Cloning, characterization and overexpression of the complete open reading frame of the lateral shoot-inducing factor from Jatropha Curcas

• Autorzy: Zong H. , Wang S. , Ouyang C. , Wang W. , Li L. , Chen F.
• Języki publikacji: EN

Abstrakty

EN

Shoot branching is considered a key factor that influences plant architecture and productivity. In the present study, the open reading frame encoding lateral shootinducing factor, named JLIF, was cloned from Jatropha curcas L. JLIF encoded a 214 amino acid polypeptide and contained a strongly conserved QALGGH motif in zincfinger domain which is the typical motif of the SUP protein family. Sequence and phylogenetic analysis indicated that JLIF had very high similarity (97.67%) to the LIF gene in petunia, but showed no significant similarity to other known SUP proteins except for the conserved motifs. Taken together, we presumed JLIF was the ortholog of LIF protein, and both appeared to belong to a novel subfamily of the SUP proteins. Semi- quantitative RT-PCR analysis revealed JLIF was abundant in stems and petioles, weakly expressed in leaves and seeds, but absent in roots. Subsequently, the JLIF gene was introduced into tobacco under the control of the Cauliflower Mosaic Virus (CaMV) 35S promoter. PCR analysis and Southern blot hybridization were performed to confirm if JLIF was stably integrated into the tobacco genome. GUS activity analysis and RTPCR revealed the location of the overexpressed JLIF. Overexpression of JLIF in transgenic tobaccos resulted in reduced plants height and shortened internodes in vitro.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2012
• Tom: 34
• Numer: 1
• Opis fizyczny: p.317-326,fig.,ref.

Twórcy

autor

Zong H.

• Faculty of Architecture, Southwest Jiaotong University, 610031 Chengdu, China
• College of Life Sciences, Sichuan University, 610064 Chengdu, China

autor

Wang S.

• College of Life Sciences, Sichuan University, 610064 Chengdu, China

autor

Ouyang C.

• College of Life Sciences, Sichuan University, 610064 Chengdu, China

autor

Wang W.

• College of Life Sciences, Sichuan University, 610064 Chengdu, China

autor

Li L.

• College of Life Sciences, Sichuan University, 610064 Chengdu, China

autor

Chen F.

