Use of cultured cells of mammal and insect origin to assess cytotoxic effects of the pesticide Chlorpyrifos

• Autorzy: Pollakova J. , Pistl J. , Kovalkovicova N. , Csank T. , Kocisova A. , Legath J.
• Języki publikacji: EN

Abstrakty

EN

In the present study four different cell cultures, derived from rabbit kidney (RK13), rat, and murine liver (WBF344 and Hepa 1c1c7) and insect origin (Sf21) were used to examine the effects of chlorpyrifos. Sf21 cells were the most sensitive to chlorpyrifos, with significant suppression of their proliferative activity ranging from 10⁻¹-10⁻⁵ M. However, significant suppression of proliferative activity also was recorded in mammalian cell cultures Hepa 1c1c7 (10⁻¹-10⁻³ M), WBF344 (10⁻¹-10⁻² M), and RK13 ( 10⁻¹ M). A cytopathic effect and LDH leakage into the medium was observed in RK 13 ( 10⁻¹-10⁻³ M) > WBF344 and Hepa 1c1c7 cells ( 10⁻¹-10⁻² M) > Sf21 ( 10⁻¹ M) compared to solvent control. Our results indicate that chlorpyrifos exposure caused a species-dependent decrease in cell proliferation and cell membrane damage.

Słowa kluczowe

EN

cultured cell; mammalian cell; mammal; insect; cytotoxic effect; pesticide; chlorpyrifos; insecticide; cell culture; cell proliferation

• Wydawca: -
• Czasopismo: Polish Journal of Environmental Studies
• Rocznik: 2012
• Tom: 21
• Numer: 4
• Opis fizyczny: s.1001-1006,ref.

Twórcy

autor

Pollakova J.

• Department of Microbiology and Immunology, University of Veterinary Medicine and Pharmacy, Komenskeho 73, 041 81 Kosice, Slovak Republic

autor

Pistl J.

autor

Kovalkovicova N.

autor

Csank T.

autor

Kocisova A.

autor

Legath J.

