Staphylococcal cassette chromosome mec (SCCmec) classification and typing methods: an overview

• Autorzy: Turlej A. , Hryniewicz W. , Empel J.
• Języki publikacji: EN

Abstrakty

Meticillin-resistant Staphylococcus aureus (MRSA) is one of the main causes of hospital-acquired infections, but since late 1990s also the community-acquired. For better understanding of the S. aureus epidemiology there is an urgent need for creation of new typing method for SCCmec element. The molecular typing of MRSA for epidemiological purposes is investigated by pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), spa typing and the SCCmec type assignment. In last few years not only new type of SCCmec (VI to XI) have been identified, but also additional subtypes (i.e. IVg-j) and different variants of already existed one (i.e. 5C2&5 and 2B2&5) were discovered. The aim of this review is to briefly summarize current knowledge about SCCmec classification and to discuss advantages and disadvantages of selected SCCmec typing methods.

Słowa kluczowe

EN

Staphylococcus aureus; meticillin zob.methicillin; methicillin; resistance; bacterial infection; chromosome; SCCmec classification; SCCmec typing method

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2011
• Tom: 60
• Numer: 2
• Opis fizyczny: p.95-103,ref.

Twórcy

autor

Turlej A.

• National Medicine Institute, Chelmska 30/34, 00-725 Warsaw, Poland

autor

Hryniewicz W.

autor

Empel J.

