The effect of calpastatin polymorphism (CAST/HinfI and CAST/Hpy188I) and its interaction with RYR1 genotypes on carcass and pork quality of crossbred pigs

• Autorzy: Rybarczyk A. , Kmiec M. , Napierala F. , Natalczyk-Szymkowska W.
• Języki publikacji: EN

Abstrakty

EN

The aim of the study was to recognize the polymorphism in the calpastatine genes (CAST/Hindi and CAST/Hpy188I) and in the ryanodine receptor gene (RYR1) as well as to establish a possibile linkage between the genes variants and carcass and pork quality traits in crossbreds of German Landrace × German Large White or Leicoma × German Large White sows with Pietrain boars. In terms of carcass and pork quality, no significant differences were found between the genotypes CT and CC at the locus RYR1, as well as between AA and AB genotypes at the locus CAST/Hpy188I. On the other hand, a significant effect was identified of the CAST/HinfI polymorphism on pork quality traits. The meat of AB pigs showed a significantly higher pH, lower drip loss and thermal drip,lower WHC, and lower redness and yellowness of colour as compared to BB animals. Furthermore,a significant effect of interaction CAST/HinfI × RYR1 was found in relation to WHC of meat. The results presented indicate that the CAST gene polymorphism identified by HinfI enzyme may be considered important in terms of meat quality traits of the analysed crossbreds. A follow-up study is necessary, however, involving a larger population that would represent all possible genetic variants of the CAST.

• Wydawca: -
• Czasopismo: Animal Science Papers and Reports
• Rocznik: 2010
• Tom: 28
• Numer: 3
• Opis fizyczny: p.253-260,ref.

Twórcy

autor

Rybarczyk A.

• Department of Evaluation of Livestock Products, Westpomeranian University of Technology, Doktora Judyma 24, 71-466 Szczecin, Poland

autor

Kmiec M.

• Department of Genetics and Animal Breeding, Westpomeranian University of Technology, Doktora Judyma 6, 71-466 Szczecin, Poland

autor

Napierala F.

• Department of Genetics and Animal Breeding, Westpomeranian University of Technology, Doktora Judyma 6, 71-466 Szczecin, Poland

autor

Natalczyk-Szymkowska W.

• Department of Evaluation of Livestock Products, Westpomeranian University of Technology, Doktora Judyma 24, 71-466 Szczecin, Poland

