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2013 | 57 | 3 |

Tytuł artykułu

Assessment of semen quality in infertile dogs using computer-assisted sperm analysis by the Hamilton-Thorne Semen Analyser

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Semen quality parameters of infertile and fertile dogs were compared. Sperm concentration and semen motility parameters were measured by the Hamilton-Thorne Semen Analyser IVOS 12.3. The spermatozoal morphology and the percentage of live spermatozoa were examined microscopically. Forty-six dogs of various breeds were examined. Twenty dogs had a conception failure within last year. These dogs had a history of prior normal fertility. Twenty six fertile dogs served as control. All animals underwent a clinical examination as well as ultrasonography. Sperm concentration was significantly lower in infertile dogs than in fertile dogs. For most determined motility parameters (MOT, PMOT, VAP, VSL, VCL, BCF, RAPID, STATIC) significant differences between infertile and fertile dogs were found. The percentage of spermatozoa with normal morphology also significantly differed between both groups. Ultrasonography of the prostate gland and testes revealed no pathological conditions. The testicular degeneration was assumed to be a possible cause of infertility in these dogs. The present study showed that the most sperm CASA motility parameters were significantly lower in infertile dogs in comparison to the fertile ones, and confirmed the usefulness of the Hamilton-Thorne Semen Analyser for a quick and objective analysis of sperm concentration and motility in dogs.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

57

Numer

3

Opis fizyczny

p.429-432,ref.

Twórcy

  • Department of Animal Reproduction with Clinic, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, 10-719 Olsztyn, Poland
autor
  • Department of Animal Reproduction with Clinic, Faculty of Veterinary Medicine, University of Warmia and Mazury in olsztyn, 10-719 Olsztyn, Poland
autor
  • Department of Reproduction and Clinic for Farm Animals, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, 50-366 Wroclaw, Poland
autor
  • Department of Animal Reproduction with Clinic, Faculty of Veterinary Medicine, University of Warmia and Mazury in olsztyn, 10-719 Olsztyn, Poland