Bibliografia

• Aires de Sousa M. and H. de Lencastre. 2004. Bridges from hospitals to the laboratory: genetic portraits of methicillin-resistant Staphylococcus aureus clones. FEMS Immunol. Med. Microbiol. 40: 101–111.
• Berger-Bachi B. and S. Rohrer. 2002. Factors influencing methicillin resistance in staphylococci. Arch. Microbiol. 178: 165–171.
• Berglund C., T. Ito, M. Ikeda, X.X. Ma, B. Soderquist and K. Hiramatsu. 2008. Novel type of staphylococcal cassette chromosome mec in a methicillin-resistant Staphylococcus aureus strain isolated in Sweden. Antimicrob. Agents. Chemother. 52: 3512–3516.
• Berglund C., T. Ito, X.X. Ma, M. Ikeda, S. Watanabe, B. Soderquist and K. Hiramatsu. 2009. Genetic diversity of methicillin-resistant Staphylococcus aureus carrying type IV SCCmec in Orebro County and the western region of Sweden. J. Antimicrob. Chemother. 63: 32–41.
• Boye K., M.D. Bartels, I.S. Andersen, J.A. Moller and H. Westh. 2007. A new multiplex PCR for easy screening of methicillin-resistant Staphylococcus aureus SCCmec types I–V. Clin. Microbiol. Infect. 13: 725–727.
• Boyle-Vavra S., B. Ereshefsky, C.C. Wang and R.S. Daum. 2005. Successful multiresistant community-associated methicillin-resistant Staphylococcus aureus lineage from Taipei, Taiwan, that carries either the novel staphylococcal chromosome cassette mec (SCCmec) type VT or SCCmec type IV. J. Clin. Microbiol. 43: 4719–4730.
• Cha H.Y., D.C. Moon, C.H. Choi et al. 2005. Prevalence of the ST239 clone of methicillin-resistant Staphylococcus aureus and differences in antimicrobial susceptibilities of ST239 and ST5 clones identified in a Korean hospital. J. Clin. Microbiol. 43: 3610–3614.
• Chambers H.F. and F.R. Deleo. 2009. Waves of resistance: Staphylococcus aureus in the antibiotic era. Nat. Rev. Microbiol. 7: 629–641.
• Chen L., J.R. Mediavilla, D.C. Oliveira, B.M. Willey, H. de Lencastre and B.N. Kreiswirth. 2009. Multiplex real-time PCR for rapid staphylococcal cassette chromosome mec typing. J. Clin. Microbiol. 47: 3692–3706.
• Chlebowicz M.A., K. Nganou, S. Kozytska, J.P. Arends, S. Engelmann, H. Grundmann, K. Ohlsen, J.M. van Dijl and G. Buist. 2010. Recombination between ccrC genes in a type V (5C2&5) staphylococcal cassette chromosome mec (SCCmec) of Staphylococcus aureus ST398 leads to conversion from methicillin resistance to methicillin susceptibility in vivo. Antimicrob. Agents. Chemother. 54: 783–791.
• Chongtrakool P., T. Ito, X.X. Ma et al. 2006. Staphylococcal cassette chromosome mec (SCCmec) typing of methicillin-resistant Staphylococcus aureus strains isolated in 11 Asian countries: a proposal for a new nomenclature for SCCmec elements. Antimicrob. Agents. Chemother. 50: 1001–1012.
• Deurenberg R.H., C. Vink, S. Kalenic, A.W. Friedrich, C.A. Bruggeman and E.E. Stobberingh. 2007. The molecular evolution of methicillin-resistant Staphylococcus aureus. Clin. Microbiol. Infect. 13: 222–235.
• Deurenberg R.H. and E.E. Stobberingh. 2008. The evolution of Staphylococcus aureus. Infect. Genet. Evol. 8: 747–763.
• Francois P., G. Renzi, D. Pittet, M. Bento, D. Lew, S. Harbarth, P. Vaudaux and J. Schrenzel. 2004. A novel multiplex real-time PCR assay for rapid typing of major staphylococcal cassette chromosome mec elements. J. Clin. Microbiol. 42: 3309–3312.
• Heusser R., M. Ender, B. Berger-Bachi and N. McCallum. 2007. Mosaic staphylococcal cassette chromosome mec containing two recombinase loci and a new mec complex, B2. Antimicrob. Agents. Chemother. 51: 390–393.
• Higuchi W., T. Takano, L.J. Teng. and T. Yamamoto. 2008. Structure and specific detection of staphylococcal cassette chromosome mec type VII. Biochem. Biophys. Res. Commun. 377: 752–756.
• Hiramatsu K., L. Cui, M. Kuroda and T. Ito. 2001. The emergence and evolution of methicillin-resistant Staphylococcus aureus. Trends. in microbiology 9: 486–493.
• Hiramatsu K., Y. Katayama, H. Yuzawa and T. Ito. 2002. Molecular genetics of methicillin-resistant Staphylococcus aureus. Int. J. Med. Microbiol. 292: 67–74.
• Hisata K., K. Kuwahara-Arai, M. Yamanoto et al. 2005. Dissemination of methicillin-resistant staphylococci among healthy Japanese children. J. Clin. Microbiol. 43: 3364–3372.
