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2002 | 07 | 2A |

Tytuł artykułu

The use of molecular markers in apple breeding for disease resistance

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Molecular markers have become a useful tool simplifying and speeding up breeding work. They are also helpful in the genetic analysis of complex agronomic traits. The investigations concern the use of already available SCAR markers for molecular analysis of breeding materials. The markers are the apple powdery mildew resistance gene Pl2, originating from Malus zumi, and the apple scab resistance gene Vf, from M. floribunda 821. The clone U 211 was found to be highly resistant to mildew under field conditions and transmitted a high level of resistance to the majority of its progeny. The presence of the Vf gene conferring resistance to scab was confirmed by molecular analysis. As the molecular markers for the Pl2 gene are not present in the DNA of U 211, it is probable that the clone U 211 is a new source of mildew resistance.

Wydawca

-

Rocznik

Tom

07

Numer

2A

Opis fizyczny

p.445-448,fig.,ref.

Twórcy

  • Department of Pomology and Basic Natural Sciences in Horticulture, Warsaw Agricultural University – SGGW, Nowoursynowska 166, 02-787 Warsaw, Poland
autor
  • Department of Pomology and Basic Natural Sciences in Horticulture, Warsaw Agricultural University – SGGW, Nowoursynowska 166, 02-787 Warsaw, Poland
  • Department of Pomology and Basic Natural Sciences in Horticulture, Warsaw Agricultural University – SGGW, Nowoursynowska 166, 02-787 Warsaw, Poland

Bibliografia

  • 1. Pitera, E. and Bogdanowicz, J. Evaluation of scab-resistant apple progenies for powdery mildew tolerance. Acta Hort. 317 (1992) 81-87.
  • 2. Rogers, S. O. and Bendich, A. J. Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues. Plant Mol. Biol. 5 (1985) 69-76.
  • 3. Gardiner, S., Bus, V., Basset, H., Goodman, M., Greer, L., Ranatunga, C., Rikkerink, E. and Forster, R. Identification of molecular markers linked to major resistances to apple scab, powdery mildew and wooly aphid in apple. PAG VII Conference, San Diego, CA, 1999, Abstr. 185.
  • 4. Tartarini, S., Gianfranceschi, L., Sansavini, S. and Gessler, C. Development of reliable PCR markers for the selection of the Vf gene conferring scab resistance in apple. Plant Breed. 118 (1999) 183-186.
  • 5. Markussen, T., Kruger, J., Schmidt, H. and Dunemann, F. Identification of PCR-based markers linked to the powdery-mildew-resistance gene P11 from Malus robusta. Plant Breed. 114 (1995) 530-534.
  • 6. Stankiewicz, M., Pitera, E. and Gawronski S. W. Genetic analysis of a Polish 3 apple selection U 211 as a new source of high resistance to apple powdery mildew. Acta Hort. 546 (2001) 641-644.
  • 7. Gardiner, S., Bus, V., Bassett, H., White, A., Noiton, D., Rikkerink, E., Ball, R., and Forster, R. An updated genetic map around the Vf gene for resistance to apple scab and marker assisted selection for resistance. Acta Hort. 484 (1999) 481-485.

Typ dokumentu

Bibliografia

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