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2019 | 59 | 2 |

Tytuł artykułu

The toxicity of Cry1Ia5 transgenic soybean plants against Spodoptera littoralis

Treść / Zawartość

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Three transgenic soybean lines expressing the Cry1Ia5 gene were developed using the Agrobacterium transformation system. The integration of the Cry1Ia5 gene in the genome of the transgenic plants was approved using specific primers for PCR and real time PCR analysis, respectively. The insecticidal activity of three transgenic lines (L1, L2 and L3) against 2nd larval instars Spodoptera littoralis was tested. The data indicate that L2 exhibited the highest mortality percentage 9 days post feeding (60%) followed by L3 (40%) then L1 (20%) while the control showed 0% mortality. The larvae fed transgenic material appeared smaller in size than compared to the control larvae. The reduction in insect size and weight was due to the accumulation of higher phenoloxidase activity in insect tissues. The higher mortality observed in L2 was due to a significant decrease in the acetylcholine esterase activity that leads to accumulation of acetylcholin at higher levels which causes paralysis and death. The developed transgenic line 2 could be used to construct an insect resistant soybean cultivar.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

59

Numer

2

Opis fizyczny

p.185-192,fig.,ref.

Twórcy

  • Department of Genetics, Faculty of Agriculture, Cairo University, Giza, Egypt
  • Department of Field Crop Pests, Plant Protection Research Institute, Agriculture Research Center, Dokki, Egypt
autor
  • Department of Field Crop Pests, Plant Protection Research Institute, Agriculture Research Center, Dokki, Egypt

Bibliografia

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Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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