• Ito T., Y. Katayama and K. Hiramatsu. 1999. Cloning and nucleotide sequence determination of the entire mec DNA of pre-methicillin-resistant Staphylococcus aureus N315. Antimicrob. Agents. Chemother. 43: 1449–1458.
• Ito T., Y. Katayama, K. Asada, N. Mori, K. Tsutsumimoto, C. Tiensasitorn and K. Hiramatsu. 2001. Structural comparison of three types of staphylococcal cassette chromosome mec integrated in the chromosome in methicillin-resistant Staphylococcus aureus. Antimicrob. Agents. Chemother. 45: 1323–1336.
• Ito T., K. Okuma, X.X. Ma, H. Yuzawa and K. Hiramatsu. 2003. Insights on antibiotic resistance of Staphylococcus aureus from its whole genome: genomic island SCC. Drug. Resist. Updat. 6: 41–52.
• Ito T., X.X. Ma, F. Takeuchi, K. Okuma, H. Yuzawa and K. Hiramatsu. 2004. Novel type V staphylococcal cassette chromosome mec driven by a novel cassette chromosome recombinase,ccrC. Antimicrob. Agents. Chemother. 48: 2637–2651.
• IWG-SCC. 2009. Classification of staphylococcal cassette chromosome mec (SCCmec): guidelines for reporting novel SCCmec elements. Antimicrob. Agents. Chemother. 53: 4961–4967.
• Katayama Y., T. Ito and K. Hiramatsu. 2000. A new class of genetic element, staphylococcus cassette chromosome mec, encodes methicillin resistance in Staphylococcus aureus. Antimicrob. Agents. Chemother. 44: 1549–1555.
• Katayama Y., T. Ito and K. Hiramatsu. 2001. Genetic organization of the chromosome region surrounding mecA in clinical staphylococcal strains: role of IS431-mediated mecI deletion in expression of resistance in mecA-carrying, low-level methicillin-resistant Staphylococcus haemolyticus. Antimicrob. Agents. Chemother. 45: 1955–1963.
• Kondo Y., T. Ito, X.X. Ma, S. Watanabe, B.N. Kreiswirth, J. Etienne and K. Hiramatsu. 2007. Combination of multiplex PCRs for staphylococcal cassette chromosome mec type assignment: rapid identification system for mec, ccr, and major di$erences in junkyard regions. Antimicrob. Agents. Chemother. 51: 264–274.
• Kurt K., A. Alderborn, M. Nilsson, B. Strommenger, W. Witte and U. Nubel. 2009. Multiplexed genotyping of methicillin-resistant Staphylococcus aureus isolates by use of padlock probes and tag microarrays. J. Clin. Microbiol. 47: 577–585.
• Kwon N.H., K.T. Park, J.S. Moon et al. 2005. Staphylococcal cassette chromosome mec (SCCmec) characterization and molecular analysis for methicillin-resistant Staphylococcus aureus and novel SCCmec subtype IVg isolated from bovine milk in Korea. J. Antimicrob. Chemother. 56: 624–632.
• Leski T., D. Oliveira, K. Trzcinski, I.S. Sanches, M. Aires de Sousa, W. Hryniewicz and H. de Lencastre. 1998. Clonal distribution of methicillin-resistant Staphylococcus aureus in Poland. J. Clin. Microbiol. 36: 3532–3539.
• Li S., R.L. Skov, X. Han et al. 2011. Novel types of staphylococcal cassette chromosome mec elements identified in CC398 methicillin resistant Staphylococcus aureus strains. Antimicrob. Agents. Chemother.
• Ma X.X., T. Ito, C. Tiensasitorn, M. Jamklang, P. Chongtrakool, S. Boyle-Vavra, R.S. Daum and K. Hiramatsu. 2002. Novel type of staphylococcal cassette chromosome mec identified in community-acquired methicillin-resistant Staphylococcus aureus strains. Antimicrob. Agents. Chemother. 46: 1147–1152.
• Ma X.X., T. Ito, P. Chongtrakool and K. Hiramatsu. 2006. Predom inance of clones carrying Panton-Valentine leukocidin genes among methicillin-resistant Staphylococcus aureus strains isolated in Japanese hospitals from 1979 to 1985. J. Clin. Microbiol. 44: 4515–4527.
• Malachowa N. and F.R. DeLeo. 2010. Mobile genetic elements of Staphylococcus aureus. Cell. Mol. Life Sci. 67: 3057–3071.
• McCarthy A.J. and J.A. Lindsay. 2010. Genetic variation in Staphylococcus aureus surface and immune evasion genes is lineage associated: implications for vaccine design and host-pathogen interactions. BMC Microbiol. 10: 173.
• McClure J.A., J.M. Conly, S. Elsayed and K. Zhang. 2010. Multiplex PCR assay to facilitate identification of the recently described staphylococcal cassette chromosome mec type VIII. Molecular and cellular probes 24: 229–232.
• Milheirico C., D.C. Oliveira and H. de Lencastre. 2007a. Update to the multiplex PCR strategy for assignment of mec element types in Staphylococcus aureus. Antimicrob. Agents. Chemother. 51: 3374–3377.
