Evaluation of oxidative status in dogs with anemia

• Autorzy: Gultekin M. , Voyvoda H.
• Języki publikacji: EN

Abstrakty

EN

The aim of the present study was to evaluate the relationship between the oxidative status and the severity and type of anemia in dogs. A total of 70 dogs of various breeds, ages and of both sexes were enrolled in the study. Fifty dogs with anemia were classified according to the severity of anemia as mildly (n = 18), moderately (n = 18) or severely (n = 14) anemic on the basis of the hematocrit (HCT) value. Anemia in the same dogs was also classified according to the type as regenerative (n = 26) or non-regenerative (n = 24) on the basis of the absolute reticulocyte count. Twenty dogs were used as healthy control. Total oxidant status (TOS), total antioxidant capacity (TAC) and malondialdehyde (MDA) values in plasma as well as superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) activities in erythrocyte hemolysate were measured to evaluate the oxidative status. The plasma TOS value was increased in all anemic dogs, irrespective of the severity of anemia, whereas a significant increase in the TAC value and a significant decrease in erythrocyte GSH-Px activity were observed in mild and moderate anemia. Plasma TOS and TAC values were higher in dogs with both types of anemia when compared to the healthy group. A significant increase in plasma MDA concentration and a significant decrease in GSH-Px activity were observed in the non-regenerative anemia group. There were moderate negative correlations between HCT and TOS values in the mild anemia group and between HCT and erythrocyte GSH-Px activity in the regenerative anemia group. In conclusion, oxidative stress develops in dogs with anemia, and it is largely independent of the severity and type of anemia. These results suggest that further studies with different etiologies may also be useful for evaluating the efficacy of antioxidants administered at different doses and in different combinations to treat anemia in dogs.

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2017
• Tom: 73
• Numer: 08
• Opis fizyczny: p.496-499,ref.

Twórcy

autor

Gultekin M.

• Department of Internal Medicine, Faculty of Veterinary Medicine, Adnan Menderes University, Isikli, Aydin, Turkey

autor

Voyvoda H.

• Department of Internal Medicine, Faculty of Veterinary Medicine, Adnan Menderes University, Isikli, Aydin, Turkey