Bibliografia

• 1. SAULSBURY M. D., HEYLIGER S. O., WANG K., JOHNSON D. J. Chlorpyrifos induces oxidative stress in oligodendrocyte progenitor cells. Toxicology, 259, (1-2), 1, 2009.
• 2. THE PESTICIDE MANUAL, A World Compendium, Tomlin, C.D.S., 14th ed., British Crop Protection Council: Alton, Hampshire, UK, pp. 186-187, 2006.
• 3. KIDD H., JAMES D. R., Eds. The Agrochemicals Handbook, 3rd. ed., Royal Society of Chemistry Information Services: Cambridge, UK, 5-14, 1991.
• 4. YIN X., ZHU G., LI X. B., LIU S. Genotoxicity evaluation of chlorpyrifos to amphibian Chinese toad (Amphibian: Anura) by Comet assay and Micronucleus test. Mutat. Res., 680,(1-2), 2, 2009.
• 5. BLODGETT D. J. Organophosphate and carbamate insecticides. In: Peterson M. E., Talcott P. A. Small animal toxicology. 2nd. ed., St. Louis: Saunders, pp. 941-947, 2006.
• 6. VISWANATH G., CHATTERJEE S., DABRAL S., NANGUNERI S. R., DIVYA G., ROY P. Anti-androgenic endocrine disrupting activities of chlorpyrifos and piperophos. J. Steroid Biochem. Mol. Biol., 120, (1), 22, 2010.
• 7. ESKENAZI B., MARKS A. R., BRADMAN A., HARLEY K, BARR D. B., JOHNSON C., MORGA N., JEWELL N. P. Organophosphate pesticide exposure and neurodevelopment in young Mexican-American children. Environ. Health Perspect., 115, (5), 792, 2007.
• 8. SAVOLAINEN K. Handbook of Pesticide Toxicology (Second Edition) Chapter 50 - Understanding the Toxic Actions of Organophosphates, pp. 1013-1041, 2001.
• 9. KHAN S. M. Protective effect of black tea extract on the levels of lipid peroxidation and antioxidant enzymes in liver of mice with pesticide-induced liver injury. Cell Biochem. Fund., 24, (4), 327, 2006.
• 10. MANSOUR S. A., MOSSA A. T. Adverse effects of exposure to low doses of chlorpyrifos in lactating rats. Toxicol. Ind. Health., 27, (3), 213, 2011.
• 11. TRIPATHI S., SRIVASTAV A. K. Nephrotoxicity induced by long-term oral administration of different doses of chlorpyrifos. Toxicol. Ind. Health., 26, (7), 439, 2010.
• 12. DAVILA J. C, RODRIGUEZ R. J., MELCHERT R. B., ACOSTA D. Predictive value of in vitro model systems in toxicology. Ann. Rev. Pharmacol. Toxicol., 38, 63,1998.
• 13. LI W., CHOY D. F., LAM M. S., MORGAN T., SULLIVAN M. E., POST J. M. Use of cultured cells of kidney origin to assess specific cytotoxic effects of nephrotoxins. Toxicol, in Vitro, 17, (1), 107, 2003.
• 14. MERSCH-SUNDERMANN V., KNASMULLER S., WU X. J., DARROUDI F., KASSIE F. Use of a human-derived liver cell line for the detection of cytoprotective, antigenotoxic and cogenotoxic agents. Toxicol., 198, 329, 2004.
• 15. TANG Q.-H., YANG W., TAN Y.-X., ZHOU W.-P. Sodium butyrate induces rat liver oval cells WB-F344 differentiating into billiary epithelium cells in vitro. Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings, Article number 4030430, pp. 576- 579, 2006.
• 16. RUMORA L., KOVAČIĆ S., ROZGAJ R., ČEPELAK I., PEPELJNJAK S., ŽANIĆ GRUBIŠIĆ T. Cytotoxic and genotoxic effects of fumonisin B1 on rabbit kidney RK13 cell line. Arch. Toxicol., 76, (1), 55, 2002.
• 17. THORNLEY J. A., TRASK H. W., RIDLEY C. J., KORC M., GUI J., RINGELBERG C. S., WANG S., TOMLINSON C. R. Differential regulation of polysome mRNA levels in mouse Hepa-1C1C7 cells exposed to dioxin. Toxicol, in Vitro, 25, (7), 1457, 2011.
• 18. PARK J. C, PARK B. J., LEE D. H., SUH H., KIM D. G., KWON O. H. Evaluation of the cytotoxicity of polyetherurethane (PU) film containing zinc diethyldithiocarbamate (ZDEC) on various cell lines. Yonsei Med. J., 43, (4), 518, 2002.
• 19. LEE S.-J., NAM K.-W., MAR W. Induction of quinone reductase activity by psoralidin isolated from Psoralen corylifolia in mouse hepa 1c1c7 cells. Arch. Pharmacal. Res., 32, (7), 1061,2009.
• 20. NATSCH A., EMTER R. Skin sensitizers induce antioxidant response element dependent genes: Application to the in vitro testing of the sensitization potential of chemicals. Toxicol. Sci., 102, (1), 110, 2008.
• 21. HSU CH.-H., QUISTAD G. B., CASIDA J. E. Phosphineinduced oxidative stress in Hepa 1c1c7 cells. Toxicol. Sci., 46, 204,1998.
• 22. HAN E. H., JEONG T. C., JEONG H. G. Methoxychlor suppresses the 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)- inducible CYP1A1 expression in murine Hepa-1c1c7 cells. J. Toxicol. Env. Heal. A, 70, (15-16), 1304, 2007.
