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2009 | 69 | 3 |
Tytuł artykułu

Lack of neuroprotective effect of astroglia-derived BDNF on hippocampal cell cultures exposed to trimethyltin

Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
It is well known that in response to neuronal injury activated glial cells produce pro-infl ammatory cytokines and neurotrophic factors. These immunoregulatory molecules may play either neurotoxic or neuroprotective roles. In previous studies on mixed neuronal-glial cultures of rat hippocampal dentate gyrus we demonstrated that trimethyltin (TMT) induced neuronal cell death accompanied by an enhanced production of tumor necrosis factor alpha (TNF-α) in microglial cells and a strong increase in TNF-α receptor 1 (TNFR1) expression on astrocytes. Since evidence has been provided that TNFR1 could mediate the production of neurotrophic factors, in the current studies we examined whether the brain-derived neurotrophic factor (BDNF) is up-regulated after TMT exposure and whether it may exert neuroprotective effect on dentate granule cells. Using western blot analysis and immunocytochemical staining we have shown a dose-dependent increase in BDNF production, mainly in astrocytes. Quantitative fl uorescence analysis revealed that addition of anti-TNFR1 antibody to TMTtreated cultures suppresses the astroglial synthesis of BDNF. Nevertheless, immunocytochemical studies of active caspase-3 demonstrated the high level of its expression in cultures exposed to TMT, as well as in cultures pre-treated with BDNF. Our data suggest the involvement of TNFR1 pathway in BDNF production in astrocytes and indicate that this neurotrophic factor does not protect granule neurons against TMT injury.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
69
Numer
3
Opis fizyczny
p.339
Twórcy
autor
  • Department of Molecular and Cellular Neurobiology, Nencki Institute of Experimental Biology Polish Academy of Sciences, Warsaw, Poland
  • Department of Molecular and Cellular Neurobiology, Nencki Institute of Experimental Biology Polish Academy of Sciences, Warsaw, Poland
Bibliografia
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-d673894d-656f-4a27-becc-e3d55f3b45b3
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