Bibliografia

• Almeida B. F. M., Narciso L. G., Melo L. M., Preve P. P., Bosco A. M., Lima V. M. F., Ciarlini P. C.: Leishmaniasis causes oxidative stress and alteration of oxidative metabolism and viability of neutrophils in dogs. Vet. J. 2013, 198, 599-605.
• Aslan M., Horoz M., Celik H.: Evaluation of oxidative status in iron deficiency anemia through total antioxidant capacity measured using an automated method. Turk. J. Hematol. 2011, 28, 42-46.
• Chapple I. L.: Reactive oxygen species and antioxidants in inflammatory diseases. J. Clin. Periodontol. 1997, 24, 287-296.
• Chaudhuri S., Varshney J. P., Patra R. C.: Erythrocytic antioxidant defense, lipid peroxides level and blood iron, zinc and copper concentrations in dogs naturally infected with Babesia gibsoni. Res. Vet. Sci. 2008, 85, 120-124.
• Cowgill E. S., Neel J. A., Grindem C. B.: Clinical application of reticulocyte counts in dogs and cats. Vet. Clin. North. Am. Small. Anim. Pract. 2003, 33, 1223-1244.
• Erel O.: A new automated colorimetric method for measuring total oxidant status. Clin. Biochem. 2005, 38, 1103-1111.
• Erel O.: A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clin. Biochem. 2004, 37, 277-285.
• Grimes C. N., Fry M. M.: Nonregenerative anemia: mechanisms of decreased or ineffective erythropoiesis. Vet. Pathol. 2015, 52, 298-311.
• Grotto D., Santa Maria L., Valentini J., Paniz C., Schmitt G., Garcia S. C.: Importance of the lipid peroxidation biomarkers and methodological aspects for malondialdehyde quantification. Quim. Nova 2009, 32, 169-174.
• Harvey W. J.: Erythrocyte biochemistry, [in:] Weiss D. J., Wardrop K. J.: Schalm’s Veterinary Hematology. 6th Edition. Blackwell Publishing, Iowa, USA 2010, p. 131-135.
• Hess J. R.: Red cell storage. J. Proteomics. 2010, 73, 368-373.
• Iuchi Y.: Anemia caused by oxidative stress, [in:] Silverberg D.: Anemia. InTech, Croatia 2012, p. 49-62.
• Khanna A., Dubey M., Sinha R.: Oxidative stress and its correlation with severity of maternal anemia. Indian. J. Prev. Soc. Med. 2010, 41, 231-236.
• Kogika M. M., Lustoza M. D., Hagiwara M. K., Caragelasco D. S., Martorelli C. R., Mori C. S.: Evaluation of oxidative stress in the anemia of dogs with chronic kidney disease. Vet. Clin. Path. 2014, 44, 70-78.
• Lanevschi A., Wardrop K. J.: Principles of transfusion medicine in small animals. Can. Vet. J. 2001, 42, 447-454.
• Lang F., Abed M., Lang E., Föller M.: Oxidative stress and suicidal erythrocyte death. Antioxid. Redox. Signal. 2014, 21, 138-153.
• Lang F., Lang K. S., Lang P. A., Huber S. M., Wieder T.: Mechanisms and significance of eryptosis. Antioxid. Redox. Signal. 2006, 8, 1183-1192.
• Longoni S. S., Sánchez-Moreno M., López J. E. R., Marín C.: Leishmania infantum secreted iron superoxide dismutase purification and its application to the diagnosis of canine Leishmaniasis. Comp. Immunol. Microbiol. Infect. Dis. 2013, 36, 499-506.
• Luck H.: Catalase, [in:] Bergmeyer H. U.: Methods of enzymatic assays. Academic Press, New York, USA 1965, p. 885-894.
• Madhikarmi N. L., Murthy K. R. S.: Antioxidant enzymes and oxidative stress in the erythrocytes of iron deficiency anemic patients supplemented with vitamins. Iran Biomed. J. 2014, 18, 82-87.
• Mills J.: Anaemia, [in:] Day M. J., Kohn B.: BSAVA Manual of Canine and Feline Haemotology and Transfusion Medicine, 2nd Edition. BSAVA, England 2012, p. 31-44.
• Mohanty J. G.: Red blood cell (RBC) oxidative stress contributes to reduced RBC deformability and oxygen delivery leading to the occurrence of anemia. J. Blood Disord. Transfus. 2013, 4, 58-66.
• Murase T., Ueda T., Yamato O., Tajima M., Maede Y.: Oxidative damage and enhanced erythrophagocytosis in canine erythrocytes infected with Babesia gibsoni. J. Vet. Med. Sci. 1996, 58, 259-261.
• Nagababu E., Gulyani S., Earley C. J., Cutler R. G., Mattson M. P., Rifkind J. M.: Iron-deficiency anaemia enhances red blood cell oxidative stress. Free Radic. Res. 2008, 42, 824-829.
• Niedzwiedz A., Jaworski Z.: Oxidant-antioxidant status in the blood of horses with symptomatic Recurrent Airway Obstruction (RAO). J. Vet. Intern. Med. 2014, 28, 1845-1852.
• Otsuka Y., Yamasaki M., Yamato O., Maede Y.: The effect of macrophages on the erythrocyte oxidative damage and the pathogenesis of anemia in Babesia gibsoni-infected dogs with low parasitemia. J. Vet. Med. Sci. 2002, 64, 221-226.
• Tanja P., Alenka N. S., Butinar J., Nataša T., Marija P., Bettina K., Kessler M.: Antioxidant status in canine cancer patients. Acta Vet. (Beogr). 2008, 58, 275-286.
• Thrall M. A.: Classification of and diagnostic approach to anemia, [in:] Thrall M. A., Weiser G., Allison R., Campbell T. W.: Veterinary Hematology and Clinical Chemistry, 2nd Edition. Wiley-Blackwell, USA 2012, p. 75-113.
• Tvedten H.: Laboratory and clinical diagnosis of anemia, [in:] Weiss D. J., Wardrop K. J.: Schalm’s Veterinary Hematology, 6th Edition. Blackwell Publishing, Iowa, USA 2010, s. 152-161.
• Yamasaki M., Otsuka Y., Yamato O., Tajima M., Maede Y.: The cause of the predilection of Babesia gibsoni for reticulocytes. J. Vet. Med. Sci. 2000, 62, 737-741.
• Yoshoiko T., Kawada K., Shimada T.: Lipid peroxidation in maternal and cord blood and protective mechanism against active oxygen toxicity in the blood. Am. J. Obstet. Gynecol. 1979, 135, 372-376.

Identyfikatory

DOI

10.21521/mw.5754