• College of Life Sciences, Sichuan University, 610064 Chengdu, China

Bibliografia

• Abdelgadir HA, Johnso SD, Staden JV (2009) Promoting branching of a potential biofuel crop Jatropha curcas L. by foliar application of plant growth regulators. Plant Growth Regul 58:287–295. doi:10.1007/s10725-009-9377-9
• Adebowale KO, Adedire CO (2006) Chemical composition and insecticidal properties of the underutilized Jatropha curcas seed oil. Afr J. Biotechnol 5:901–906
• Aguilar-Martinez JA, Poza-Carrion C, Cubas P (2007) Arabidopsis BRANCHED1 acts as an integrator of branching signals within axillary buds. Plant Cell 19:458–472. doi:10.1105/tpc.106. 048934
• Arite T, Iwata H, Ohshima K, Maekawa M, Nakajima M, Kojima M, Sakakibara H, Kyozuka J (2007) DWARF10, an RMS1/MAX4/ DAD1 ortholog, controls lateral bud outgrowth in rice. Plant J 51:1019–1029. doi:10.1111/j.1365-313X.2007.03210.x
• Babb S, Muehlbauer GJ (2003) Genetic and morphological characterization of the barley uniculm2 (cul2) mutant. Theor Appl Genet 106:846–857. doi:10.1007/s00122-002-1104-0
• Bennett T, Leyser O (2006) Something on the side: axillary meristems and plant development. Plant Mol Biol 60:843–854. doi:10. 1007/s11103-005-2763-4
• Booker J, Auldridge M, Wills S, McCarty D, Klee H, Leyser O (2004) MAX3/CCD7 is a carotenoid cleavage dioxygenase required for the synthesis of a novel plant signaling molecule. Curr Biol 14:1232–1238. doi:10.1016/j.cub.2004.06.061
• Cervera M, Pina JA, Juárez J, Navarro L, Peña L (2000) A broad exploration of a transgenic population of citrus: stability of gene expression and phenotype. TAG Theor Appl Genet 100:670–677. doi:10.1007/s001220051338
• Chrispeels HE, Oetttinger H, Janvier N, Tague BW (2000) AtZFP1, encoding Arabidopsis thaliana C2H2 zinc-finger protein 1, is expressed downstream of photomorphogenic activation. Plant Mol Biol 42:279–290. doi:10.1023/A:1006352809700
• Cline MG (1991) Apical dominance. Bot. Rev 57:318–358. doi: 10.1007/BF02858771
• Dinkins R, Pflipsen C, Thompson A, Collins GB (2002) Ectopic expression of an Arabidopsis single zinc finger gene in tobacco results in dwarf plants. Plant Cell Physiol 43:743–750. doi: 10.1093/pcp/pcf086
• Dominguez A, Guerri J, Cambra M, Navarro L, Moreno P, Peña L (2000) Efficient production of transgenic citrus plants expressing the coat protein gene of citrus tristeza virus. Plant Cell Rep 19:427–433. doi:10.1007/s002990050751
• Dutta I, Majumder P, Saha P, Ray K, Das S (2005) Constitutive and phloem specific expression of Allium sativum leaf agglutinin (ASAL) to engineer aphid (Lipaphis erysimi) resistance in transgenic Indian mustard (Brassica juncea). Plant Sci 169:996–1007. doi:10.1016/j.plantsci.2005.05.016
• Gour VK (2006) Production practices including post-harvest management of J. curcas. In: Singh B, Swaminathan R, Ponraj V (eds) Biodiesel conference toward energy independence-focus of Jatropha. Rashtrapati Bhawan, New Delhi, India, pp 223–251
• Hicks CR, Smith HMS, Shieh M, Raikhel NV (1995) Three classes of nuclear import signals bind to plant nuclei. Plant Physiol 107:1055–1058
• Hiratsu K, Ohta M, Matsui K, Ohme-Takagi M (2002) The SUPERMAN protein is an active repressor whose carmotifyterminal repression domain is required for the development of normal flowers. FEBS Lett 514:351–354. doi:10.1016/S0014-5793(02)02435-3
• Hoekema A, Hirsch PR, Hooykaas PJJ, Schilperoort RA (1983) A binary plant vector strategy based on separation of vir- and T-region of the Agrobacterium tumefaciens Ti-plasmid. Nature 303:179–180. doi:10.1038/303179a0
• Holsters M, de Waele D, Depicker A, Messens E, van Montagu M, Schell J (1978) Transfection and transformation of Agrobacterium tumefaciens. Mol Gen Genet 163:181–187. doi:10.1007/ BF00267408
• Horsch RB, Fry JE, Hoffmann NL, Eichholtz D, Rogers SG, Fraley RT (1985) A simple and general method for transferring genes into plants. Science 227:1229–1231. doi:10.1126/science.227. 4691.1229
• Hubbard L, McSteen P, Doebley J, Hake S (2002) Expression patterns and mutant phenotype of teosinte branched1 correlate with growth suppression in maize and teosinte. Genetics 162:1927–1935
• Ishikawa S, Maekawa M, Arite T, Onishi K, Takamure I, Kyozuka J (2005) Suppression of tiller bud activity in tillering dwarf mutants of rice. Plant Cell Physiol 46:79–86. doi:10.1093/pcp/ pci022
• Kandpal JB, Mira M (1995) Jatropha curcus: a renewable source of energy for meeting future energy needs. Renew Energy 6:159–160
• Kumar S, Fladung M (2000) Transgene repeats in aspen: molecular characterisation suggests simultaneous integration of independent T-DNAs into receptive hotspots in the host genome. Mol Gener Genet MGG 264:20–28. doi:10.1007/s004380000296
• Kumar S, Fladung M (2001) Gene stability in transgenic aspen (Populus).II. Molecular characterization of variable expression of transgene in wild and hybrid aspen. Planta 213:731–740. doi: 10.1007/s004250100535
• Kumar S, Fladung M (2002) Transgene integration in aspen: structures of integration sites and mechanism of T-DNA integration. Plant J 31:543–551. doi:10.1046/j.1365-313X.2002. 01368.x