Bibliografia

  • 1. Donnely E.D., Lewis S.E.M., McNally J.A., Thompson W.: In vivo fertilization and pregnancy rates: the influence of sperm motility and morphology on IVF outcome. Fert Steril 1998, 70, 304-314.
  • 2. Farrel P.B., Presicce G.A., Brocektt C.C., Foote R.H.: Quantification of bull sperm characteristics measured by computer-assisted sperm analysis (CASA) and their relationship to fertility. Theriogenology 1998, 49, 871-879.
  • 3. Feldmann J., Nelson R.W.: Canine and feline endocrinology and reproduction. Edited by W.B. Saunders, Philadelphia 1996, pp. 718-733.
  • 4. Fetterlof P.M., Rogers B.J.: Prediction of human sperm penetrating ability using computerized motion parameters. Mol Reprod Dev 1990, 27, 326-331.
  • 5. Fontbonne A.: Infertility in male dogs: recent advances. Rev Bras Reprod Anim 2011, 35, 266-273.
  • 6. Holt C., Holt W.V., Moore H.D., Reed H.C., Curnock R.M.: Objectively measured boar sperm motility parameters correlate with the outcomes of on-farm inseminations: results of two fertility trials. J Androl 1997, 18, 312-323.
  • 7. Iguer-ouada M., Verstegen J.: Evaluation of the Hamilton Thorn computer based automated system for dog semen analysis. Theriogenology 2001, 55, 733-749.
  • 8. Johnston S.D., Root-Kustritz M.V., Olson P.N.S.: Canine and feline theriogenology. Edited by W.B. Saunders, Philadelphia 2001, p. 592.
  • 9. Klimowicz M., Nizanski W., Savic M., Zbyryt L., Dubiel A.: Evaluation of dog semen quality using a conventional microscopic method, flow cytometry and HTM IVOS computer sperm quality analyzer. Med Weter 2005, 61, 1250-1255.
  • 10. Linde-Forsberg C.: Achieving canine pregnancy using frozen and chilled extended semen. Vet Clin North Am Small Anim Pract 2001, 21, 467-485.
  • 11. Mickelsen W.D., Memon M.A., Anderson B.P., Freeman D.A.: The relationship of semen quality to pregnancy rate and litter size following artificial insemination in the bitch. Theriogenology 1993, 39, 553-560.
  • 12. Milani C., Fontbonne A., Sellem E., Steletta C., Gerard O., Romagnoli S.: Effect of post-thaw dilution with caffeine, pentoxifylline, 2'-deoxyadenosine and prostatic fluid on motility of frozen post-thawed dog semen. Theriogenology 2010, 74, 153-164.
  • 13. Mickelsen W.D., Memon M.A., Anderson P.B., Freeman D.A.: The relationship of semen quality to pregnancy rate and litter size following artificial insemination in the bitch. Theriogenology 1993, 39, 553-560.
  • 14. Mortimer D., Shu M.A., Tan R.: Standarization and quality control of sperm concentration and sperm motility counts in semen analysis. Hum Reprod 1986, 1, 299-303.
  • 15. Niżański W., Klimowicz M., Partka A., Savic M., Dubiel A.: Effects of inclusion of Equex STM into Tris-based extender on the motility of dog spermatozoa incubated at 5 degrees C. Reprod Dom Anim 2009, 44, 363-365.
  • 16. Niżański W., Partyka A., Ochota M., Antończyk A., Mikołajewska N., Błasiak K., Mila H., Stańczyk E.: Flow cytometric, computer assisted and traditional sperm analysis in fertile and subfertile dogs. Proc. 14th EVSSAR Congress, Milano 2011, p. 52.
  • 17. Niżański W., Partyka A., Rijsselaere T.: Use of fluorescent stainings and flow cytometry for canine semen assessment. Reprod Dom Anim 2012, 47 (Suppl. 6), 215-221.
  • 18. Oettle E.E.: Sperm morphology and fertility in the dog. J Reprod Fert Suppl 1993, 47, 257-260.
  • 19. Rijselaere T., Van Soom A., Tanghe S., Coryn M., Maes D., de Kruif A.: New techniques for the assessment of canine semen quality: a review. Theriogenology 2005, 64, 706-719.
  • 20. Rijselaere T., Maes D., Hoflack G., de Kruif A., Van Soom A.: Effect of body weight, age and breeding history on canine sperm quality parameters measured by the Hamilton-Thorne analyser. Reprod Dom Anim 2007, 42, 143-148.
  • 21. Rijselaere T., Van Soom A., Maes D., Niżański W.: Computer assisted sperm analysis in dogs and cats: An uptade after 20 years. Reprod Dom Anim 2012, 47 (Suppl. 6), 204-207.
  • 22. Rodriguez-Martinez H.: Can we increase the estimate value of semen assessment? Reprod Dom Anim 2006, 41 (Suppl. 2), 2 10.
  • 23. Rota A., Milani C., Cabianca G., Martini M.: Comparison between glycerol and ethylene glycol for dog semen cryopreservation. Theriogenology 2006, 65, 1848 1858.
  • 24. Sallam H.N., Ezzeldin F., Sallam A., Agameya A.F., Farrag A.: Sperm velocity and morphology, female characteristics, and the hypo-osmotic swelling test as predictors of fertilization potential: experience from the IVF model. Int J Fertil Womens Med 2003, 48, 88-95.
  • 25. Schäfer S., Aurich Ch.: Use of new computer -assisted sperm analyzer for the assessment of motility and viability of dog spermatozoa and evaluation of four different semen extenders for predilution. Anim Reprod Sci 2007, 102, 1 -13.
  • 26. Verstegen J., Iguer-Ouada M., Onclin M.: Computer assisted semen analyzers in andrology research and veterinary practice. Theriogenology 2002, 57, 149-179.

Typ dokumentu

Bibliografia

Identyfikatory

ISBN
10.2478/bvip-2013-0074

Identyfikator YADDA

bwmeta1.element.agro-d672957f-7200-4571-b743-bad4917fd9e6
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