Identification of Ephedra species by phylogenetic analyses using matK and ITS1 sequences

• Autorzy: Zhao Y.S. , Xie L.X. , Mao F.Y. , Cao Z. , Wang W.P. , Zhao Q.P. , Zhang X.H.
• Języki publikacji: EN

Abstrakty

EN

In this study, the species identifications of seven Ephedra plants, including three medicinal plants from the Pharmacopoeia of the People’s Republic of China, were conducted using phylogenetic analyses, and the method’s validity was verified. The phylogenetic trees constructed from the maturase-coding gene (matK) and internal transcribed spacer 1 (ITS1) sequences showed that the former could be used for identifying five Ephedra plants, Ephedra intermedia, E. equisetina, E. antisyphilitica, E. major, and E. aphylla, but it had less power to discriminate E. sinica and E. przewalskii, while the latter could distinguish five Ephedra plants, E. przewalskii, E. equisetina, E. antisyphilitica, E. major, and E. aphylla, but it had less power to discriminate E. sinica and E. intermedia. However, when the two genes were combined, the seven species could be completely distinguished from each other, especially the medicinal plants from the others, which is significant in developing their pharmaceutical uses and in performing quality control assessments of herbal medicines. The method presented here could be applied to the analysis of processed Ephedra plants and to the identification of the botanical origins of crude drugs. Additionally, we discovered that E. equisetina and E. major were probably closely related to each other, and that E. sinica, E. intermedia, and E. przewalskii also had a close genetic relationship.

• Wydawca: -
• Czasopismo: Acta Societatis Botanicorum Poloniae
• Rocznik: 2016
• Tom: 85
• Numer: 2
• Opis fizyczny: Article 3497 [9p.], fig.,ref.

Twórcy

autor

Zhao Y.S.

• Ningxia Medical University Pharmacy College, Yinchuan 750004, Ningxia, China
• Ningxia Research Center of Modern Hui Medicine Engineering and Technology, Yinchuan 750004, Ningxia, China
• Ministry of Education Key Laboratory of Modern Hui Chinese Medicine, Yinchuan 750004, Ningxia, China

autor

Xie L.X.

• Ningxia Medical University Pharmacy College, Yinchuan 750004, Ningxia, China
• Ningxia Research Center of Modern Hui Medicine Engineering and Technology, Yinchuan 750004, Ningxia, China

autor

Mao F.Y.

• Ningxia Medical University Pharmacy College, Yinchuan 750004, Ningxia, China

autor

Cao Z.

• Ningxia Medical University Pharmacy College, Yinchuan 750004, Ningxia, China
• Ningxia Research Center of Modern Hui Medicine Engineering and Technology, Yinchuan 750004, Ningxia, China

autor

Wang W.P.

• Ningxia Medical University Pharmacy College, Yinchuan 750004, Ningxia, China
• Ningxia Research Center of Modern Hui Medicine Engineering and Technology, Yinchuan 750004, Ningxia, China

autor

Zhao Q.P.

• Ningxia Medical University Pharmacy College, Yinchuan 750004, Ningxia, China
• Ningxia Research Center of Modern Hui Medicine Engineering and Technology, Yinchuan 750004, Ningxia, China
• Ministry of Education Key Laboratory of Modern Hui Chinese Medicine, Yinchuan 750004, Ningxia, China

autor

Zhang X.H.

• Ningxia Medical University Pharmacy College, Yinchuan 750004, Ningxia, China
• Ningxia Research Center of Modern Hui Medicine Engineering and Technology, Yinchuan 750004, Ningxia, China

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Identyfikatory

DOI

10.5586/asbp.3497