Bibliografia

• AOAC (Association of Official Analytical Chemists), 2003 – Official Methods of Analysis, 17 th Edition., Revision 2, AOAC International, Gaithersburg, USA.
• CIOBANU D.C., BASTIAANSEN J.W.M., LONERGAN S.M., THOMSEN H., DEKKERS J.C.M.,PLASTOW G.S., ROTHSCHILD M.F., 2004 – New alleles in calpastatin gene are associated with meat quality traits in pigs. Journal of Animal Science 82, 2829-2839.
• CIE – COMMISSION INTERNATIONAL DE I’ECLAIRAGE (COLORIMETRY), 1976 – Official Recommendations of the International Commission on Illumination. Publication CIE, No. 15 (E-1.3.1). Bureau Central de la CIE, Paris, France.
• ERNST C.W., ROBIC A., YERLE M., WANG L., ROTHSCHILD M. F., 1998 – Mapping of calpastatin and three microsatellites to porcine chromosome 2q2.1-q2.4. Animal Genetics 29, 212-215.
• FUJII J., OTSU K., ZORZATO F., DE LEON S., KHANNA V.K., WEILER J.E., OBRIEN P.J.,MACLENNAN D.H., 1991 - Identification of a mutation in porcine ryanodine receptor associated with malignant hyperthermia. Science 253, 448-451.
• GLODEK P., KRATZ R., SCHULZ E., FLACHOWSKY G., 2004 – Effect of sire breeds In commercial pig crosses on growth, carcass composition, meat and fat quality. Archiv für Tierzucht Dummerstorf 47, 59-74.
• GOLL D.E., THOMPSON V.F., TAYLOR R.G., OUALI A., 1998 – The calpain system and skeletal muscle growth. Canadian Journal of Animal Science 78, 503-512.
• GRAU R., HAMM R., 1952 – Eine einfache Methode zur Bestimmung der Wasserbindung in Fleisch.Fleischwirtschaft 4, 295-297.
• HONIKEL K.O., 1987 – pH and water binding of meat. Fleischwirtschaft 67, 1098-1102.
• KAPELAŃSKI W., GRAJEWSKA S., KURYŁ J., BOCIAN M., JANKOWIAK H., WIŚNIEWSKA J., 2004 – Calpastatin (CAST) gene polymorphism and selected meat quality traits in pigs. Animal Science Papers and Reports 22, 435-441.
• KOĆWIN-PODSIADŁA M., KURYŁ J., 2003 – The effect of interaction between genotypes at loci CAST, RYR1 and RN on pig carcass quality and pork traits – a review. Animal Science Papers and Reports 21, Supplement, 61-75.
• KOĆWIN-PODSIADŁA M., KURYŁ J., KRZĘCIO E., ANTOSIK K., ZYBERT A., SIECZKOWSKA H., 2004 – An association between genotype at the CAST (calpastatin) locus and carcass quality traits in porkers free of RYR1T allele. Animal Science Papers and Reports 22, 497-505.
• KOĆWIN-PODSIADŁA M., KURYŁ J. KRZĘCIO E., ZYBERT A., PRZYBYLSKI W., 2003 –The interaction between calpastatin and RYR1 genes for some pork quality traits. Meat Science 65,731-735.
• KOOHMARAIE M., WHIPPLE G., KRETCHMAR D.H., CROUSE J.D., MERSMANN H.J., 1991 – Postmortem proteolysis in longissimus muscle from beef, lamb, and pork carcasses. Journal of Animal Science 69, 617-624.
• KOTIK T., 1974 – Zawartość białka w ekstraktach wodnych mięsa jako wskaźnik jego jakości (Protein content in water extracts of meat as an index of its quality) In Polish, summary in English.Roczniki Instytutu Przemysłu Mięsnego 12, 47-52.
• KRZĘCIO E., KOĆWIN-PODSIADŁA M., KURYŁ J., ANTOSIK K., ZYBERT A., SIECZKOWSKA H., POSPIECH E., ŁYCZYŃSKI A., GRZEŚ B., 2004 – An association between genotype at the CAST locus (calpastatin) and meat quality traits in porkers free of RYR1T allele. Animal Science Papers and Reports 22, 489-496.
• KURYŁ J., KAPELAŃSKI W., PIERZCHAŁA M., GRAJEWSKA S., BOCIAN M., 2003 –Preliminary observations on the effect of calpastatin gene (CAST) polymorphism on carcass traits In pigs. Animal Science Papers and Reports 21, 87-95.
• KURYŁ J., KRZĘCIO E. KOĆWIN-PODSIADŁA M., MONIN G., 2004 – The influence of CAST and RYR1 genes polymorphism and their interactions on selected meat quality parameters in fourbreed fatteners. Animal Science Papers and Reports 22, 479-488.
• KUSEC G., BAULAIN U., HENNING M., KÖHLER P., KALLWEIT E., 2005 – Fattening, carcass,and meat quality traits of hybrid pigs as influenced by MHS genotype and feeding systems. Archiv für Tierzucht Dummerstorf 48, 40-49.
• MELODY J.L., LONERGAN S.M., ROWE L.J., HUIATT T.W., MAYES M.S. HUFF-LONERGAN E., 2004 – Early post mortem biochemical factors influence tenderness and water-holding capacity of three porcine muscle. Journal of Animal Science 82, 1195-1205.
• POHJA M.S., NIINIVAARA F.P., 1957 – Die Bestimmung der Wasserbindung des Fleisches mittels der Konstantdrückmethode. Fleischwirtschaft 9, 193-195.