A new method for analyzing gene expression based on frequency analysis of RT-PCR products obtained with degenerate primers

• Autorzy: Kiselev K.V. , Dubrovina A. S.
• Języki publikacji: EN

Abstrakty

EN

This paper presents a new method of gene expression analysis: frequency analysis of RT-PCR products obtained with degenerate primers (FAPP). The main advantage of the new approach compared to the present methods of gene expression analysis is that it is applicable to non-model plant objects whose gene sequences are unknown. The advantages and disadvantages of FAPP are described in detail using data on calcium-dependent protein kinase, stilbene synthase, and phenylalanine ammonialyase gene expression in two cell cultures of Vitis amurensis. We compared the expression profiles obtained by FAPP to those obtained by real-time PCR and expressed sequence tags.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2010
• Tom: 32
• Numer: 3
• Opis fizyczny: p.495-502,fig.,ref.

Twórcy

autor

Kiselev K.V.

• Laboratory of Biotechnology, Institute of Biology and Soil Science, Far East Branch of Russian Academy of Sciences, Stoletija Str. 159, 690022 Vladivostok, Russia

autor

Dubrovina A. S.

• Laboratory of Biotechnology, Institute of Biology and Soil Science, Far East Branch of Russian Academy of Sciences, Stoletija Str. 159, 690022 Vladivostok, Russia

Bibliografia

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