• Lewis JM, Mackintosh CA, Shin S, Gilding E, Kravchenko S, Baldridge G, Zeyen R, Muehlbauer GJ (2008) Overexpression of the maize Teosinte Branched1 gene in wheat suppresses tiller development. Plant Cell Rep 27:1217–1225. doi:10.1007/s00299-008-0543-8
• Li X, Qian Q, Fu Z, Wang Y, Xiong G, Zeng D, Wang X, Liu X, Teng S, Hiroshi F (2003) Control of tillering in rice. Nature 422:618–621. doi:10.1038/nature01518
• Long J, Barton MK (2000) Initiation of axillary and floral meristems in Arabidopsis. Dev Biol 218:341–353. doi:10.1006/dbio. 1999.9572
• Mazumdar P, Basu A, Paul A, Mahanta C, Sahoo L (2010) Age and orientation of the cotyledonary leaf explants determine the efficiency of de novo plant regeneration and Agrobacterium tumefacien-s mediated transformation in Jatropha curcas L. S Afr J Bot 76:337–344. doi:10.1016/j.sajb.2010.01.001
• McSteen P, Leyser O (2005) Shoot branching. Annu Rev Plant Biol 56:353–374. doi:10.1146/annurev.arplant.56.032604.144122
• Murray MG, Thompson WF (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res 8:4321–4325. doi:10. 1093/nar/8.19.4321
• Nakagawa H, Jiang CJ, Sakakibara H, Kojima M, Honda I, Hidetoshi A, Nishijima T, Masaji K, Koshioka M, Lewis NM, Takatsuji H (2005) Overexpression of a petunia zinc-finger gene alters cytokinin metabolism and plant forms. Plant J 41:512–523. doi: 10.1111/j.1365-313X.2004.02316.x
• Nordstrom A, Tarkowski P, Tarkowska D, Norbaek R, Astot C, Dolezal K, Sandberg G (2004) Auxin regulation of cytokinin biosynthesis in Arabidopsis thaliana: a factor of potential importance for auxin-cytokinin-regulated development. PNAS 101:8039–8044. doi:10.1073/pnas.0402504101
• Ohta M, Matsui K, Hiratsu K, Shinshi H, Takagi MO (2001) Repression domains of class II ERF transcriptional repressors share an essential motif for active repression. Plant Cell 13:1959–1968. doi:10.1105/tpc.13.8.1959
• Raffeiner B, Winkelmann T, Serek M (2009) Agrobacterium tumefaciens-mediated transformation of Oncidium and Odontoglossum orchid species with the ethylene receptor mutant gene etr1–1. Plant Cell Tiss Organ Cult 98:125–134. doi:10.1007/s11240-009-9545-7
• Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual, 3rd edn. Cold Spring Harbor Laboratory Press, New York
• Sanikhani M, Mibus H, Stummann BM, Serek M (2008) Kalanchoe blossfeldiana plants expressing the Arabidopsis etr1–1 allele show reduced ethylene sensitivity. Plant Cell Rep 27:729–737. doi:10.1007/s00299-007-0493-6
• Satoh-Nagasawa N, Nagasawa N, Malcomber S, Sakai H, Jackson D (2006) A trehalose metabotic enzyme controls inflorescence architecture in maize. Nature 441:227–230. doi:10.1038/ nature04725
• Schumacher K, Schmitt T, Rossberg M, Schmitz G, Theres K (1999) The Lateral suppressor (Ls) gene of tomato encodes a new member of the VHIID protein family. PNAS 96:290–295
• Svitashev SK, Pawlowski WP, Makarevitch I, Plank DW, Somers DA (2002) Complex transgene locus structures implicate multiple mechanisms for plant transgene rearrangement. Plant J 32: 433–445. doi:10.1046/j.1365-313X.2002.01433.x
• Takatsuji H, Matsumoto T (1996) Target-sequence recognition by separate Cys2/His2-type zinc-finger proteins in plants. J. Biol. Chem 271:23368–23373. doi:10.1074/jbc.271.38.23368
• Takeda T, Suwa M, Kitano H, Ueguchi-Tanaka M, Ashikari M, Matsuoka M, Ueguchi C (2003) The OsTB1 gene negatively regulates lateral branching in rice. Plant J 33:513–520. doi: 10.1046/j.1365-313X.2003.01648.x
• Tiwari AK, Kumar A, Raheman H (2007) Biodiesel production from Jatropha (Jatropha curcas) with high free fatty acids: an optimized process. Biomass Bioenerg 31:569–575. doi:10.1016/ j.biombioe.2007.03.003
• Vogel JP, Garvin DF, Leong OM, Hayden DM (2006) Agrobacterium-mediated transformation and inbred line development in the model grass Brachypodium distachyon. Plant Cell Tiss Organ Cult 84:100179–100191. doi:10.1007/s11240-005-9023-9
• Vollbercht E, Springer PS, Goh L, Buckler ES, Martienssen R (2005) Architecture of floral branch systems in maize and related grasses. Nature 436:119–1126. doi:10.1038/nature03892
• Ward SP, Leyser O (2004) Shoot branching. Plant Biol 7:73–78. doi: 10.1016/j.pbi.2003.10.002
• Xu D, Huang J, Guo S, Yang X, Bao Y, Tang H, Zhang H (2008) Overexpression of a TFIIIA-type zinc finger protein gene ZFP252 enhances drought and salt tolerance in rice (Oryza sativa L.). FEBS Lett 582:1037–1043. doi:10.1016/j.febslet. 2008.02.052
• Yoshioka K, Fukushima S, Yamazaki T, Yoshida M, Takatsuji H (2001) The plant zinc-finger protein ZPT2–2 has a unique mode of DNA interaction. J Biol Chem 276:35802–35807. doi: 10.1074/jbc.M104079200
• Zou JH, Zhang SY, Zhang WP, Li G, Chen ZX, Zhai WX, Zhao XF, Pan XB, Xie Q, Zhu LH (2006) The rice HIGH-TILLERING DWARF1 encoding an ortholog of Arabidopsis MAX3 is required for negative regulation of the out growth of axillary buds. Plant J 48:687–696. doi:10.1111/j.1365-313X.2006.02916.x

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