tsujimura Y, Chiba K, Kimura H, Miyawaki A, Sawasaki T, Yokota H, Manabe N, Sakamaki K (2012) The molecular mechanism of apoptosis upon caspase-8 activation: quantitative experimental validation of a mathematical model. Biochim Biophys Acta 1823: 1825–1840.
  • Liberman LD, Suzuki J, Liberman MC (2015) Dynamics of cochlear synaptopathy after acoustic overexposure. J Assoc Res Otolaryngol 16: 205–219.
  • Lo WC, Liao LJ, Wang, CT, Young, YH, Chang YL, Cheng PW (2013) Dose-dependent effects of D-methionine for rescuing noise-induced permanent threshold shift in guinea-pigs. Neuroscience 254: 222–229.
  • Maison SF, Usubuchi H, Liberman MC (2013) Efferent feedback minimizes cochlear neuropathy from moderate noise exposure. J Neurosci 33: 5542–5552.
  • Majno G, Joris I (1995) Apoptosis, oncosis, and necrosis. An overview of cell death. Am J Pathol 146: 3–15.
  • Matsunobu T, Satoh Y, Ogawa, K, Shiotani A (2009) Heme oxygenase-1 expression in the guinea pig cochlea induced by intense noise stimulation. Acta Otolaryngol Suppl 562: 18–23.
  • Miller GE, Chen E, Zhou ES (2007) If it goes up, must it come down? Chronic stress and the hypothalamic-pituitary-adrenocortical axis in humans. Psychol Bull 133: 25–45.
  • Muchnik C, Rosenthal T, Peleg E, Hildesheimer M (1998) Stress reaction to intense sound exposure under different arousal levels in guinea pigs. Acta Otolaryngol 118: 646–650.
  • Pawlaczyk-Luszczynska M, Dudarewicz A, Zamojska M, Sliwinska-Kowalska M (2011) Evaluation of sound exposure and risk of hearing impairment in orchestral musicians. Int J Occup Saf Ergon 17: 255–269.
  • Saedi B, Ghasemi M, Motiee M, Mojtahed M, Safavi A (2013) Transient threshold shift after gunshot noise exposure. B-ENT 9: 133–139.
  • Sergeyenko Y, Lall K, Liberman MC, Kujawa SG (2013) Age-related cochlear synaptopathy: an early-onset contributor to auditory functional decline. J Neurosci 33: 13686–13694.
  • Shalini S, Dorstyn L, Dawar S, Kumar S (2015) Old, new and emerging functions of caspases. Cell Death Differ 22: 526–539.
  • Shi L, Liu L, He T, Guo X, Yu Z, Yin S, Wang J (2013) Ribbon synapse plasticity in the cochleae of Guinea pigs after noise-induced silent damage. PLoS One 8: e81566.
  • Shi Y (2002) Mechanisms of caspase activation and inhibition during apoptosis. Mol Cell 9: 459–470.
  • Shoji F, Yamasoba T, Magal E, Dolan DF, Altschuler RA, Miller JM (2000) Glial cell line-derived neurotrophic factor has a dose dependent influence on noise-induced hearing loss in the guinea pig cochlea. Hear Res 142: 41–55.
  • Wang B, Liu Y, Chi F, Zhang Y, Yang M, Zhu X (2013) Dexamethasone suppresses cochlear Hes1 expression after noise exposure. Acta Otolaryngol 133: 233–238.
  • Wang F, Zuo L, Hong B, Han D, Range EM, Zhao L, Sui Y, Guo W, Liu L (2013) Tonotopic reorganization and spontaneous firing in inferior colliculus during both short and lo ng recovery periods after noise overexposure. J Biomed Sci 20: 91.
  • Zhang B, Zhou, HS, Cheng Q, Lei L, Hu B (2014) Low-frequency ultrasound induces apoptosis of rat aortic smooth muscle cells (A7r5) via the intrinsic apoptotic pathway. Genet Mol Res 13: 3143–3153.
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