Effects of Lactococus lactis producing myelin peptides on histopathological changes in spinal cord of rats with Experimental Allergic Encephalomyelitis

• Autorzy: Kasarello K. , Szczepankowska A. K. , Patzer-Kwiatkowska R. , Bardowski J. , Lipkowski A. W.
• Języki publikacji: EN

Abstrakty

EN

Experimental Allergic Encephalomyelitis (EAE) is the animal model of Multiple Sclerosis (MS), human autoimmunological disease that causes neurodegeneration. The autoimmune base of the disease leads to treatment searching in immunological mechanisms. Earlier we proposed the application of pig spinal cord hydrolysate as a mean to induce oral tolerance. The positive effects observed in EAE rats, stimulated us to develop bacteria that may express active peptide related to myelin fragment. For our experiments we used mixture of Lactococcus lactis producing fragments of three main myelin peptides, MBP (MBP 85-97), PLP (PLP 139-151) and MOG (MOG 35-55). We fed female Lewis rats with spectrum of bacteria doses, from 101 to 108 cells/ rat daily, for twenty days, from day −10 to 9. At the day 0 we evoked EAE in rats. Based on the results obtained from clinical symptoms, we selected two doses, 103 and 106 bacteria cells/rat for investigation of histopathological changes in spinal cord of animals. We observed slighter inflammatory cells infiltration in spinal cord in EAE rats fed with both doses of bacteria in comparison to non-fed ones. Supported by N302 00932/1139 grant.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2013
• Tom: 73
• Numer: Suppl.1
• Opis fizyczny: p.54

Twórcy

autor

Kasarello K.

• Mossakowski Medical Research Centre, Polish Academy of Science, Warsaw, Poland

autor

Szczepankowska A. K.

• Institute of Biochemistry and Biophysics, Polish Academy of Science, Warsaw, Poland

autor

Patzer-Kwiatkowska R.

• Mossakowski Medical Research Centre, Polish Academy of Science, Warsaw, Poland

autor

Bardowski J.

• Institute of Biochemistry and Biophysics, Polish Academy of Science, Warsaw, Poland

autor

Lipkowski A. W.

• Mossakowski Medical Research Centre, Polish Academy of Science, Warsaw, Poland