• 23. CHEN P. - J., MOORE T., NESNOW S. Cytotoxic effects of propiconazole and its metabolites in mouse and human hepatoma cells and primary mouse hepatocytes. Toxicol, in Vitro, 22, (6), 1476, 2008.
• 24. STALJANSSENS D., VAN CAMP J., HERREGODS G., DHAENENS M, DEFORCE D., VAN DE VOORDE J., SMAGGHE G. Antihypertensive effect of insect cells: in vitro and in vivo evaluation. Peptides, 32, (3), 526, 2011.
• 25. POWELL G. F., WARD D. A., PRESCOTT M. C., SPILLER D. G., WHITE M. R„ TURNER P. C., EARLEY F. G., PHILLIPS J., REES H. H. The molecular action of the novel insecticide, Pyridalyl. Insect. Biochem. Mol. Biol., 41, (7), 459, 2011.
• 26. NANDI S., GUPTA P. S., ROY S. C„ SELVARAJU S., RAVINDRA J. P. Chlorpyrifos and endosulfan affect buffalo oocytes maturation, fertilization, and embryo development in vitro directly and through cumulus cells. Environ. Toxicol., 26, (1), 57, 2011.
• 27. PALMA P., PALMA V. L., FERNANDES R. M., BOHN A., SOARES A. M„ BARBOSA I. R. Embryo-toxic effects of environmental concentrations of chlorpyrifos on the crustacean Daphnia magna. Ecotoxicol. Environ. Safi, 72, (6), 1714, 2009.
• 28. SCHEIL V., ZÜRN A„ KÖHLER H. R., TRIEBSKORN R. Embryo development, stress protein (Hsp70) responses, and histopathology in zebrafish (Danio rerio) following exposure to nickel chloride, chlorpyrifos, and binary mixtures of them. Environ. Toxicol., 25, (1), 83, 2010.
• 29. LASSITER T. L., MACKILLOP E. A., RYDE I. T., SEIDLER F. J., SLOTKIN T. A. Is fipronil safer than chlorpyrifos? Comparative developmental neurotoxicity modelled in PC12 cells. Brain Res. Bull., 78, (6), 313, 2009.
• 30. DE ANGELIS S., TASSINARI R., MARANGHI F., EUSEPI A., DI VIRGILIO A., CHIAROTTI F., RICCERI L., VENEROSI P.A., GILARDI E., MORACCI G., CALAMANDREI G., OLIVIERI A., MANTOVANI A. Developmental exposure to chlorpyrifos induces alterations in thyroid and thyroid hormone levels without other toxicity signs in CD-1 mice. Toxicol. Sci., 108, (2), 311, 2009.
• 31. JAMIL K., SHAIK A. P., MAHBOOB M., KRISHNA D. Effect of organophosphorus and organochlorine pesticides (monochrotophos, chlorpyriphos, dimethoate, and endosulfan) on human lymphocytes in vitro. Drug Chem. Toxicol., 27, (2), 133,2004.
• 32. OOSTINGH G. J., WICHMANN G., SCHMITTNER M., LEHMANN I., DUSCHL A. The cytotoxic effects of the organophosphates chlorpyrifos and diazinon differ from their immunomodulating effects. J. Immunotoxicol., 6, (2), 136, 2009.
• 33. LI Q., KOBAYASHI M., KAWADA T. Chlorpyrifos induces apoptosis in human T cells. Toxicology, 255, (1-2), 53, 2009.
• 34. HOOGDUIJN M. J., RAKONCZAY Z., GENEVER P. G. The effects of anticholinergic insecticides on human mesenchymal stem cells. Toxicol. Sci., 94, (2), 342, 2006.
• 35. EL-SHENAWY N. S., AL-EISA R. Mechanism of Organophosphorous Insecticide Chlorpyrifos Toxicity In Isolated Rat Hepatocytes. J. Egypt. Soc. Toxicol., 43,97,2010.
• 36. DAHAMNA S., SEKFALI N., WALKER CH. Biochemical indicators of hepatotoxic effects of pesticides. Commun. Agrie. Appl. Biol. Sci., 69, (4), 821, 2004.
• 37. AKHTAR N., SRIVASTAVA M. K., RAIZADA R. B. Assessment of chlorpyrifos toxicity on certain organs in rat, Rattus norvegicus. J. Environ. Biol., 30, (6), 1047, 2009.
• 38. WANG J., YI CH. H., LIU X. Z., YUAN Y., BIAN J. CH., LIU Z. R Effects of cadmium acetate on oxidative stress in primary cultured rat hepatocytes. Chinese Vet. Sci., 39, 1010, 2009.
• 39. ALY H. A. A., DOMINICA O. Cytotoxicity and mitochondrial dysfunction of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in isolated rat hepatocytes. Tox. Let., 191, 79, 2009
• 40. EL-SHENAWY N. S. Effects of insecticides fenitrothion, endosulfan and abamectin on antioxidant parameters of isolated rat hepatocytes. Toxicol, in Vitro, 24, 1148, 2010.
• 41. CUELLO S., GOYA L., MADRID Y., CAMPUZANO S., PEDRERO M., BRAVO L., CAMARA C., RAMOS S. Molecular mechanisms of methylmercury-induced cell death in human HepG2 cells. Food Chem. Tox., 48, 1405, 2010.
• 42. ŠUTIAKOVÁ I., KOVALKOVlČOVÁ N., LEGÁTH J., PISTL J., KOVÁČ G., ŠUTIAK V., NOVOTNY J., VÁCZI P. Effect of subchronic exposure to tolylfluanid on the lactate dehydrogenase activity and its isoenzymes in sheep. Slovak J. Anim. Sci., 42, 104, 2009.
• 43. WU J., DAUGULIS A. J., FAULKNER P., GOOSEN M. F. A. Correlation of LDH activity with loss of insect cell viability: An assessment of the LDH assay. Biotechnol. Tech., 6, 335, 1992.