• Milheirico C., D.C. Oliveira and H. de Lencastre. 2007b. Multiplex PCR strategy for subtyping the staphylococcal cassette chromosome mec type IV in methicillin-resistant Staphylococcus aureus: ‘SCCmec IV multiplex’. J. Antimicrob. Chemother. 60: 42–48.
• Mongkolrattanothai K., S. Boyle, T.V. Murphy and R.S. Daum. 2004. Novel non-mecA-containing staphylococcal chromosomal cassette composite island containing pbp4 and tagF genes in a commensal staphylococcal species: a possible reservoir for antibiotic resistance islands in Staphylococcus aureus. Antimicrob. Agents. Chemother. 48: 1823–1836.
• O’Brien F.G., G.W. Coombs, J.C. Pearson, K.J. Christiansen and W.B. Grubb. 2005. Type V staphylococcal cassette chromosome mec in community staphylococci from Australia. Antimicrob. Agents. Chemother. 49: 5129–5132.
• Oliveira D.C., A. Tomasz and H. de Lencastre. 2001. The evolution of pandemic clones of methicillin-resistant Staphylococcus aureus: identification of two ancestral genetic backgrounds and the associated mec elements. Microb. Drug. Resist. 7: 349–361.
• Oliveira D.C. and H. de Lencastre. 2002. Multiplex PCR strategy for rapid identification of structural types and variants of the mec element in methicillin-resistant Staphylococcus aureus. Antimicrob. Agents. Chemother. 46: 2155–2161.
• Oliveira D.C., C. Milheirico and H. de Lencastre. 2006a. Redefining a structural variant of staphylococcal cassette chromosome mec, SCCmec type VI. Antimicrob. Agents. Chemother. 50: 3457–3459.
• Oliveira D.C., C. Milheirico, S. Vinga and H. de Lencastre. 2006b. Assessment of allelic variation in the ccrAB locus in methicillinresistant Staphylococcus aureus clones. J. Antimicrob. Chemother. 58: 23–30.
• Shore A., A.S. Rossney, C.T. Keane, M.C. Enright and D.C. Coleman. 2005. Seven novel variants of the staphylococcal chromosomal cassette mec in methicillin-resistant Staphylococcus aureus isolates from Ireland. Antimicrob. Agents. Chemother. 49: 2070–2083.
• Shukla S.K., S.V. Ramaswamy, J. Conradt, M.E. Stemper, R. Reich, K.D. Reed and E.A. Graviss. 2004. Novel polymorphisms in mec genes and a new mec complex type in methicillin-resistant Staphylococcus aureus isolates obtained in rural Wisconsin. Antimicrob. Agents. Chemother. 48: 3080–3085.
• Stephens A.J., F. Huygens and P.M. Giffard. 2007. Systematic derivation of marker sets for staphylococcal cassette chromosome mec typing. Antimicrob. Agents. Chemother. 51: 2954–2964.
• Takano T., W. Higuchi, T. Otsuka et al. 2008. Novel characteristics of community-acquired methicillin-resistant Staphylococcus aureus strains belonging to multilocus sequence type 59 in Taiwan. Antimicrob. Agents. Chemother. 52: 837–845.
• Tsubakishita S., K. Kuwahara-Arai, T. Sasaki and K. Hiramatsu. 2010. Origin and molecular evolution of the determinant of methicillin resistance in staphylococci. Antimicrob. Agents. Chemother. 54: 4352–4359.
• van der Zee A., M. Heck, M. Sterks, A. Harpal, E. Spalburg, L. Kazobagora and W. Wannet. 2005. Recognition of SCCmec types according to typing pattern determined by multienzyme multiplex PCR-amplified fragment length polymorphism analysis of methicillin-resistant Staphylococcus aureus. J. Clin. Microbiol. 43: 6042–6047.
• Wu S.W., H. de Lencastre and A. Tomasz. 2001. Recruitment of the mecA gene homologue of staphylococcus sciuri into a resistance determinant and expression of the resistant phenotype in Staphylococcus aureus. J Bacteriol 183, 2417–2424.
• Yang J.A., D.W. Park, J.W. Sohn and M.J. Kim. 2006. Novel PCRrestriction fragment length polymorphism analysis for rapid typing of staphylococcal cassette chromosome mec elements. J. Clin. Microbiol. 44, 236–238.
• Zhang K., J.A. McClure, S. Elsayed, T Louie and J.M. Conly. 2005. Novel multiplex PCR assay for characterization and concomitant subtyping of staphylococcal cassette chromosome mec types I to V in methicillin-resistant Staphylococcus aureus. J. Clin. Microbiol. 43: 5026–5033.
• Zhang K., J.A. McClure, S. Elsayed and J.M. Conly. 2009. Novel staphylococcal cassette chromosome mec type, tentatively designated type VIII, harboring class A mec and type 4 ccr gene complexes in a Canadian epidemic strain of methicillin-resistant Staphylococcus aureus. Antimicrob. Agents. Chemother. 53: 531–540.